Development of the seminiferous tubules after neonatal hemicastration in the boar

Kosco, M. S.; Loseth, K. J.; Crabo, B. G.

doi:10.1530/jrf.0.0870001

Cited by 28 publications

(31 citation statements)

References 20 publications

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“…Whilst total Sertoli cell numbers at 25 dpp are the reciprocal of mature Sertoli cell numbers, early termination of mitosis in MS boars would result in a smaller adult Sertoli cell population and smaller testes compared with WC boars. These observations are consistent with the appearance of lumina by 28 dpp and early onset of spermatogenesis (56 dpp) in MS boars (Lunstra et al 1997), contrasting initiation of lumen formation by 94 dpp (Kosco et al 1989), and completion by 120 dpp (Tran et al 1981) in WC boars. These observations further illustrate the considerable breed differences in timing of Sertoli cell maturation and blood-testis barrier formation, and thus duration of the Sertoli cell proliferation period.…”

Section: Discussionsupporting

confidence: 76%

Stereological evaluation of Sertoli cell ontogeny during fetal and neonatal life in two diverse breeds of swine

McCoard¹,

Wise²,

Lunstra³

et al. 2003

Journal of Endocrinology

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Chinese Meishan (MS) boars have smaller testes due to fewer Sertoli cells compared with White Composite (WC) boars. The objective was to describe Sertoli cell development relative to circulating FSH concentrations in fetal and neonatal MS and WC boars. Testes and blood samples were collected on days 60, 75, 90 and 105 postcoitum (dpc) and 1, 7, 14 and 25 postpartum (dpp). One testis was immunostained for GATA4 or Ki67 antigen to evaluate total and proliferating Sertoli cell numbers respectively. Testicular size was greater (P,0·01) in WC than MS boars at all ages, associated with a greater mass of interstitial tissue. Tubular mass (P,0·01) was greater in prenatal WC boars, but postnatally increased more rapidly (P,0·001) in MS boars, exceeding WC boars by 25 dpp. Sertoli cell numbers increased with age, was greater (P,0·001) in WC than MS boars during prenatal development but increased rapidly (P,0·01) by 1 dpp in MS and thereafter was similar in both breeds. The proportion of Ki67-positive Sertoli cells was maximal at 90 dpc, declining thereafter, did not differ between breeds through 7 dpp, but was greater (P,0·05) in WC than MS boars at 14 and 25 dpp. Plasma FSH concentrations were greater (P,0·05) in WC than MS boars at 75 dpc. FSH concentrations were elevated at 105 dpc (MS) and 1 dpp (WC) but declined thereafter with advancing postnatal age in both breeds. This study illustrates that late gestation represents the period of maximal Sertoli cell proliferation. Despite asynchronous Sertoli cell population growth between breeds during early postnatal life, differential mature Sertoli cell numbers and testicular size are probably due to differences in duration of the proliferative period after 25 dpp, potentially regulated by Sertoli cell maturation and blood-testis barrier formation. These events were not associated with fetal or early postnatal changes in FSH secretion.

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Section: Discussionsupporting

confidence: 76%

Stereological evaluation of Sertoli cell ontogeny during fetal and neonatal life in two diverse breeds of swine

McCoard¹,

Wise²,

Lunstra³

et al. 2003

Journal of Endocrinology

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“…The porcine spermatogenesis during the postnatal development is not well understood because of the limited number of studies (Kosco et al 1989, Franca et al 2000, Goddard et al 2001. c-kit is known to be a protooncogene encoding a tryrosine kinase receptor in the PDGF/CSF-1 receptor family (Yarden et al 1987, Qiu et al 1988.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the signal of porcine c-kit mRNA was utilized as a control in the present study, and was observed in the testes at all stages from day 13 to adult stage. Porcine male germ cells have been reported to proliferate continuously in seminiferous tubules during postnatal stages (Kosco et al 1989, Franca et al 2000. The diameter of seminiferous tubules and number of spermatogenic cells have been shown to increase gradually from day 94 to 122, and the onset of spermiogenesis has been observed at day 94 (Kosco et al 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Porcine male germ cells have been reported to proliferate continuously in seminiferous tubules during postnatal stages (Kosco et al 1989, Franca et al 2000. The diameter of seminiferous tubules and number of spermatogenic cells have been shown to increase gradually from day 94 to 122, and the onset of spermiogenesis has been observed at day 94 (Kosco et al 1989). In the present study, histological examination of porcine testes throughout the period of postnatal development indicated that the diameter of seminiferous tubules and number of germ cells increased from day 96 to 110, and spermatozoa were observed in the testes at day 110.…”

Section: Discussionmentioning

confidence: 99%

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Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase Cζ

Yoneda

Kashima²,

Yoshida³

et al. 2006

Reproduction

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The molecular mechanism by which sperm triggers Ca 2C oscillation, oocyte activation, and early embryonic development has not been clarified. Recently, oocyte activation has been shown to be induced by sperm-specific phospholipase Cz (PLCz). The ability of PLCz to induce oocyte activation is highly conserved across vertebrates. In the present study, porcine PLCz cDNA was identified and the nucleotide sequence was determined. The expression pattern of porcine PLCz mRNA during the period of postnatal testicular development was shown to be similar to that of mouse PLCz. PLCz mRNA expression in the pig and mouse was detected only in the testes when the elongated spermatids had differentiated, and was detected from day 96 after birth in the pig. Histological examination of porcine testis during the period of postnatal development revealed the presence of spermatozoa from day 110 after birth. These findings suggest that the synthesis of PLCz mRNA starts when spermiogenesis is initiated. Microinjection of porcine PLCz complementary RNA into porcine oocytes demonstrated that porcine PLCz has the ability to trigger repetitive Ca 2C transients in porcine oocytes similar to that observed during fertilization. It was also found that porcine PLCz cRNA has the potential to induce oocyte activation and initiate embryonic development up to the blastocyst stage.

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“…The sensitivity of these steps to FSH has also been shown in mature rats treated with a GnRH antagonist [37] or immunized against GnRH [28] as well as in juvenile and adult monkeys [5,27,41] and hpg mice [36]. Finally, hemicastration of boars at a young age has also been shown to increase Sertoli cell numbers in the remaining testis [26,33] accompanied by a transient increase in blood FSH levels [25].…”

mentioning

confidence: 99%

Ram lambs need FSH for normal testicular growth, Sertoli cell numbers and onset of spermatogenesis

Kilgour

Pisselet²,

Dubois³

et al. 1998

Reprod. Nutr. Dev.

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Development of the seminiferous tubules after neonatal hemicastration in the boar

Abstract: Summary. Development of the prepubertal seminiferous tubules of the right testis was characterized morphometrically every 14 days from 10 to 122 days of age in intact boars (I)

Cited by 28 publications

References 20 publications

Stereological evaluation of Sertoli cell ontogeny during fetal and neonatal life in two diverse breeds of swine

Stereological evaluation of Sertoli cell ontogeny during fetal and neonatal life in two diverse breeds of swine

Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase Cζ

Ram lambs need FSH for normal testicular growth, Sertoli cell numbers and onset of spermatogenesis

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