Development of the electrochemical, spectroscopic and molecular docking approaches toward the investigation of interaction between DNA and anti-leukemic drug azacytidine

Nimal, Rafia; Ünal, Didem Nur; Erkmen, Cem; Bozal‐Palabiyik, Burcin; Siddiq, Muhammad; Eren, Gökçen; Shah, Afzal; Uslu, Bengi

doi:10.1016/j.bioelechem.2022.108135

Cited by 15 publications

(11 citation statements)

References 53 publications

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“…Specific DNA–DOX (and DOX nanoderivatives) interactions produce registered changes of the electron transfer on the electrode interface and, as a result, the opportunity for assessing thermodynamic parameters, such as affinity constants and changes in Gibbs energy (ΔG) [ 53 , 54 ]. Electrochemical methods for DNA analysis and drug–DNA interaction possess high sensitivity and multiparametric assays for the calculation of thermodynamic parameters.…”

Section: Resultsmentioning

confidence: 99%

Interaction of Doxorubicin Embedded into Phospholipid Nanoparticles and Targeted Peptide-Modified Phospholipid Nanoparticles with DNA

et al. 2023

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The interactions of dsDNA with new targeted drug delivery derivatives of doxorubicin (DOX), such as DOX embedded into phospholipid nanoparticles (NPhs) and DOX with the NGR targeted peptide-modified NPhs were studied electrochemically by differential pulse voltammetry technique. Screen-printed electrodes (SPEs), modified with stable fine dispersions of carbon nanotubes (CNTs), were used for quantitative electrochemical investigations of direct electrochemical oxidation of guanine, adenine, and thymine heterocyclic bases of dsDNA, and their changes in the presence of DOX nanoderivatives. Analysing the shifts of peak potentials of nucleobases in the presence of drug, we have shown that the doxorubicin with NGR targeted peptide changed the mode of interaction in DNA–drug complexes from intercalative to electrostatic. Binding constants (Kb) of DNA–drug complexes were calculated in accordance with adenine, guanine, and thymine oxidation signals. Based on our experiments, we have proven that the surface modification of a drug delivery system with NGR targeted peptide dramatically changed the mechanism of interaction of drug with genetic material. DNA-mediated drug toxicity was calculated based on the concentration-dependent “response” of heterocyclic nucleobases on drug influence. DOX, DOX-loaded phospholipid nanoparticles (NPhs), and DOX with NGR addressed peptide-modified NPhs were moderately toxic in the concentration range of 0.5–290 µM.

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Section: Resultsmentioning

confidence: 99%

Interaction of Doxorubicin Embedded into Phospholipid Nanoparticles and Targeted Peptide-Modified Phospholipid Nanoparticles with DNA

et al. 2023

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“…27 Biosensor fabrication.-A biosensor consisting of three layers was created by applying a 5 μl coating of 50 ppm ct-dsDNA over the surface of a GCE. [28][29][30][31] Following the complete drying of the surface, this technique was repeated three more times. Once a drop was applied to the electrode surface, the ct-dsDNA coating was left undisturbed until it had dried.…”

Section: Methodsmentioning

confidence: 99%

Electrochemical Investigations and Molecular Docking Analysis to Evaluate the Molnupiravir-Calf Thymus dsDNA Interaction

Kucuk,

Unal,

Karayel

et al. 2024

J. Electrochem. Soc.

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Molnupiravir (MLP) is an important antiviral drug recommended for the treatment of COVID-19. In order to design new pharmaceuticals, exploring drug and DNA interaction is crucial . This study aimed to determine the interaction of MLP with calf thymus double-stranded DNA (ct-dsDNA) by electrochemical methods. Investigation of these interactions was carried out using the differential pulse voltammetry technique (DPV) on the biosensor surface and in-solution studies. Changes in ct-dsDNA between deoxyguanosine (dGuo) and deoxyadenosine (dAdo) oxidation signals were examined before and after the interaction. It was found that MLP interacts significantly with bases of ct-dsDNA dAdo. Limits of detection and quantification for MLP-ct-dsDNA interaction were calculated as 2.93 and 9.67 µM in the linear range of 10-200 µM, respectively, based on dAdo's decreasing peak current. To calculate the binding constant of MLP and ct-dsDNA, cyclic voltammetry was used, and it was found to be 8.6×104 M. As for molecular docking techniques, the binding energy of MLP with DNA is −8.1 kcal/mol, and this binding occurred by a combination of strong conventional hydrogen bonding to both adenine and guanine base pair edges, which indicates the interaction of MLP with DNA.

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“…In a recent study by Nimal et al, an electrochemical biosensor was developed for monitoring the DNA interaction with the antileukemia drug azacytidine [ 32 ]. A GCE was used in the study and the drug–DNA interaction was investigated through the change in guanine and adenine signals measured by DPV.…”

Section: Electrochemical Detection Of Drug–dna Interactionsmentioning

confidence: 99%

Electrochemical DNA biosensors developed for the monitoring of biointeractions with drugs: a review

GÜRSAN,

ŞENTÜRK,

GÖZ

et al. 2023

Turkish Journal of Chemistry

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The interaction of drugs with DNA is important for the discovery of novel drug molecules and for understanding the therapeutic effects of drugs as well as the monitoring of side effects. For this reason, many studies have been carried out to investigate the interactions of drugs with nucleic acids. In recent years, a large number of studies have been performed to electrochemically detect drug–DNA interactions. The fast, sensitive, and accurate results of electrochemical techniques have resulted in a leading role for their implementation in this field. By means of electrochemical techniques, it is possible not only to demonstrate drug–DNA interactions but also to quantitatively analyze drugs. In this context, electrochemical biosensors for drug–DNA interactions have been examined under different headings including anticancer, antiviral, antibiotic, and central nervous system drugs as well as DNA-targeted drugs. An overview of the studies related to electrochemical DNA biosensors developed for the detection of drug–DNA interactions that were reported in the last two decades in the literature is presented herein along with their applications and they are discussed together with their future perspectives.

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Development of the electrochemical, spectroscopic and molecular docking approaches toward the investigation of interaction between DNA and anti-leukemic drug azacytidine

Cited by 15 publications

References 53 publications

Interaction of Doxorubicin Embedded into Phospholipid Nanoparticles and Targeted Peptide-Modified Phospholipid Nanoparticles with DNA

Interaction of Doxorubicin Embedded into Phospholipid Nanoparticles and Targeted Peptide-Modified Phospholipid Nanoparticles with DNA

Electrochemical Investigations and Molecular Docking Analysis to Evaluate the Molnupiravir-Calf Thymus dsDNA Interaction

Electrochemical DNA biosensors developed for the monitoring of biointeractions with drugs: a review

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