Abstract:Background: Evidence-Based Practice (EBP) is one of the nursing professional roles that can lead them to provide the best and more effective quality patient care. However, little studies are available across china on registered nurses' attitude, skills and utilization of knowledge source for implementation of evidence based practice. Due to this the extent to which evidence based practice being utilized and implemented in nursing practice is still in its infancy across China. Objectives: The aim of this study was to assess the attitude, skills and source of knowledge on implementation of EBP among registered nurses working at selected teaching hospitals. Method: A descriptive institutional based cross-sectional study was conducted to assess nurses' attitude, skills and utilization of knowledge source for implementation of EBP. A total of 366 RN were participated in filling self-administered questionnaire. The descriptive and inferential statistical analysis was performed by using SPSS version 20. The cut point for level of statistical significance was set at < 0.05 and all tests were 2-sided. Result: Overall, registered nurses who were participated in this study had a positive attitudes towards utilization of evidence based practice (M = 3.53, SD = 0.97). However, the level of skill in performing different EBP activities (M = 2.82 SD = 1.34) and the use of knowledge source to support daily care practice (M = 3.01, SD = 0.98) were found to be low. Similarly, participants indicated that they use more human and printed resources than electronic resources for supporting their practice (mean = 3.69, SD = 1.01; mean = 2.88, SD = 0.80; and mean = 2.57, SD = 1.05) respectively. Conclusion: It is recommended that organizational and nursing educational support were needed to improve this gap through providing training, creating awareness, and enhancing nurses' abilities for utilization and implementation of evidence based practice. Additionally, it is recommended that further research is needed to identify the influence of individual and organizational factors on implementation of evidence based nursing practice.