Development of Transplanted Imaginal Disks in X-irradiated Housefly Pupae
Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
Cited by 1 publication
References 7 publications
A new technique for transplanting imaginal disks of the housefly is described. When prepupal disks are transplanted superficially (surface transplantation) just beneath the puparium in early prepupal hosts, they evaginate synchronously with the host's organs and differentiate normally like the disks developing in situ. Histological evidence indicates that cells from the peripheral region of the disks migrate and join the epidermis of the host. This facilitates continuation of morphogenetic events culminating in eversion of the transplant. The significance of the cellular contact with epidermal cells of the host is discussed.A well developed nerve from the transplanted leg joins the thoraco-abdominal ganglion of the host. Flexion of the terminal leg segments indicates the presence of motor fibers innervating muscles in the leg.Disks from late third instar larvae and young prepupae evaginate whereas disks from younger third instar larvae fail to evaginate when surface transplanted. Disks, from all types of donors, cultured in adult abdomens fail to evaginate when retransplanted in prepupae. These and other results suggest that hormones may induce or inhibit morphogenetic cell movement in imaginal disks.The classical method of transplanting imaginal disks in Diptera, described by Ephrussi and Beadle ('36), has since been used extensively in investigations of various aspects of growth, determination and differentiation of these imaginal organ rudiments (see the reviews by Bodenstein, '53; Hadorn, '66; Ursprung, '66). In this method the disks are deposited within the body cavity of the host using a microinjection assembly. Disks thus transplanted in larval hosts metamorphose simultaneously with the hosts, but do not evaginate. The various segments remain telescoped within the peripodial sac and are difficult to identify except by microscopical examination of the cuticular pattern.Recently, we discovered a simple method of transplanting the disks in the prepupal stage which results in evagination of the organs as in normal development. The everted organ is in cellular contact with the epidermis and also develops nerve connection to the ganglion of the host. The significance of the epidermal contact is illustrated by the inhibition of disk development caused by irradiated epidermal cells (Bhaskaran and Sivasubramanian, '69). This system is therefore of great value in investigations of certain aspects of developmental biology and neurophysiology of insects. The present paper deals with a description of the technique and the structure of evaginated transplants. We have also presented some experimental evidence indicating the possible role of ecdysone in triggering morphogenetic cell movements in imaginal disks. MATERIAL AND METHODSThe Indian housefly Musca domesticu nebulo (Fabr) was used. Imaginal disks were dissected out in sterile ringer's solution (Ephrussi and Beadle, '36). With a razor blade the donor larva was cut just behind the ganglionic mass and a second time behind the mouth hook. All the leg disks ar...
A new technique for transplanting imaginal disks of the housefly is described. When prepupal disks are transplanted superficially (surface transplantation) just beneath the puparium in early prepupal hosts, they evaginate synchronously with the host's organs and differentiate normally like the disks developing in situ. Histological evidence indicates that cells from the peripheral region of the disks migrate and join the epidermis of the host. This facilitates continuation of morphogenetic events culminating in eversion of the transplant. The significance of the cellular contact with epidermal cells of the host is discussed.A well developed nerve from the transplanted leg joins the thoraco-abdominal ganglion of the host. Flexion of the terminal leg segments indicates the presence of motor fibers innervating muscles in the leg.Disks from late third instar larvae and young prepupae evaginate whereas disks from younger third instar larvae fail to evaginate when surface transplanted. Disks, from all types of donors, cultured in adult abdomens fail to evaginate when retransplanted in prepupae. These and other results suggest that hormones may induce or inhibit morphogenetic cell movement in imaginal disks.The classical method of transplanting imaginal disks in Diptera, described by Ephrussi and Beadle ('36), has since been used extensively in investigations of various aspects of growth, determination and differentiation of these imaginal organ rudiments (see the reviews by Bodenstein, '53; Hadorn, '66; Ursprung, '66). In this method the disks are deposited within the body cavity of the host using a microinjection assembly. Disks thus transplanted in larval hosts metamorphose simultaneously with the hosts, but do not evaginate. The various segments remain telescoped within the peripodial sac and are difficult to identify except by microscopical examination of the cuticular pattern.Recently, we discovered a simple method of transplanting the disks in the prepupal stage which results in evagination of the organs as in normal development. The everted organ is in cellular contact with the epidermis and also develops nerve connection to the ganglion of the host. The significance of the epidermal contact is illustrated by the inhibition of disk development caused by irradiated epidermal cells (Bhaskaran and Sivasubramanian, '69). This system is therefore of great value in investigations of certain aspects of developmental biology and neurophysiology of insects. The present paper deals with a description of the technique and the structure of evaginated transplants. We have also presented some experimental evidence indicating the possible role of ecdysone in triggering morphogenetic cell movements in imaginal disks. MATERIAL AND METHODSThe Indian housefly Musca domesticu nebulo (Fabr) was used. Imaginal disks were dissected out in sterile ringer's solution (Ephrussi and Beadle, '36). With a razor blade the donor larva was cut just behind the ganglionic mass and a second time behind the mouth hook. All the leg disks ar...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
Contact Info
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Product
Resources
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.
