Development of transferred xenogeneic vole embryos in mouse uteri

Widayati, Diah Tri; Ohmori, Yasushige; Wakita, Tsuyoshi; Fukuta, Katsuhiro

doi:10.1046/j.1344-3941.2003.00115.x

Cited by 3 publications

(8 citation statements)

References 24 publications

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“…However, the uterine swellings (implantation sites) containing vole embryos were smaller than those containing mouse embryos. On day 8, internal haemorrhaging was observed in the CD‐1 uterine swellings containing vole embryos, as reported in Widayati et al. (2003), but no haemorrhaging was seen in the scid uteri with vole embryos.…”

Section: Resultssupporting

confidence: 69%

“…At day 8 of gestation, internal haemorrhaging was observed in the implantation sites. Thereafter, the survival rate of vole embryos decreased markedly, and the embryos were excluded completely after day 11 (Widayati et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

“…The periodic acid Schiff reaction‐positive GMG cells, which increased markedly in the uteri with vole embryos, were considered to be NK cells (Widayati et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Uterine horns were then dissected out and immersed in the same fixative overnight at 4 °C. Uterine swellings were regarded as having a live embryo when their diameter was larger than 2.5, 2.9 and 3.4 mm on days 6, 7 and 8 of gestation, respectively, and those showing severe haemorrhage on day 8 of gestation were taken as having a dead embryo or aborted on the basis of previous observations (Widayati et al. 2003).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we presented an experimental model for interspecific pregnancy, in which allogeneic mouse and xenogeneic vole embryos were transferred bilaterally into uterine horns of female mice (Widayati et al. 2003).…”

Section: Introductionmentioning

confidence: 99%

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Distribution patterns of immunocompetent cells in the pregnant mouse uteri carrying allogeneic mouse and xenogeneic vole embryos

2004

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Mouse and vole embryos were allogeneically and xenogeneically transferred into pseudopregnant CD-1 and immunodeficient ( scid ) female mice, and we investigated the distribution of immunocompetent cells, uterine natural killer (uNK) cells, mast cells and macrophages, in the implantation sites on days 6, 7 and 8 of gestation. The survival rate of the vole embryos decreased gradually with increased gestation, but the rate was higher in the scid uteri than in the CD-1 mice. The number of uNK cells increased markedly at the mesometrial triangle and the outer decidual area in the CD-1 uteri containing vole embryos; by contrast, scid uteri having vole embryos showed almost the same number as those having mouse embryos. Mast cells were present in large numbers at the myometrium, but rarely in the decidua in all types of pregnant uteri. Cells at the myometrium were more numerous in xenogeneic than in allogeneic transfer. Many mast cells appeared in the inner decidua where xenogeneically transferred vole embryos were dead and aborted. Macrophages were present in the outer decidua and myometria in all types of pregnant uteri, and their distribution pattern did not change even in aborted uterine sites. These results suggest:(1) the response of macrophages to dead embryos is completely inhibited, (2) uNK cells and mast cells increase near dead and aborted embryos, and (3) the increment in uNK cells responding to xenogeneic embryos is suppressed in scid mice, and the suppression may contribute partly to survival of the embryos.

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Section: Resultssupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

“…The periodic acid Schiff reaction‐positive GMG cells, which increased markedly in the uteri with vole embryos, were considered to be NK cells (Widayati et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution patterns of immunocompetent cells in the pregnant mouse uteri carrying allogeneic mouse and xenogeneic vole embryos

2004

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Vascular endothelial growth factor production by rat granulated metrial gland cells and their morphological features in normal and pathological conditions

Kaloğlu

Bulut

2007

Reprod. Fertil. Dev.

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Granulated metrial gland (GMG) cells are pregnancy-specific cells that may have many functions in successful placentation and pregnancy. In the present study, changes in the rat GMG cell structure, distribution and vascular endothelial growth factor (VEGF) expression during early pregnancy were evaluated by light microscopy. Implantation sites taken from females with spontaneous abortion were also investigated. On Day 7 of pregnancy, GMG cells were distributed through the implantation and interimplantation sites. They formed metrial glands in the mesometrial triangle on Day 9, and were observed in the decidua basalis on Day 14 of pregnancy. Avidin-biotin complex immunohistochemistry revealed that GMG cells showed moderate staining for VEGF at the beginning of pregnancy and intense staining on Days 9 and 10 of pregnancy. They were localised mostly near the newly formed blood vessels. The implantation sites from spontaneously aborting females showed numerous leucocytes in the lumen of mesometrial blood vessels. In spontaneously aborting females, GMG cells showed a distinct morphology, increased in number and volume, their granules were denser and degranulation was observed. These results suggest that rat GMG cells might be a guide for placental angiogenesis and they might share a role with leucocytes in pathological conditions.

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Cellular Mechanism Underlying Embryo-Maternal Relationship in Intraspecific and Interspecific Pregnancy

Widayati

Fukuta

2012

J Kedokt Hewan

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Mouse and vole embryos were transferred into pseudopregnant CD-1 and scid female mice. Cellular changes involved in the formation of decidua in the pregnant mouse uterus up to day 8 of pregnancy were examined by histological, electron microscopic, and histochemical techniques. On day 6 of pregnancy, the vole embryos were laid in interstitium of antimesometrial side of the uterus, as well as in intraspecific pregnancy. Compared with intraspesific pregnant mouse, blood vessels were numerous in the decidua around the vole embryos in interspecific pregnancy. Both distribution and dilation of the blood vessels were increased on day 8. A part of cells in the inner cell mass had not nuclei, suggesting damaged vole embryos on day 8. At the implantation site, the uterine decidua was invaded by extravillous trophoblast (EVT) cells whose function is to destroy the walls of the uterine spiral. Moreover, this decidua was infiltrated by a population of natural killer (NK) cells and macrophage. These cells were particularly numerous in the decidua basalis at the implantation site where they come into close contact with invading EVT cells. These results suggest that interaction between NK, macrophage, and EVT provides the controlling relationship of embryo-maternal in intraspecific and interspecific pregnancy.

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Development of transferred xenogeneic vole embryos in mouse uteri

Cited by 3 publications

References 24 publications

Distribution patterns of immunocompetent cells in the pregnant mouse uteri carrying allogeneic mouse and xenogeneic vole embryos

Distribution patterns of immunocompetent cells in the pregnant mouse uteri carrying allogeneic mouse and xenogeneic vole embryos

Vascular endothelial growth factor production by rat granulated metrial gland cells and their morphological features in normal and pathological conditions

Cellular Mechanism Underlying Embryo-Maternal Relationship in Intraspecific and Interspecific Pregnancy

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