Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA

Suzuki, Ryota; Yoshikawa, Tetsushi; Ihira, Masaru; Enomoto, Yoshihiko; Inagaki, Shoji; Matsumoto, Koichí; Kato, Koji; Kudo, Kazuko; Kojima, Seiji; Asano, Yoshizo

doi:10.1016/j.jviromet.2005.09.008

Cited by 34 publications

(25 citation statements)

References 25 publications

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“…Based on the FLEP (5-FU, leucovorin, etoposide, and cisplatin) regimen. After the exploring of the preoperative chemotherapy which combines arterial with venous administration since December 2002 [9,10] we obtained a very satisfactory efficacy rate with the FLEEOX regimen for unresectable, advanced gastric cancer. The FLEEOX regimen is a combination of venous and regional chemotherapy, comprising continuous intravenous infusion 5-fluorouracil and leucovorin, followed by intra-aterial infusion of epirubicin, etoposide, and oxaliplatin.…”

Section: Introductionmentioning

confidence: 99%

Comparison of efficacy of different route of administration of chemotherapy on unresectable, advanced gastric cancer

Zhang

Fan

et al. 2012

World J Surg Onc

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BackgroundThe aim of this study was to compare the efficacy of two neoadjuvant chemotherapies (FLEEOX and XELOX) with different routes of administration for unresectable gastric cancer.MethodsA total of 85 patients with unresectable gastric cancer hospitalized from January 2007 to December 2009 received neoadjuvant chemotherapy. The FLEEOX group (48 patients) received the FLEEOX regimen(fluorouracil, leucovorin, http://epirubicin, epotoside, and oxaliplatin), which combined arterial with venous administration for one or two cycles, while the XELOX group (37 patients) received XELOX (capecitabine plus oxaliplatin) via venous administration for two to four cycles. The clinical response and overall survival of the two groups were compared.ResultsIn the FLEEOX group, the clinical response rate (RR) of chemotherapy was 85.4% (41 of 48 patients) and the median survival time was 25 months. The 1-year and 2-year disease-free survival (DFS) rates were 85.4% and 45.8%, respectively. In the XELOX group, the clinical RR was 59.5% and the median survival time was 9 months, while the 1-year and 2-year survival rates were 35.2% and 8.3%, respectively. The clinical RR, the R0 resection rate, the median survival time, and the 1-year and 2-year DFS rates were significantly better (P < 0.05) in the FLEEOX group than in the XELOX group. In addition, there were no significant differences in the rates of toxic and adverse reactions or post-operative complications between the two groups.ConclusionsFor patients with a preoperative diagnosis of unresectable gastric cancer, the efficacy of the FLEEOX regimen, which combines arterial with venous administration, was better than that of the XELOX regimen, using venous administration only. This combination of arterial and venous administration could be useful for improving the efficacy of neoadjuvant chemotherapy for gastric cancer.

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Section: Introductionmentioning

confidence: 99%

Comparison of efficacy of different route of administration of chemotherapy on unresectable, advanced gastric cancer

Zhang

Fan

et al. 2012

World J Surg Onc

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“…The most significant advantage of the LAMP method is the ability to amplify specific sequences of DNA under isothermal conditions between 63 • C and 65 • C. As a result, it requires only simple and inexpensive equipment available in typical hospital laboratories. We developed the LAMP method for detection of six human herpesviruses Yoshikawa et al, 2004;Okamoto et al, 2004;Enomoto et al, 2005;Kimura et al, 2005;Sugiyama et al, 2005;Suzuki et al, 2006). Furthermore, we found that herpes simplex virus DNA could be detected directly in swab samples by the LAMP method without DNA extraction .…”

Section: Introductionmentioning

confidence: 99%

Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Ihira

Akimoto

Miyake

et al. 2007

Journal of Clinical Virology

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“…The technique, termed loop-mediated isothermal amplification (LAMP), requires a set of conditions and primers different from those used for PCRs (8,12,14,15). LAMP assays have now been reported for several pathogens (8,9,12,14,15,18,20). The reaction typically occurs over 30 to 60 min under isothermal conditions with temperatures ranging from 60 to 65°C and can be conducted with a simple heating block.…”

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confidence: 99%

Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus

et al. 2007

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Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advantages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can potentially be developed for "point of care" screening of BKV.

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Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA

Cited by 34 publications

References 25 publications

Comparison of efficacy of different route of administration of chemotherapy on unresectable, advanced gastric cancer

Comparison of efficacy of different route of administration of chemotherapy on unresectable, advanced gastric cancer

Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method

Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus

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