Expanding the assortment of lactose-free dairy products, namely, ice cream for people who cannot tolerate lactose is a promising direction of research. The purpose of the work is to develop the technology of milk lactose-free synbiotic yogurt ice cream based on buttermilk protein-lipid concentrate, obtained by ultrafiltration/diafiltration, with the addition of natural flavoring and food additives, and the study of its quality indicators. A protein-lipid concentrate of buttermilk with a low lactose content was obtained by ultrafiltration/diafiltration, and its quality indicators were determined. The concentrate has a high protein content (16.01%) and a low lactose content (1.28%). Protein-lipid concentrate fermented with synbiotic sourdough is the main component included in the formulation of lactose-free synbiotic yogurt ice cream. The main physico-chemical parameters of the obtained fermented protein-lipid concentrate were determined, which ensures a rational content of the main components of the chemical composition in the finished lactose-free ice cream (it does not contain lactose, the protein content is 8.46%) and a high content of useful microflora (bifido- and lactobacilli). A mixture for lactose-free synbiotic yogurt ice cream was obtained according to a scientifically based recipe. A sample of lactose-free ice cream was made from the mixture using traditional technology, and organoleptic, physico-chemical and microbiological quality indicators were investigated. A comparison of the quality indicators of the studied sample with the control, which was taken as classic yogurt ice cream, was carried out. The mass fraction of lactose in samples of lactose-free ice cream is 0,011%. The developed lactose-free synbiotic yogurt ice cream has high probiotic activity (contains lacto- (3.0•108 CFU/g) and bifidobacteria (3.5•109 CFU/g)).