Development of tetraploid plants from an interspecific hybrid between mungbean (Vigna radiata) and rice bean (Vigna umbellata)

Chaisan, Tanapon; Somta, Prakit; Srinives, Peerasak; Chanprame, Sontichai; Kaveeta, R.; Dumrongkittikule, Surapong

doi:10.1007/s12892-012-0078-y

Cited by 15 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous research publications have indicated that colchicine is the most common mitosis inhibitor used for the production of polyploids of legumes (Anderson et al, 1991;Algan, Buyukkartal, 2000;Wang et al, 2009;Wu et al, 2010;Chaisan et al, 2013). One µM colchicine (24 h) was best suited for Brassica napus L. polyploidization.…”

Section: Resultsmentioning

confidence: 99%

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

Dabkevičienė

Statkevičiūtė

Mikaliūnienė

et al. 2016

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

The current study was set out to establish the efficacy of different mitosis inhibitors (colchicine, amiprophos-methyl, trifluralin and oryzalin) used in the production of red clover (Trifolium pratense L.) and alsike clover (Trifolium hybridum L.) polyploids and to assess the agrobiological traits of tetraploid populations. Four cultivars and five wild populations of T. pratense, one cultivar and three wild populations of T. hybridum were used for polyploidization. The effect of conventional soaking of germinating seeds in a colchicine solution was compared with that of colchicine treatment of embryos in an in vitro culture. Colchicine treatment of T. pratense embryos resulted in 3.3 times higher production of tetraploids (teraploid yield 55.0%) compared with colchicine treatment of seedlings, and the production of chimeric plants was 1.9 times lower. The different mitosis inhibitors were characterised by a diverse polyploidization effect. Tetraploid yield depended not only on the mitosis inhibitor used but also on the plant species. Treatment of T. pratense embryos with colchicine, amiprophosmethyl and oryzalin yielded similar results in terms of tetraploid production (31.3-40.7%) and chimeras (14.3-22.4%). The concentration of trifluralin used for the production of T. pratense tetraploids was ineffective. Colchicine solution was more efficient for the development of T. hybridum tetraploids whose yield was 2.5 times as high as that obtained having treated the embryos with amiprophos-methyl. Induced tetraploid populations were compared to reference cultivars in field trials. The two T. pratense populations 'Radviliai 4n' and 'Arimaičiai 4n' stood out in this respect -their plants accumulated higher dry matter contents, produced more inflorescences than those of the reference cultivar and exhibited prolific seed yield capacity. In relation to the agrobiological traits, the tetraploid populations of T. hybridum did not surpass the reference cultivar.

show abstract

Section: Resultsmentioning

confidence: 99%

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

Dabkevičienė

Statkevičiūtė

Mikaliūnienė

et al. 2016

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

show abstract

“…More recently, Chaisan et al. () obtained interspecific hybrids between V. radiata (cv. ‘Kamphaeng Saen 2’) × V. umbellata (cv.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of sources for photo‐ and thermo‐insensitivity in Vigna species

et al. 2014

View full text Add to dashboard Cite

Cultivation of the same varieties of mungbean and blackgram across different seasons and locations is constrained by their photo-and thermosensitive behaviour. Developing insensitive genotypes, which can fit well across all seasons, requires robust donors which would provide genes imparting this trait. This study was undertaken to identify such donors in the Vigna species. Forty-eight accessions belonging to 13 Vigna species and eight released cultivars were evaluated under natural field conditions. Among these, two accessions, viz. V. umbellata (IC251442) and V. glabrescens (IC251372) were found photo-and thermo-insensitive as these were able to flower and set pods at temperatures as high as 43.9°C and as low as 2.7°C. Pollen viability studies indicated viable pollen (>75% at 2.7°C and >85% at 41.9°C) and normal pollen tube growth at both the extremes of temperature. The identified V. glabrescens accession has long, constricted pods and dark green, mottled seeds while V. umbellata has smooth, curved pods and shining, oval, large seeds. Both these accessions can be utilized in developing photo-thermo insensitive genotypes in cultivated Vigna species.Key words: photo -thermo insensitivity -V. umbellata -V. glabrescens -wild VignaThe genus Vigna comprises more than 200 immensely variable species. Many of these are agronomically important and depict considerable economic and environmental importance. Amongare cultivated and consumed mostly in south and Southeast Asia and Africa. Many of the above species beside their high protein grains are valued as forage, green manure and cover crops. Mungbean and blackgram are the most popular and economically important crops of the Vigna group. India is the largest producer of these crops in the world and accounts for 65% of acreage and 54% of global production (Pratap et al. 2012). Keeping in view their short crop cycle, low input requirements and ability to survive in a wide range of adverse soil and climatic conditions, there is an ample scope of growing these crops across the seasons (spring, summer, winter and rainy seasons) in different parts of the country as a sole, relay or intercrop (Pratap et al. 2013). There is also a large scope of their further horizontal expansion in large areas, particularly in the rice fallows in peninsular India and in summer cultivation after the harvest of wheat, potato and rapeseed-mustard in Punjab, Haryana, Rajasthan and western Uttar Pradesh. However, the biggest hindrance in horizontal expansion of blackgram and mungbean in India is their photo-and thermo-sensitive nature which necessitates breeding of an array of genotypes for different agro-climatic zones of the country. Most of the varieties of mungbean and blackgram which have been bred till date are suitable only for a particular agro-climatic zone and cropping season. Nevertheless, most of the farmers usually recycle their seeds year after year and also across different seasons being unaware about the suitability of a particular variety in a specific season. In most of the...

show abstract

“…Similarly, ploidy level was also not reported to affect crossability as crosses were obtained successfully between diploid × tetraploid ( V. radiata × V. glabrescens ) (Chen et al 1989 ;Krishnan and De 1968 ) and tetraploid × diploid ( V. glabrescens × V. umbellata ) Vigna species. Postfertilization barriers of varying degrees have also been reported in most of the interspecifi c Vigna crosses (Dana 1964 ;Biswas and Dana 1975 ;Machado et al 1982 ;Chen et al 1983 ;Al-Yasiri and Coyne 1966 ;Bharathi et al 2006 ;Pandiyan et al 2010 ;Chaisan et al 2013 ). These affect the successful recovery of desirable recombinants through development of shriveled hybrid seed with reduced or no germination (hybrid inviability), development of dwarf and non-vigorous plants and death of F 1 plants at critical stages of development (hybrid lethality).…”

Section: Crossability Barriersmentioning

confidence: 92%

“…Singh et al ( 2003 ) also produced successful hybrids between V. radiata and V. umbellata , and the hybrids possessed intermediate morphology with MYMV resistance. Recently, Chaisan et al ( 2013 ) successfully obtained interspecifi c hybrids between V. radiata (cv. Kamphaeng Saen 2 ) and V. umbellata (cv.…”

Section: Embryo Rescuementioning

confidence: 99%

“…Using this technique, successful crosses have been attempted between V. radiata × V. mungo (Pande et al 1990 ) and V. radiata × V. trilobata (Dana 1966 ). In the study by Chaisan et al ( 2013 ), the hybrid sterility problem between the interspecifi c hybrids obtained from the cross V. radiata (cv. "Kamphaeng Saen 2) × V. umbellata (cv.…”

Section: Polyploidizationmentioning

confidence: 99%

See 1 more Smart Citation

Vigna

Pratap

Malviya

Tomar

et al. 2013

Alien Gene Transfer in Crop Plants, Volume 2

View full text Add to dashboard Cite

The genus Vigna comprises more than 200 species of which 7 are of tremendous agronomic importance. These are grown mainly in the warm temperate and tropical regions of the world. Valued for their grains with high and easily digestible proteins, these crops are also known as forage, green manure, and cover crops. Due to a short life cycle, these are suitable as catch crops and also fi t well in intercropping, mixed or relay cropping. For genetic improvement of cultivated vignas, mainly cultivated germplasm and exotic lines have been used so far. However, despite development of several improved cultivars in different Vigna crops, biotic and abiotic stresses still remain the major constraints in realizing their true yield potential. While plethora of genes conferring resistance/tolerance to these stresses have already been transferred from cultivated germplasm, wild genetic resources offer additional sources of useful alien variation which can be incorporated in cultivated Vigna through alien gene transfer. With better understanding of the processes behind pollen germination and pollen tube growth, fertilization, embryo and endosperm development and inheritance pattern, strategies have been developed to avoid pre-and post-fertilization barriers in successful distant hybridization leading to alien gene transfer. These include making reciprocal crosses, repeated pollination, hormonal treatment of fl ower buds, polyploidization, use of bridge species and most importantly, embryo rescue which have increased success rate of alien gene transfer in Vigna through sexual hybridization. Nevertheless, alien gene transfer through genetic transformation and use of molecular breeding tools still lag behind in Vigna and therefore need special attention. The signifi cant achievements made in different Vigna species in alien gene transfer and their utilization have been discussed in this chapter.

show abstract

Development of tetraploid plants from an interspecific hybrid between mungbean (Vigna radiata) and rice bean (Vigna umbellata)

Cited by 15 publications

References 28 publications

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

Identification and characterization of sources for photo‐ and thermo‐insensitivity in Vigna species

Vigna

Contact Info

Product

Resources

About