Development of the Functional Male Sterile Line of Eggplant Utilizing the Cytoplasm of Solanum kurzii by Way of the Amphidiploid

Khan, Md. Mizanur Rahim; Takashi, Arita; Iwayoshi, Masaki; Ogura‐Tsujita, Yuki; Isshiki, Shiro

doi:10.2525/ecb.58.79

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This interspecific crossing dragged the maternal cytoplasmic background of the wild parent, which might have caused a partial sterility and bias in subsequent generations. Some studies confirmed a strong effect of wild Solanum cytoplasms in the reduction in pollen fertility of alloplasmic lines (Khan et al, 2020;Isshiki et al, 2021). However, the PCA highlighted the absence of a population structure, also confirmed by the lack of genetically differentiated cytoplasmic groups.…”

Section: Discussionmentioning

confidence: 96%

Newly Developed MAGIC Population Allows Identification of Strong Associations and Candidate Genes for Anthocyanin Pigmentation in Eggplant

et al. 2022

View full text Add to dashboard Cite

Multi-parent advanced generation inter-cross (MAGIC) populations facilitate the genetic dissection of complex quantitative traits in plants and are valuable breeding materials. We report the development of the first eggplant MAGIC population (S3 Magic EGGplant InCanum, S3MEGGIC; 8-way), constituted by the 420 S3 individuals developed from the intercrossing of seven cultivated eggplant (Solanum melongena) and one wild relative (S. incanum) parents. The S3MEGGIC recombinant population was genotyped with the eggplant 5k probes SPET platform and phenotyped for anthocyanin presence in vegetative plant tissues (PA) and fruit epidermis (FA), and for the light-insensitive anthocyanic pigmentation under the calyx (PUC). The 7,724 filtered high-confidence single-nucleotide polymorphisms (SNPs) confirmed a low residual heterozygosity (6.87%), a lack of genetic structure in the S3MEGGIC population, and no differentiation among subpopulations carrying a cultivated or wild cytoplasm. Inference of haplotype blocks of the nuclear genome revealed an unbalanced representation of the founder genomes, suggesting a cryptic selection in favour or against specific parental genomes. Genome-wide association study (GWAS) analysis for PA, FA, and PUC detected strong associations with two myeloblastosis (MYB) genes similar to MYB113 involved in the anthocyanin biosynthesis pathway, and with a COP1 gene which encodes for a photo-regulatory protein and may be responsible for the PUC trait. Evidence was found of a duplication of an ancestral MYB113 gene with a translocation from chromosome 10 to chromosome 1 compared with the tomato genome. Parental genotypes for the three genes were in agreement with the identification of the candidate genes performed in the S3MEGGIC population. Our new eggplant MAGIC population is the largest recombinant population in eggplant and is a powerful tool for eggplant genetics and breeding studies.

show abstract

Section: Discussionmentioning

confidence: 96%

Newly Developed MAGIC Population Allows Identification of Strong Associations and Candidate Genes for Anthocyanin Pigmentation in Eggplant

et al. 2022

View full text Add to dashboard Cite

show abstract

“…This variability can manifest in both quantitative and qualitative traits that are valuable for breeding prior to the commencement of the crossbreeding program [2]. Although the sexual interspecific hybrids between S. melongena and S. aethiopicum are highly sterile, it has been demonstrated that recombination between these two genomes is possible, and that backcrossing hybrid generations from S. melongena with useful introgressions from the S. aethiopicum Gilo group can occur [11].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the productivity potential of eggplant lines developed on the basis of interspecific hybridisation and gametic breeding

Kondratenko,

Samovol,

Serhiienko

et al. 2024

PVSandP

View full text Add to dashboard Cite

Purpose. Improvement of the gene pool of the cultivated form of eggplant (Solanum melongena L.) through interspecific hybridisation with Solanum aethiopicum L. and gametic breeding; analysis of the interspecific lines for a set of valuable quantitative traits determining the yield structure. Methods. Studies for the comprehensive evaluation of eggplant lines of interspecific origin were carried out during 2021-2023 in protected soil conditions at the experimental base of the Institute for Vegetable and Melon Growing of the National Academy of Agrarian Sciences. Phenological observations and biometric measurements of plants of four lines were carried out. The stability of the manifestation of seven quantitative traits during the research years was determined by the methods of variation statistics (calculation of the mean square deviation (σ), coefficient of variation (V), Pearson's paired linear correlation (rp)). Results. The analysis identified eggplant lines of interspecific origin that were superior to the standard variety 'Almaz' in terms of the level and stability of quantitative traits. Thus, F6(Pavlotas-20/var. 'Almaz')I1 and ВС1[F5 (Pavlotas-20/var. 'Almaz')]I1 showed a statistically significant increase in fruit width (by 16.48% and 14.85%, respectively). Meanwhile, line BC2 [F5 (Pavlotas-20/var. 'Almaz')]I1 exhibited higher productivity (by 18.11%) compared to the standard variety. The correlation analysis shows that the trait “Productivity of one plant” has a strong positive relationship with the trait “Number of fruits per plant” (rp = 0.75) and a strong negative relationship with “Average fruit width” (rp = -0.70) and “Duration of the period from mass germination to technical fruit maturity” (rp = -0.72). The selected line BC2[F5(Pavlotas-20 / var. 'Almaz')]I1 exhibited a four-day shorter period from mass germination to technical fruit maturity compared to the standard variety. Conclusions. The line BC2[F5(Pavlotas-20 / var. ' Almaz ')]I1was isolated based on the complex of quantitative traits. It underwent an additional stage of gametophytic breeding, resulting in a high adaptive potential to growing conditions.

show abstract

Newly developed MAGIC population allows identification of strong associations and candidate genes for anthocyanin pigmentation in eggplant

Mangino

Arrones

Plazas

et al. 2021

Preprint

View full text Add to dashboard Cite

MAGIC populations facilitate the genetic dissection of complex quantitative traits in plants and are valuable breeding materials. We report the development of the first eggplant MAGIC population (S3MEGGIC; 8-way), constituted by 420 S3 individuals developed from the intercrossing of seven cultivated eggplant (Solanum melongena) and one wild relative (S. incanum) parents. The S3MEGGIC recombinant population was genotyped with the eggplant 5k probes SPET platform and phenotyped for anthocyanins presence in vegetative plant tissues (PA) and fruit epidermis (FA), and for the light-sensitive anthocyanic pigmentation under the calyx (PUC). The 7,724 filtered high-confidence SNPs confirmed a low residual heterozygosity (6.87%) and a lack of genetic structure in the S3MEGGIC population, including no differentiation among subpopulations carrying cultivated or wild cytoplasm. Inference of haplotype blocks of the nuclear genome revealed an unbalanced representation of founder genomes, suggesting cryptic selection in favour or against specific parental genomes. GWAS analysis for PA, FA and PUC detected strong associations with two MYB genes similar to MYB113 involved in the anthocyanin biosynthesis pathway and with a COP1 gene, which encodes for a photo-regulatory protein and may be responsible for the PUC phenotype. Evidence was found of a duplication of an ancestral MYB113 gene with a translocation from chromosome 10 to chromosome 1. Parental genotypes for the three genes were in agreement with the candidate genes identification performed in the S3MEGGIC population. Our new eggplant MAGIC population is the largest recombinant population in eggplant and is a powerful tool for eggplant genetics and breeding studies.

show abstract

Development of the Functional Male Sterile Line of Eggplant Utilizing the Cytoplasm of Solanum kurzii by Way of the Amphidiploid

Cited by 3 publications

References 13 publications

Newly Developed MAGIC Population Allows Identification of Strong Associations and Candidate Genes for Anthocyanin Pigmentation in Eggplant

Newly Developed MAGIC Population Allows Identification of Strong Associations and Candidate Genes for Anthocyanin Pigmentation in Eggplant

Evaluation of the productivity potential of eggplant lines developed on the basis of interspecific hybridisation and gametic breeding

Newly developed MAGIC population allows identification of strong associations and candidate genes for anthocyanin pigmentation in eggplant

Contact Info

Product

Resources

About