Development of the simultaneous analysis of choline salicylate, lidocaine hydrochloride and preservatives in a new dental gel by HPLC method

Abstract

A new high-performance liquid chromatography (HPLC) method for the simultaneous quantitative determination of active pharmaceutical substances and preservatives in a new dental medication has been developed. The optimization of HPLC method parameters was done through studies of a mobile phase composition and a detection wavelength. Our developing method uses an ACE C18 column (250 × 4.6 mm, 5 µm) and a gradient mode for separation with the acetonitrile and phosphate buffer solution (adjusted to pH 3.0) as mobile phases. The flow rate is 1 ml/ min, and the detection was set at 260 nm (DAD). The method was evaluated according to the ICH guidelines and the State Pharmacopoeia of Ukraine in terms of specificity, accuracy, linearity and precision (repeatability and intermediate precision). The limit of detection and the limit of quantification were also calculated. The developed method was put in place for the analysis of a combined dental gel to a quantitative determination of the APIs (choline salicylate, lidocaine hydrochloride) and preservatives (methylparaben, propylparaben).

An isocratic RP-HPLC-UV method for simultaneous quantification of tizanidine and lidocaine: application to in vitro release studies of a subcutaneous implant

“…Previous literature reports have described the quantication of both drugs for various purposes. Methods for quantifying LD were developed in conjunction with preservatives 18 and other drugs, serving as antiseptics, 19 antiparasitic, 20 antifungal, 20 anti-inammatory, 21 analgesic, 22 mucolytic, 23 antiviral 24,25 and corticosteroids. 21 Additionally, other validated methods exist for determining the drug in polymeric matrices, [26][27][28] plasma, 29,30 and in new topical dosage forms.…”

RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel

“…This drug is used to reduce pain during some medical operations. [5][6][7] Raising the threshold for electrical excitation in nerves causes numbness in the mouth, throat, or nose before these procedures. [5][6][7] By decreasing the spread of nerve impulses and lowering the pace at which action potentials rise, it successfully prevents the creation and conduction of nerve impulses.…”