Development of thermo-photo sensitive genic male sterile lines in wheat using doubled haploid breeding

Abstract

Background: Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past 20 years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids. Application of doubled haploid (DH) breeding provides a useful strategy to efficiently develop practically usable TPSGMS lines. Results: F 1 s and selected F 2 and F 3 sterile plants of eight crosses made from two commercial TPSGMS lines were used to produce DH lines. We developed a total of 24 elite DH sterile lines with stable sterility, good outcrossing and yield potential, resistance to yellow rust and powdery mildew, as well as desirable plant height (50-60 cm). These DH lines were developed within 4 years through at least 1 year of evaluation. The stability of male sterility was confirmed for most (20/24) of these elite DH sterile lines by multiple tests in two or 3 years. These lines are expected to be used in hybrid wheat breeding. The percentage of elite lines developed from the tested DH lines produced from filial generations was in the order of F 2 > F 3 > F 1. Conclusions: We demonstrate that coupling DH techniques with conventional breeding is an efficient strategy for accelerating the development of more practical wheat TPSGMS lines. Generation of DHs from F 2 generation appeared to be the better choice considering the balance of shortening breeding time and overall breeding efficiency.

Technology for Production of Wheat Doubled Haploid via Maize Pollen Induction—Updated Review

“…Thus, the Wheat × Maize System became popular and was often used to produce haploid plants from commercial wheat varieties [31][32][33]. Now, the Wheat × Maize System acts as one of the most practical methods for wheat [34][35][36].…”

“…Over the years, the Wheat × Maize System has been gradually upgraded and now offers a powerful tool for research on wheat genetics and breeding. This includes applications such as molecular cytogenetic characterization [68], the development of thermophoto sensitive genic male sterile lines [36], QTL mapping [69], and so on.…”

Advances in Gene Editing of Haploid Tissues in Crops

“…For example, the generation of hybrid crops requires an efficient male sterility system (MS). Wheat hybrid breeding programs in China increasingly rely on thermo-photo-sensitive genic male sterility (TPSGMS) [7]. The TPSGMS lines are sterile under low-temperature and short-day conditions that allow for hybrid grain production.…”

“…It was possible to generate only two TPSGMS lines in 14 years of conventional breeding. At the same time, the authors developed 24 elite TPSGMS lines with complete homozygosity in four years using DH techniques [7]. In addition to breeding, the HI technique benefits studying plant genetics and basic embryology [6,8].…”

Detection of seed purity of hybrid wheat using reflectance and transmittance hyperspectral imaging technology

“…The application of wheat heterosis can improve yield potential and stability, which is considered an important approach to overcome food shortage ( Boeven et al., 2018 ). Currently, the chemical hybridizing agent (CHA) and genic male sterility (GMS) systems are widely used as a two-line hybrid system in hybrid wheat production because they do not require maintenance or any pre-propagation ( Zhao, 2010 ; Murai et al., 2016 ; Li et al., 2020 ). However, CHA and GMS are highly dependent on the crop growth stage and weather, and the female parent is often not completely sterile ( Singh et al., 2010 ).…”