Development of the reversible PGA immobilization by using the immobilized metal ion affinity membrane

Chen, Chih-I; Ko, Yi-Miao; Lien, Wei-Ling; Lin, Yi‐Hsuan; Li, I-Tsang; Chen, Chien‐Hsun; Shieh, Chwen‐Jen; Liu, Yung-Chuan

doi:10.1016/j.memsci.2012.01.022

Cited by 11 publications

(2 citation statements)

References 28 publications

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“…Miranda has done research on immobilized PGA regeneration: first, the study on the regeneration of immobilized PGA found that even highly stable enzyme preparations may be inactivated for a long time in the presence of organic co‐solvents or high temperatures, thus reducing the operational life of the biocatalyst. Besides, Miranda et al further carried out two strategies for the regeneration of immobilized PGA, one was to expand it in an aqueous medium, which increased the enzyme activity from 36.0% to 62.8%. The other way is by adding 30% ethylene glycol (EG) in the aqueous medium, which increased the enzyme activity from 50.0% to 68.4%.…”

Section: Immobilization Pgamentioning

confidence: 99%

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

Mohammed

Zhou

et al. 2019

Polymers for Advanced Techs

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Immobilized penicillin G acylase (PGA) as an important industrial catalyst can catalyze penicillin G potassium (PG) to 6‐aminopenicillanic acid (6‐APA). 6‐APA is an important intermediate for semisynthetic penicillin drugs, which occupies a huge market space in the anti‐inflammatory field; as a result, immobilized PGA occupies a huge market space in the pharmaceutical field. However, at present, there are different degrees of defects in the preparation and production process of immobilized PGAs on the market because of the huge demand; therefore, the performance of immobilized PGA and its productivity will bring huge economic benefits to enterprises. Therefore, research on immobilized PGA has always been a focus. This review first introduces the source, classification, structure, and catalytic mechanism of PGA and then studies the development of immobilization methods, immobilized carriers, reaction media, enzyme activity regeneration, and reactors of immobilized PGA in recent years.

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Section: Immobilization Pgamentioning

confidence: 99%

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

Mohammed

Zhou

et al. 2019

Polymers for Advanced Techs

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“…Immobilized metal ion affinity membrane (IMAM) has been used increasingly as an effective means for bio-separations. [1][2][3][4][5] A major challenge in this technique is the preparation of the available IMAM from suitable polymeric membrane substrate. 6 The desired membrane substrate should have high hydrophilicity, microporous structure, narrow pore size distribution, high density of functional groups (such as -OH, -COOH, -SH, -NH 2 , -N + R 3 , -SO 3 À , etc.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of copper ions on chitosan/cellulose acetate blend hollow fiber membrane for protein adsorption

Shen

Yang

Liu³

et al. 2017

RSC Adv.

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Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His‐tagged protein

Zeng

Jin

Yang

et al. 2018

J of Applied Polymer Sci

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Based on a crosslinked chitosan (CS)/polyvinyl alcohol (PVA) matrix membrane, an immobilized metal ion affinity membrane (IMAM) using Cu 2+ and Ni 2+ ions as affinity ligands was prepared for purification of the His-tagged recombinant protein. The affinity membrane possessed a favorable membrane structure including 1.39 μm average pore size and 0.33 mLÁcm −2 Ás −1 water flux under 0.08 MPa pressure at 25 C. The Cu 2+ and Ni 2+ ions capacities immobilized on the IMAM were 155.6 and 137.3 μmolÁdisk −1 , respectively. The IMAM had an excellent specific affinity to His-tagged protein. About 10-fold purification factor for the model protein was obtained in a batch adsorption, and serine hydroxymethyl transferase could be purified to a single band in sodium dodecyl sulfatepolyacrylamide gel electrophoresis analysis from its crude extract solution with an affinity membrane cartridge by a dynamic purification process. This work provides a promising IMAM for the purification of His-tagged recombinant proteins.

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Development of the reversible PGA immobilization by using the immobilized metal ion affinity membrane

Cited by 11 publications

References 28 publications

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

Immobilization of copper ions on chitosan/cellulose acetate blend hollow fiber membrane for protein adsorption

Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His‐tagged protein

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