Development of tensile strength methodology for murine skin wound healing

Bellare, Anuj; Epperly, Michael W.; Greenberger, Joel S.; Fisher, Renee; Glowacki, Julie

doi:10.1016/j.mex.2018.04.002

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…The healing of the primary-closed incisions was estimated through the tensile strength test. Among the variety of biomechanical methods determining the extent of skin wound recovery, the assay mentioned above stood out as one of the easiest for implementation [59].…”

Section: In Vivo Wound-healing Effectmentioning

confidence: 99%

Bigel Formulations of Nanoencapsulated St. John’s Wort Extract—An Approach for Enhanced Wound Healing

Sotirova

Gugleva

Stoeva

et al. 2023

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This study aimed to develop a semisolid vehicle for topical delivery of nanoencapsulated St. John’s wort (SJW) extract, rich in hyperforin (HP), and explore its wound-healing potential. Four nanostructured lipid carriers (NLCs) were obtained: blank and HP-rich SJW extract-loaded (HP-NLC). They comprised glyceryl behenate (GB) as a solid lipid, almond oil (AO), or borage oil (BO) representing the liquid lipid, along with polyoxyethylene (20) sorbitan monooleate (PSMO) and sorbitan monooleate (SMO) as surfactants. The dispersions demonstrated anisometric nanoscale particles with acceptable size distribution and disrupted crystalline structure, providing entrapment capacity higher than 70%. The carrier exhibiting preferable characteristics (HP-NLC2) was gelled with Poloxamer 407 (PM407) to serve as the hydrophilic phase of a bigel, to which the combination of BO and sorbitan monostearate (SMS) organogel was added. The eight prepared bigels with different proportions (blank and nanodispersion-loaded) were characterized rheologically and texturally to investigate the impact of the hydrogel-to-oleogel ratio. The therapeutic potential of the superior formulation (HP-NLC-BG2) was evaluated in vivo on Wistar male rats through the tensile strength test on a primary-closed incised wound. Compared with a commercial herbal semisolid and a control group, the highest tear resistance (7.764 ± 0.13 N) was achieved by HP-NLC-BG2, proving its outstanding wound-healing effect.

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Section: In Vivo Wound-healing Effectmentioning

confidence: 99%

Bigel Formulations of Nanoencapsulated St. John’s Wort Extract—An Approach for Enhanced Wound Healing

Sotirova

Gugleva

Stoeva

et al. 2023

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“…Half of the mice in the 0, 8, or 18 Gy plus incision groups were sacrificed at 30 d or at 60 d after irradiation. The tensile strength was determined across the incisions with skin from mice in each group along with control mice (no irradiation and no incision) [24].…”

Section: Local Irradiation and Incisional Skin Woundmentioning

confidence: 99%

“…In addition to the genotypic differences, pilot studies revealed a lack of feasibility to handle and process large numbers of fresh samples with a standard tensiometer. To solve this limitation, elements of a mouse skin injury model and of tools and a protocol for measurement of tensile strength were modified and validated for reproducibility [24].…”

Section: Effects Of Local or Tbi On Incisional Skin Woundsmentioning

confidence: 99%

Combined injury: irradiation with skin or bone wounds in rodent models

Glowacki¹,

Epperly²,

Bellare³

et al. 2021

J. Radiol. Prot.

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A radiation combined injury is defined as an injury that occurs in the setting of irradiation, such as those expected after a nuclear accident, radiation dispersal device release (a ‘dirty bomb’), or a nuclear weapon detonation. There is much research on irradiation-associated burns and their healing, but there is less known about other injuries sustained in the context of irradiation. Animal models are limited in their correlations to clinical situations but can support research on specific questions about injuries and their healing. Mouse models of irradiation with skin or bone wounds are validated as highly reproducible and quantitative. They show dose-dependent impairment of wound healing, with later recovery. Irradiation-induced delay of bone wound healing was mitigated to different extents by single doses of gramicidin S-nitroxide JP4-039, a plasmid expressing manganese superoxide dismutase, amifostine/WR2721, or the bifunctional sulfoxide MMS-350. These models should be useful for research on mechanisms of radiation dermal and osseous damage and for further development of new radioprotectors. They also provide information of potential relevance to the effects of clinical radiation therapies.

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“…Luminol is a relatively new means of quantifying reactive oxygen species (ROS) in murine wound models, 18 and differences in wound ROS with vs without NIR‐PIT would suggest a potential negative impact on wound healing. Direct measurement of wound tensile strength is another validated method of ascertaining interference in wound healing in murine models 19 . Thus, using these methods, we investigated the effects of perioperative NIR‐PIT on postoperative wound healing in a murine model of cancer.…”

Section: Introductionmentioning

confidence: 99%

“…Direct measurement of wound tensile strength is another validated method of ascertaining interference in wound healing in murine models. 19 Thus, using these methods, we investigated the effects of perioperative NIR-PIT on postoperative wound healing in a murine model of cancer.…”

mentioning

confidence: 99%

Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy

et al. 2020

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Near‐infrared photoimmunotherapy (NIR‐PIT) is a novel cancer therapy that employs a combination of infrared light and tumor‐targeted monoclonal antibody‐photoabsorber conjugates to cause both direct tumor necrosis and immunogenic cell death. NIR‐PIT may have potential in the perioperative setting before surgery, and therefore it is important to know the effect of NIR‐PIT on wound healing. Fifty mice were implanted with subcutaneous xenografts of N87 human gastric cancer cells, and tumors were excised after reaching a predetermined size. After excision, 30 mice were split into three groups: Controls, NIR‐PIT 1 day prior to surgery and NIR‐PIT 3 days prior to surgery. The quantity of reactive oxygen species (ROS) in each wound was measured on Postoperative Days 2 and 4, and mice were monitored weekly for 4 weeks for evidence of local tumor recurrence as well as clinical evidence of wound healing complications (eg, dehiscence, infection). The remaining 20 mice (10 controls, 10 treated with NIR‐PIT 1 day prior to surgery) were sacrificed on either Postoperative Day 7 or 14, the skin around wounds were excised, and tensile strength was measured with a digital force gauge. There were no significant differences between treatment and control groups with respect to wound ROS levels, wound tensile strength, local tumor recurrence, or postoperative complication rates ( P > .05). In conclusion, neoadjuvant (pre‐operative) NIR‐PIT shows no evidence of adverse wound healing effects, and it is likely a safe adjunctive treatment to surgery. Postoperative use of NIR‐PIT merits investigation.

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Development of tensile strength methodology for murine skin wound healing

Abstract: Graphical abstract

Cited by 7 publications

References 24 publications

Bigel Formulations of Nanoencapsulated St. John’s Wort Extract—An Approach for Enhanced Wound Healing

Bigel Formulations of Nanoencapsulated St. John’s Wort Extract—An Approach for Enhanced Wound Healing

Combined injury: irradiation with skin or bone wounds in rodent models

Wound healing after excision of subcutaneous tumors treated with near‐infrared photoimmunotherapy

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