Development of Synergy-Based Combination of Methanolic Extract of Andrographis paniculata and Berberis aristata Against E. coli and S. aureus

Abass, Sageer; Zahiruddin, Sultan; Ali, Asghar; Irfan, Mohammad; Jan, Bisma; Haq, Qazi Mohd. Rizwanul; Husain, Syed Akhtar; Ahmad, Sayeed

doi:10.1007/s00284-022-02911-8

Cited by 14 publications

(5 citation statements)

References 44 publications

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“…The inoculums for all the isolates were prepared by taking a loopful from the prepared plate in the Nutrient broth and keeping it in the incubator overnight at 37 °C. The next day, the growth was observed [27] …”

Section: Methodsmentioning

confidence: 99%

Synthesis of 1,2,4‐Oxadiazole‐sulfonamide Conjugates and Their Synergistic Effect with Ampicillin as Antimicrobial Agents

Habib,

Ali,

Irfan

et al. 2023

ChemistrySelect

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In this investigation, we synthesized substituted 1,2,4‐oxadiazole‐sulfonamide conjugates (8 a–8 l) through a multistep synthetic approach. These compounds were then evaluated against six bacterial strains, consisting of three Gram‐positive and three Gram‐negative strains. Among them, compound 8 d displayed notable inhibitory activity, with minimum inhibitory concentration (MIC) values of 64 μg/mL observed against the Gram‐positive bacteria Staphylococcus aureus and Enterococcus faecalis. Notably, in the disk diffusion assay, compound 8 d exhibited the most substantial zone of inhibition (ZOI) against Escherichia coli and S. aureus, with respective ZOI measurements of 14 mm and 15 mm, both at a concentration of 2MIC. Additionally, supplementary investigations, including the Fractional Inhibitory Concentration Index (FICI) provided further support for the inhibitory potential of 8 d against the standard bacterial strains. When combined with Ampicillin (Amp), it demonstrated a synergistic effect, significantly augmenting its antibacterial activity against Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterococcus faecalis. Importantly, it exhibited no toxicity toward human red blood cells (hRBC). Furthermore, The Absorption, Distribution, Metabolism, and Excretion (ADME) profile of 8 d indicated its favorable drug‐like characteristics.

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Section: Methodsmentioning

confidence: 99%

Synthesis of 1,2,4‐Oxadiazole‐sulfonamide Conjugates and Their Synergistic Effect with Ampicillin as Antimicrobial Agents

Habib,

Ali,

Irfan

et al. 2023

ChemistrySelect

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“…Abass et al [37] found that extracts of B. aristata had antibacterial activity against E. coli and S. aureus. The authors achieved good results thereby concluding that the extracts of the plant could be utilized as a capable source of natural antibacterial compounds.…”

Section: Antibacterial Potentialmentioning

confidence: 99%

“…Studies have shown that B. aristata has significant antioxidant activity due to its capability to scavenge free radicals, reduce oxidative stress, and protect cells from damage caused by toxins. The plant is a good source of phenolic compounds, which are known to possess strong antioxidant properties [37]. These compounds are able to scavenge free radicals and inhibit the production of ROS.…”

Section: Antioxidant Activitymentioning

confidence: 99%

The medicinal plant Berberis aristata and its endophytes for pharmacological applications: Current research and future challenges

Sharma,

Chaitanya,

Sharma

et al. 2024

J App Biol Biotech

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Exploring medicinal plants for their natural products to benefit humanity has been a practice since ancient times. Berberis aristata, also known as tree turmeric or Indian barberry, has a dominant spot in conventional medicine for 2500 plus years. In the latter years, this particular species has gained prime attention in pharmaceutical zone due to its scarcity. The plant and its formulations are used to treat numerous human health threats including cancer, diabetes, diarrhea, wound relieving, infections of eyes and ears, gynecological disorders, and HIV-AIDS. The bioactive compounds present in B. aristata are found to contain anti-inflammatory, antioxidant, and anti-microbial properties. The plant is critically jeopardized species of Indian Himalaya due to the collection of its roots at a large scale for obtaining berberine alkaloid and its conservation is of great concern. At present, isolation of endophytic entities from remedial plants revolutionized the path of discovering drugs from medicinal plants. Endophytes can produce the same bioactive as their host plant as well as another bioactive to address a variety of human health issues. The review examines the bioprospecting of B. aristata, a medicinal shrub from the family Berberidaceae, and its associated endophytes for the production of bioactive compounds with application to human health. Finally, the review provides a comprehensive outlook on the current status and future prospects of bioprospecting B. aristata and its endophytes to combat future human health threats is an exciting area of research that should be further explored.

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“…Andrographolide is a diterpenoid class of secondary metabolites that mainly accumulates in the leaves of the plant and is synthesized by the mevalonate (MVA) and methylerythritol 4-phosphate (MEP) pathway [5]. The various important pharmacological effects, such as immunostimulatory, anticancer, anti-hepatitis, cardiovascular, anti-HIV, anti-inflammatory, anti-microbial, antimalarial, cytotoxic, hepatoprotective, antioxidant, and antidiarrheal, of A. paniculata are well documented [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Therefore, it has been listed as an essential medicinal plant species by the Indian National Medicinal Plants Board [22].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production

Kumar,

Roy Choudhury

et al. 2024

Genes

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Andrographis paniculata (Burm. f.) Nees is an important medicinal plant known for its bioactive compound andrographolide. NAC transcription factors (NAM, ATAF1/2, and CUC2) play a crucial role in secondary metabolite production, stress responses, and plant development through hormonal signaling. In this study, a putative partial transcript of three NAC family genes (ApNAC83, ApNAC21 22 and ApNAC02) was used to isolate full length genes using RACE. Bioinformatics analyses such as protein structure prediction, cis-acting regulatory elements, and gene ontology analysis were performed. Based on in silico predictions, the diterpenoid profiling of the plant’s leaves (five-week-old) and the real-time PCR-based expression analysis of isolated NAC genes under abscisic acid (ABA) treatment were performed. Additionally, the expression analysis of isolated NAC genes under MeJA treatment and transient expression in Nicotiana tabacum was performed. Full-length sequences of three members of the NAC transcription factor family, ApNAC83 (1102 bp), ApNAC21 22 (996 bp), and ApNAC02 (1011 bp), were isolated and subjected to the promoter and gene ontology analysis, which indicated their role in transcriptional regulation, DNA binding, ABA-activated signaling, and stress management. It was observed that ABA treatment leads to a higher accumulation of andrographolide and 14-deoxyandrographolide content, along with the upregulation of ApNAC02 (9.6-fold) and the downregulation of ApNAC83 and ApNAC21 22 in the leaves. With methyl jasmonate treatment, ApNAC21 22 expression decreased, while ApNAC02 increased (1.9-fold), with no significant change being observed in ApNAC83. The transient expression of the isolated NAC genes in a heterologous system (Nicotiana benthamiana) demonstrated their functional transcriptional activity, leading to the upregulation of the NtHMGR gene, which is related to the terpene pathway in tobacco. The expression analysis and heterologous expression of ApNAC21 22 and ApNAC02 indicated their role in andrographolide biosynthesis.

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Development of Synergy-Based Combination of Methanolic Extract of Andrographis paniculata and Berberis aristata Against E. coli and S. aureus

Cited by 14 publications

References 44 publications

Synthesis of 1,2,4‐Oxadiazole‐sulfonamide Conjugates and Their Synergistic Effect with Ampicillin as Antimicrobial Agents

Synthesis of 1,2,4‐Oxadiazole‐sulfonamide Conjugates and Their Synergistic Effect with Ampicillin as Antimicrobial Agents

The medicinal plant Berberis aristata and its endophytes for pharmacological applications: Current research and future challenges

Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production

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