Development of the short time exposure (STE) test: An in vitro eye irritation test using SIRC cells

Takahashi, Yutaka; Koike, Mirei; Honda, Hiroshi; Itô, Yûichi; Sakaguchi, Hitoshi; Suzuki, Hiroyuki; Nishiyama, Naohiro

doi:10.1016/j.tiv.2007.11.018

Cited by 86 publications

(66 citation statements)

References 16 publications

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“…Moreover, the STE test was able to detect the irritation potential over a range irritation potentials (minimally, moderate, and severe irritant). Furthermore, poorly water-soluble chemicals such as toluene, octanol, and hexanol could be evaluated in the STE test by using mineral oil as the vehicle (Takahashi et al, 2008). For these reasons, the STE test could be considered a building block assay in the tiered approach to establishing an ocular irritation animal alternative testing paradigm.…”

Section: Discussionmentioning

confidence: 99%

“…However, no in vitro eye irritation test has been approved by OECD so far. Accordingly, we developed the short time exposure (STE) test based on cytotoxicity test that used rabbit corneal cell line (SIRC) cells (Takahashi, 2007;Takahashi et al, 2008). The STE test was well characterized in terms of employing cultured cells lines derived from cornea and possessing similar or shorter exposure times than many other cytotoxicity based methods, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the STE test has the advantage of being able to evaluate the eye irritation potential of water insoluble chemicals (e.g. toluene and hexanol) and the eye irritation ranking of the STE test based on the prediction model (PM) were well correlated with the Draize test (Takahashi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Inter-laboratory study of short time exposure (STE) test for predicting eye irritation potential of chemicals and correspondence to globally harmonized system (GHS) classification

et al. 2009

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-Short time exposure (STE) test using rabbit corneal cell line (SIRC) cells was developed as an alternative eye irritation test. STE test uses relative viability as the endpoint after cells are exposed to the test material at constant concentrations for 5 min. In this inter-laboratory study with 3 laboratories, 44 chemicals with a wide range of classes were evaluated for the transferability, between-lab reproducibility and predictive capacity of the STE test as an alternative eye irritation test. Globally harmonized sysin vivo data. Transferability was assessed using standard chemicals (sodium lauryl sulfate, calcium thioglycolate, andThe irritation category (Irritant or Non irritant) at each test concentration (5% and 0.05%) in STE test was --el (PM) based on the relative viability at two concentrations (5% and 0.05%) were highly correlated with the GHS ranks of non-irritant, category 1, and category 2, respectively (accuracy was 75.0% at all laboratories). These results suggest that the STE test possessed easy transferability, reproducibility, good predictive performance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inter-laboratory study of short time exposure (STE) test for predicting eye irritation potential of chemicals and correspondence to globally harmonized system (GHS) classification

et al. 2009

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“…Resazurin at 0.01% was added to each well and evaluated after 4 hours using a fluorescence spectrophotometer (SpectraFluorPlus, Tecan, Maennedorf, Switzerland) with a stirrer at an excitation wavelength of 530 nm and an emission wavelength of 590 nm. 25,26 The experiment was done in triplicate.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Chemical and Antidiarrheal Studies ofPlinia cauliflora

Souza-Moreira

Severi

Santos

et al. 2011

Journal of Medicinal Food

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Plinia cauliflora (Mart.) Kausel, widespread in South America, has edible fruits, and its bark is commonly used against diarrhea and other disorders, on account of its astringency. Because diarrhea is still one of the most important causes of illness and death among children in developing countries, where the population turns to traditional medicine for its treatment, the present study determined the composition of fruit and leaf extracts of P. cauliflora, analyzed the activity against diarrhea by antimicrobial and gastrointestinal motility, and evaluated the cytotoxicity of the extracts. Chemical composition was determined by high-performance liquid chromatograpy-ultraviolet/photodiode array detection. Antimicrobial activity was analyzed by agar diffusion and the microdilution method against etiological agents of diarrhea. The effect on gastrointestinal motility was analyzed using an experimental model in mice. Cytotoxicity was evaluated in vitro with the fibroblast cell line SIRC CCL 60, and leaf extract showed a 50% inhibitory concentration of 0.48 lg/mL. Gallic acid, ellagic acid, and flavonoid derivatives were detected in the extracts. It was observed that fruit and leaf extracts showed some activity against Enterococcus faecalis, Escherichia coli, Salmonella sp., and Shigella sp. However, neither extract had any effect on gastrointestinal motility.

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“…La classification dans la catégorie 1 est fondée sur le potentiel corrosif pour la peau d'une solution d'hydroxyde de sodium à 100 % (sur la liste des produits chimiques d'épreuve potentiellement corrosifs pour la peau dans la LD 435 de l'OCDE) et sur les critères de classification en catégorie 1 du SGH de l'ONU (1). 4 La classification dans les catégories 2A ou 2B dépend de l'interprétation des critères du SGH de l'ONU pour distinguer ces deux catégories, à savoir que l'on doit constater des effets chez 2 animaux sur 6 ou 4 animaux sur 6 le septième jour pour classer le produit dans la catégorie 2A. Les données in vivo sont issues de 2 études menées chacune sur 3 animaux.…”

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Essai n° 491 : Méthode d'essai d'exposition de courte durée in vitro pour l'identification des produits chimiques i) provoquant des lésions oculaires graves ou ii) ne relevant d'aucune classification pour irritation oculaire ou lésion oculaire grave

2023

Lignes Directrices De l'OCDE Pour Les Essais De Produits Chimiques, Section 4

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Development of the short time exposure (STE) test: An in vitro eye irritation test using SIRC cells

Cited by 86 publications

References 16 publications

Inter-laboratory study of short time exposure (STE) test for predicting eye irritation potential of chemicals and correspondence to globally harmonized system (GHS) classification

Inter-laboratory study of short time exposure (STE) test for predicting eye irritation potential of chemicals and correspondence to globally harmonized system (GHS) classification

Chemical and Antidiarrheal Studies ofPlinia cauliflora

Essai n° 491 : Méthode d'essai d'exposition de courte durée in vitro pour l'identification des produits chimiques i) provoquant des lésions oculaires graves ou ii) ne relevant d'aucune classification pour irritation oculaire ou lésion oculaire grave

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