Development of transgenic cotton (Narasimha) using triple gene Cry2Ab-Cry1F-Cry1Ac construct conferring resistance to lepidopteran pest

Katta, Sumalatha; Talakayala, Ashwini; Reddy, Malireddy K.; Addepally, Uma; Mallikarjuna, G. B.

doi:10.1007/s12038-020-0006-0

Cited by 23 publications

(15 citation statements)

References 26 publications

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“…This strategy of in planta transformation has been utilized for the successful transformation of different crop species ( Kesiraju and Sreevathsa, 2017 ); including fiber crops like cotton ( Karthik et al, 2020a ; Kesiraju et al, 2020 ) and flax ( Karthik et al, 2020b ). Moreover, development of transgenic cotton resistant to H. armigera has been a pertinent endeavour by various research groups ( Tabashnik et al, 2002 ; Wu et al, 2003 ; Kurtz et al, 2007 ; Anilkumar et al, 2008 ; Singh et al, 2016 ; Han et al, 2017 ; Bajwa et al, 2020 ; Jadhav et al, 2020 ; Katta et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…Genetic engineering for the development of insect resistant plants has been considered as a revolutionary contribution in agricultural biotechnology. Transgenesis in economically important crop plants like cotton ( Chen et al, 2017 ; Ribeiro et al, 2017 ; Siddiqui et al, 2019 ; Bajwa et al, 2020 ; Jadhav et al, 2020 ; Katta et al, 2020 ); soybean ( Marques et al, 2017 ; Moghaieb et al, 2019 ; Qin et al, 2019 ; Bacalhau et al, 2020 ); maize ( Moscardini et al, 2020 ); pigeon pea ( Ghosh et al, 2017 ; Singh et al, 2018 ; Ramkumar et al, 2020 ); chick pea ( Das et al, 2017 ); cow pea ( Bett et al, 2017 ; Addae et al, 2020 ; Kumar et al, 2021 ); sweet potato ( Zhong et al, 2019 ); jute ( Majumder et al, 2020 ); castor ( Muddanuru et al, 2019 ), etc., by the introgression of insecticidal proteins encoding genes from Bacillus thuringiensis ( Bt ) resulted in plants with improved ability to mitigate the pest load. Reduced topical applications of chemical pesticides and increased agricultural productivity have been major achievements of transgenic technology ( Fleming et al, 2018 ) in crop improvement.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently began a new era of gene pyramiding (c ry1Ac and c ry2Ab in Bollgard II, Monsanto) for insect resistance which involved the introduction of gene combinations to provide broad spectrum of efficacy toward insect herbivory ( Chen et al, 2017 ; Moghaieb et al, 2019 ; Siddiqui et al, 2019 ; Katta et al, 2020 ; Muralimohan et al, 2020 ). Pyramided genes exhibited effective toxicity and also restricted evolution of resistant pest population in engineered commercial crop plants ( Razaq et al, 2019 ; Wei et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Exploitation of Novel Bt ICPs for the Management of Helicoverpa armigera (Hübner) in Cotton (Gossypium hirsutum L.): A Transgenic Approach

et al. 2021

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Cotton is a commercial crop of global importance. The major threat challenging the productivity in cotton has been the lepidopteron insect pest Helicoverpa armigera or cotton bollworm which voraciously feeds on various plant parts. Biotechnological interventions to manage this herbivore have been a universally inevitable option. The advent of plant genetic engineering and exploitation of Bacillus thuringiensis (Bt) insecticidal crystal proteins (ICPs) marked the beginning of plant protection in cotton through transgenic technology. Despite phenomenal success and widespread acceptance, the fear of resistance development in insects has been a perennial concern. To address this issue, alternate strategies like introgression of a combination of cry protein genes and protein-engineered chimeric toxin genes came into practice. The utility of chimeric toxins produced by domain swapping, rearrangement of domains, and other strategies aid in toxins emerging with broad spectrum efficacy that facilitate the avoidance of resistance in insects toward cry toxins. The present study demonstrates the utility of two Bt ICPs, cry1AcF (produced by domain swapping) and cry2Aa (produced by codon modification) in transgenic cotton for the mitigation of H. armigera. Transgenics were developed in cotton cv. Pusa 8–6 by the exploitation of an apical meristem-targeted in planta transformation protocol. Stringent trait efficacy-based selective screening of T1 and T2 generation transgenic plants enabled the identification of plants resistant to H. armigera upon deliberate challenging. Evaluation of shortlisted events in T3 generation identified a total of nine superior transgenic events with both the genes (six with cry1AcF and three with cry2Aa). The transgenic plants depicted 80–100% larval mortality of H. armigera and 10–30% leaf damage. Molecular characterization of the shortlisted transgenics demonstrated stable integration, inheritance and expression of transgenes. The study is the first of its kind to utilise a non-tissue culture-based transformation strategy for the development of stable transgenics in cotton harbouring two novel genes, cry1AcF and cry2Aa for insect resistance. The identified transgenic events can be potential options toward the exploitation of unique cry genes for the management of the polyphagous insect pest H. armigera.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exploitation of Novel Bt ICPs for the Management of Helicoverpa armigera (Hübner) in Cotton (Gossypium hirsutum L.): A Transgenic Approach

et al. 2021

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“…These seeds were soaked in distilled water and incubated for 6 hrs under dark conditions. The embryo axis from the cotton seeds were excised and infected with Agrobacterium containing pMDC100 vector with triple Bt genes and kanamycin as selectable marker gene [13] . The transformed plants were generated by following the protocol described by Katta et al (2020) [13] .…”

Section: Methodsmentioning

confidence: 99%

“…Constitutive expression of Npt-II gene under 35S promoter would facilitate the continuous expression in all parts of the plant [1,2] . The amino glycoside chain combine with ribosomal subunit in chloroplast and mitochondria where it disrupts the protein synthesis and resulted in etiolation and death of non-transformed plants [3,13] . Among transformation methods, Agrobacterium mediated system is routine and most successful for the generation of transgenic crops.…”

Section: Introductionmentioning

confidence: 99%

An efficient and rapid kanamycin screening assay for identifying true transgenics in cotton

Katta¹,

Addepally²,

Mallikarjuna³

2021

J Pharmacogn Phytochem

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Genetic engineering is the best suitable approach for the generation of insect resistant cotton in private and public funded laboratories. Neomycin Phosphotransferase-II is the most commonly used selectable marker gene that can catabolize to kanamycin resistant protein which helps in screening and differentiation of resistant and sensitive plants. In current paper, the optimal concentration of kanamycin was determined as 200 mg/L in control plants. Based on this standardized a procedure for screening transformed plants by spraying 200 mg/L at seedling stage. The resistant plants were reconfirmed by PCR using Npt II specific primers. About 51% and 45% of kanamycin resistant plants were showed amplification of Npt II gene at T1 and T2 generation respectively. This method is simple and fast which can be exploited for screening large number of seedlings with low cost in effective manner.

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Transgenics and Crop Improvement

Koul

2022

Cisgenics and Transgenics

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Development of transgenic cotton (Narasimha) using triple gene Cry2Ab-Cry1F-Cry1Ac construct conferring resistance to lepidopteran pest

Cited by 23 publications

References 26 publications

Exploitation of Novel Bt ICPs for the Management of Helicoverpa armigera (Hübner) in Cotton (Gossypium hirsutum L.): A Transgenic Approach

Exploitation of Novel Bt ICPs for the Management of Helicoverpa armigera (Hübner) in Cotton (Gossypium hirsutum L.): A Transgenic Approach

An efficient and rapid kanamycin screening assay for identifying true transgenics in cotton

Transgenics and Crop Improvement

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