Development of the vomeronasal amygdala in anuran amphibians: Hodological, neurochemical, and gene expression characterization

Moreno, Nerea; González, Agustı́n

doi:10.1002/cne.21422

Cited by 36 publications

(46 citation statements)

References 84 publications

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“…Rabbit anti-GABA shows positive binding with GABA in a dot blot assay and negative binding with bovine serum albumin (BSA; Sigma data sheet). The GABA antibody has been extensively used in amphibians (Bachy and Retaux, 2006;Moreno and Gonzalez, 2007) and was also used in a recent study in adult Drosophila to describe the morphology of GABAimmunoreactive local interneurons in the AL (Seki et al, 2010), thereby demonstrating specificity for GABAergic interneurons in the antennal lobe of Drosophila, similar to our analysis on the same type of neuron in the same species at the larval stage. Even Larkin et al (2010), also using rabbit anti-GABA, showed a similar pattern of GABA-positive neurons within the larval antennal lobe in comparison with our study.…”

Section: Anti-gabasupporting

confidence: 70%

Diversity, variability, and suboesophageal connectivity of antennal lobe neurons in D. melanogaster larvae

Thum

Leisibach

Gendre

et al. 2011

J of Comparative Neurology

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Whereas the ''vertical'' elements of the insect olfactory pathway, the olfactory receptor neurons and the projection neurons, have been studied in great detail, local interneurons providing ''horizontal'' connections in the antennal lobe were ignored for a long time. Recent studies in adult Drosophila demonstrate diverse roles for these neurons in the integration of odor information, consistent with the identification of a large variety of anatomical and neurochemical subtypes. Here we focus on the larval olfactory circuit of Drosophila, which is much reduced in terms of cell numbers. We show that the horizontal connectivity in the larval antennal lobe differs largely from its adult counterpart. Only one of the five anatomical types of neurons we describe is restricted to the antennal lobe and therefore fits the definition of a local interneuron. Interestingly, the four remaining subtypes innervate both the antennal lobe and the suboesophageal ganglion. In the latter, they may overlap with primary gustatory terminals and with arborizations of hugin cells, which are involved in feeding control. This circuitry suggests special links between smell and taste, which may reflect the chemosensory constraints of a crawling and burrowing lifestyle. We also demonstrate that many of the neurons we describe exhibit highly variable trajectories and arborizations, especially in the suboesophageal ganglion. Together with reports from adult Drosophila, these data suggest that wiring variability may be another principle of insect brain organization, in parallel with stereotypy.

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Section: Anti-gabasupporting

confidence: 70%

Diversity, variability, and suboesophageal connectivity of antennal lobe neurons in D. melanogaster larvae

Thum

Leisibach

Gendre

et al. 2011

J of Comparative Neurology

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“…All amphibians possess a vomeronasal organ and an accessory olfactory bulb [Reiss and Eisthen, 2008], but the projections of the accessory olfactory bulb have been determined experimentally only in anurans [Northcutt and Royce, 1975;Scalia et al, 1991;Moreno and González, 2007] and salamanders [Laberge and Roth, 2005]. It is now confirmed that lepidosirenid lungfishes also possess an accessory olfactory bulb whose primary, if not sole, target is the ipsilateral medial amygdalar nucleus [González et al, 2010], as is the case for anurans and salamanders ( fig.…”

Section: Phylogenetic Considerationsmentioning

confidence: 52%

“…In the white sturgeon, BDA and biocytin are more effective than DiI in labeling secondary olfactory projections, due to the large number of myelinated fibers in the lateral olfactory tract [Northcutt, 2011b], whereas BDA and DiI appear to be equally effective in labeling the pallial connections in goldfish [Northcutt, 2006]. Although biocytin is generally considered a more effective tracer than HRP, both tracers, as well as BDA, reveal comparable secondary olfactory projections in amphibians [Scalia et al, 1991;Laberge and Roth, 2005;Moreno and González, 2007]. The possible bias resulting from the use of different tracers notwithstanding, the available data indicate that there has been a major reduction in the number of telencephalic areas that receive secondary olfactory projections, particularly in anurans.…”

Section: Phylogenetic Considerationsmentioning

confidence: 99%

Olfactory Projections in the Lepidosirenid Lungfishes

Northcutt

Rink²

2011

Brain Behav Evol

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Olfactory nerve and olfactory bulb projections in lepidosirenid lungfishes were experimentally determined with neural tracers. Unilateral injections of DiI into the olfactory nerve labeled the accessory and main olfactory bulbs as well as fibers of the anterior root of the terminal nerve, which terminates extensively in cell groups of the medial hemispheric wall, the dorsal and lateral pallia, and the preoptic nuclei and posterior tubercle. Lepidosirenid lungfishes do not exhibit separate vomeronasal nerves, but previous data indicate that calbindin-positive receptors within basal crypts of the olfactory epithelium are homologous to the vomeronasal organ of tetrapods. Unilateral injections of DiI into the accessory olfactory bulb reveal an accessory olfactory tract which terminates primarily if not solely in the ipsilateral medial amygdalar nucleus as in amphibians. Unilateral injections of tracers into the main olfactory bulb reveal extensive projections to all cell groups in the ipsilateral telencephalic hemisphere, except for the medial amygdalar nucleus, as well as secondary olfactory projections (decussating in the habenular commissure) to the contralateral dorsal pallium and main olfactory bulb. Secondary olfactory projections also terminate bilaterally in diencephalic and midbrain centers after partial decussation in the anterior and postoptic commissures, as well as in the ventral hypothalamus and posterior tubercle. Cladistic analysis of the extensive secondary olfactory projections indicates that this pattern is primitive for all bony fishes whereas the reduction in secondary olfactory projections in amphibians, particularly anurans, is a derived, simplified pattern.

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“…The pattern of staining obtained with this antibody was similar to that obtained with in situ hybridization for the xGAD67 gene, which encodes an isoform of glutamic acid decarboxylase, an enzyme involved in the synthesis of GABA (compare the results of Brox et al, 2003, andGonzález, 2007a).…”

Section: Controls and Specificity Of The Antibodiesmentioning

confidence: 54%

Characterization of the bed nucleus of the stria terminalis in the forebrain of anuran amphibians

Moreno

Morona

López

et al. 2011

J of Comparative Neurology

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Major common features have been reported for the organization of the basal telencephalon in amniotes, and most characteristics were thought to be acquired in the transition from anamniotes to amniotes. However, gene expression, neurochemical, and hodological data obtained for the basal ganglia and septal and amygdaloid complexes in amphibians (anamniotic tetrapods) have strengthened the idea of a conserved organization in tetrapods. A poorly characterized region in the forebrain of amniotes has been the bed nucleus of the stria terminalis (BST), but numerous recent investigations have characterized it as a member of the extended amygdala. Our study analyzes the main features of the BST in anuran amphibians to establish putative homologies with amniotes. Gene expression patterns during development identified the anuran BST as a subpallial, nonstriatal territory. The BST shows Nkx2.1 and Lhx7 expression and contains an Islet1-positive cell subpopulation derived from the lateral ganglionic eminence. Immunohistochemistry for diverse peptides and neurotransmitters revealed that the distinct chemoarchitecture of the BST is strongly conserved among tetrapods. In vitro tracing techniques with dextran amines revealed important connections between the BST and the central and medial amygdala, septal territories, medial pallium, preoptic area, lateral hypothalamus, thalamus, and prethalamus. The BST receives dopaminergic projections from the ventral tegmental area and is connected with the laterodorsal tegmental nucleus and the rostral raphe in the brainstem. All these data suggest that the anuran BST shares many features with its counterpart in amniotes and belongs to a basal continuum, likely controlling similar reflexes, reponses, and behaviors in tetrapods.

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Development of the vomeronasal amygdala in anuran amphibians: Hodological, neurochemical, and gene expression characterization

Cited by 36 publications

References 84 publications

Diversity, variability, and suboesophageal connectivity of antennal lobe neurons in D. melanogaster larvae

Diversity, variability, and suboesophageal connectivity of antennal lobe neurons in D. melanogaster larvae

Olfactory Projections in the Lepidosirenid Lungfishes

Characterization of the bed nucleus of the stria terminalis in the forebrain of anuran amphibians

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