Development of thermostable amylase enzyme from Bacillus cereus for potential antibiofilm activity

Ramalingam, Vaikundamoorthy; Rajendran, R.; Selvaraju, Ananth; Moorthy, Kaviyarasan; Santhanam, P.

doi:10.1016/j.bioorg.2018.02.014

Cited by 66 publications

(21 citation statements)

References 48 publications

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“…The purified enzyme caused 68.33%, 64.84%, 61.81%, and 59.2% of inhibition in V. cholerae (VC5, VC26), MRSA (102), and P. aeruginosa ATCC10145, respectively. Another study by Vaikundamoorthya et al (2018) confirmed the antibiofilm efficacy of the thermostable amylase enzyme from B. cereus. It is worth noting that the isolated bacteria filtrate showed great antibiofilm activity compared to the purified amylase enzyme from the selected isolates.…”

Section: Antibiofilm Activity Of Isolated Bacterial Filtrate and Purimentioning

confidence: 85%

“…This family also includes maltotriotic glucose, dextrin, and maltose. Amylase has exhibited excellent antibiotic activity against Pseudomonas aeruginosa and Staphylococcus aureus marine-derived biofilm-forming bacteria (Vaikundamoorthya et al, 2018). Soil is the main part of the terrestrial environment, which is compared with aquatic environments with a large association of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria

Elamary

Salem

2020

PeerJ

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Background Bacterial biofilms have become a major threat to human health. The objective of this study was to isolate amylase-producing bacteria from soil to determine the overall inhibition of certain pathogenic bacterial biofilms. Methods We used serial dilution and the streaking method to obtain a total of 75 positive amylase isolates. The starch-agar plate method was used to screen the amylolytic activities of these isolates, and we used morphological and biochemical methods to characterize the isolates. Optimal conditions for amylase production and purification using Sephadex G-200 and SDS-PAGE were monitored. We screened these isolates’ antagonistic activities and the purified amylase against pathogenic and multi-drug-resistant human bacteria using the agar disk diffusion method. Some standard antibiotics were controlled according to their degree of sensitivity. Finally, we used spectrophotometric methods to screen the antibiofilm 24 and 48 h after application of filtering and purifying enzymes in order to determine its efficacy at human pathogenic bacteria. Results The isolated Bacillus species were Bacillus megaterium (26.7%), Bacillus subtilis (16%), Bacillus cereus (13.3%), Bacillus thuringiesis (10.7%), Bacillus lentus (10.7%), Bacillus mycoides (5.3%), Bacillus alvei (5.3%), Bacillus polymyxa (4%), Bacillus circulans (4%), and Micrococcus roseus (4%). Interestingly, all isolates showed a high antagonism to target pathogens. B. alevi had the highest recorded activity (48 mm) and B. polymyxa had the lowest recorded activity (12 mm) against Staphylococcus aureus (MRSA) and Escherichia coli, respectively. On the other hand, we detected no antibacterial activity for purified amylase. The supernatant of the isolated amylase-producing bacteria and its purified amylase showed significant inhibition for biofilm: 93.7% and 78.8%, respectively. This suggests that supernatant and purified amylase may be effective for clinical and environmental biofilm control. Discussion Our results showed that soil bacterial isolates such as Bacillus sp. supernatant and its purified amylase are good antibiofilm tools that can inhibit multidrug-resistant former strains. They could be beneficial for pharmaceutical use. While purified amylase was effective as an antibiofilm, the isolated supernatant showed better results.

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Section: Antibiofilm Activity Of Isolated Bacterial Filtrate and Purimentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria

Elamary

Salem

2020

PeerJ

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“…The crystal structure of α-amylase shows that calcium ion is involved in ionic interaction between domain A and domain B between which α-amylase active site is located and thus calcium ion form an ionic bridge between these domains promoting α-amylase stability and catalytic activity. 19 In previous studies, other Bacillus species such as B. cereus, 20 B. licheniformis, 21 B. licheniformis AT70, 18 B. licheniformis ATCC 9945a 22 and B. stearothermophilus 23 have maximum amylase production in the presence of CaCl 2 .…”

Section: One Variable At a Time Approach And Plackett-burman Designmentioning

confidence: 86%

Optimization of the thermostable alkaline and Ca-dependent α−amylase production from Bacillus paralicheniformis by statistical modeling

Bekler

Yalaz

Güven

et al. 2019

JSCS

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A novel amylolytic enzyme producing thermoalkaliphilic bacterium, the source of industrially used enzymes was isolated. Isolated strain was identified by morphological, physio-biochemical tests and the 16S rRNA gene sequence analysis. The optimal conditions of enzyme activity were determined. For higher α-amylase production, the variables such as yeast extract, starch, CaCl 2 , (NH 4) 2 SO 4 , NaCl and MgSO 4 in the α-amylase production medium, the temperature and pH were screened by Plackett-Burman design and optimised using response surface methodology (RSM). The optimal conditions were found to be 0.15 g/L for starch, 0.15 mg/L for CaCl 2 and 60 °C for temperature. By using RSM model, amylase production increase was achieved sevenfold. It is showed that this method can be utilised to optimize α-amylase production in athermophilic bacteria such as Bacillus paralicheniformis.

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“…At the same time, selenium can bind to bacterial proteins and form selenocysteine (12). Bacillus cereus, an endospore-forming, grampositive bacterium, is of great economic importance due to its ability to produce various enzymes, such as amylase (13), protease (14), and cellulase (15). As probiotics, B. cereus can produce many beneficial effects for organisms.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Bacillus cereus Fermentation Process for Selenium Enrichment as Organic Selenium Source

Chen

Cong

et al. 2020

Front. Nutr.

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Selenium is an essential trace element and micronutrient for human health. Application of organic selenium in plants and microorganisms as trace element supplement is attracting more and more attention. In this study, Bacillus cereus, an important probiotic, was used for selenium enrichment with sodium selenite as selenium source. The growth curve of B. cereus was investigated, and 150 µg/ml was selected as the concentration of selenium for B. cereus fermentation. With application of response surface methodology, the optimal fermentation conditions were obtained as follows: inoculation quantity of 7%, culture temperature of 33 • C, and shaking speed of 170 rpm, leading to the maximal selenium conversion ratio of 94.3 ± 0.2%. Field emission scanning electron microscope and energy dispersive spectrometry evidenced that inorganic selenium had been successfully transformed. This study may contribute to get a strain with high Se conversion ratio, so as to extract organic selenium in the form of selenoprotein to be used for further application.

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Development of thermostable amylase enzyme from Bacillus cereus for potential antibiofilm activity

Cited by 66 publications

References 48 publications

Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria

Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria

Optimization of the thermostable alkaline and Ca-dependent α−amylase production from Bacillus paralicheniformis by statistical modeling

Optimization of Bacillus cereus Fermentation Process for Selenium Enrichment as Organic Selenium Source

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