Development of truncated microtriches inEchinococcus granulosus protoscolices

Casado, N.; Pérez-Serrano, Jorge; Denegri, G.; Rodrı́guez-Caabeiro, F.

doi:10.1007/bf02351880

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…Hooks appeared as complete circles or half circles according to the position of the protoscoleces during examination. These observations were similar to those made by Casado et al [24] . Calcareous corpuscles in the protoscoleces are unique structures [25] .…”

Section: Discussionsupporting

confidence: 93%

Nitric oxide synthase: A potential factor in loss of hydatid protoscoleces viability via its role in cholesterol immunomodulation

Shaheen

Hamed

Abdeltawab

2022

Parasitologists United Journal

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Section: Discussionsupporting

confidence: 93%

Nitric oxide synthase: A potential factor in loss of hydatid protoscoleces viability via its role in cholesterol immunomodulation

Shaheen

Hamed

Abdeltawab

2022

Parasitologists United Journal

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“…0X05Y and might be due to the absence of the stabilizer DIP-PAcBA. The damage caused to the parasites by isoprinosine was essentially restricted to the occurrence of shorter microtriches, as reported by Casado et al (1994) with ivermectin. The vacuolization observed in vivo and with E. granulosus (Sarciron et al, 1995) using a longer duration of treatment was not seen in our tests.…”

Section: Discussionsupporting

confidence: 63%

In vitro effects of isoprinosine and a dipeptide methyl ester on Echinococcus multilocularis protoscoleces

Lawton¹,

Walchshofer²,

Sarciron³

2001

J. Helminthol.

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A protoscoleces/vesicles in vitro maintenance test with assessment of viability by eosin exclusion was used to evaluate the quantitative and qualitative activities of isoprinosine, its active component inosine and the dipeptide methylester L-Phe-Phe-OMe on isolated protoscoleces of Echinococcus multilocularis for 24 and 48 h. Isoprinosine and inosine showed dose- and time-dependent activity, the latter displaying a more rapid effect than the former. A high activity was shown with L-Phe-Phe-OMe, when compared to praziquantel. Ultrastructural alterations were much more striking with L-Phe-Phe-OMe, with an effect similar to that of praziquantel, whereas the chemotherapeutic activity of inosine and isoprinosine appeared to be directed against a metabolic target, with a lethal effect not immediately visible at the ultrastructural level. Thus, the previously reported in vivo activities of these drugs result largely from a direct effect on the parasite.

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“…In a first series of experiments, the morphological effects of in vitro treatment of E. multilocularis metacestodes in the presence of 10 g of either ABZSO or ABZSN per ml were observed. This drug concentration has been previously used to investigate morphological and ultrastructural alterations in E. granulosus protoscoleces (8,30). During the first 3 days of incubation, vesicles in all cultures remained intact, with no loss of turgidity.…”

Section: Resultsmentioning

confidence: 99%

Efficacies of Albendazole Sulfoxide and Albendazole Sulfone against In Vitro-Cultivated Echinococcus multilocularis Metacestodes

Ingold

Bigler²,

Thormann

et al. 1999

Antimicrob Agents Chemother

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The metacestode stage of Echinococcus multilocularis is the causative agent of alveolar echinococcosis (AE), a parasitic disease affecting the liver, with occasional metastasis into other organs. Benzimidazole carbamate derivatives such as mebendazole and albendazole are currently used for chemotherapeutic treatment of AE. Albendazole is poorly resorbed and is metabolically converted to its main metabolite albendazole sulfoxide, which is believed to be the active component, and further to albendazole sulfone. Chemotherapy with albendazole has been shown to have a parasitostatic rather than a parasitocidal effect; it is not effective in all cases, and the recurrence rate is rather high once chemotherapy is stopped. Thus, development of new means of chemotherapy of AE is needed. This could include modifications of benzimidazoles and elucidiation of the respective biological pathways. In this study we performed in vitro drug treatment of E. multilocularis metacestodes with albendazole sulfoxide and albendazole sulfone. High-performance liquid chromatography analysis of vesicle fluids showed that the drugs were taken up rapidly by the parasite. Transmission electron microscopic investigation of parasite tissues and nuclear magnetic resonance spectroscopy of vesicle fluids demonstrated that albendazole sulfoxide and albendazole sulfone had similar effects with respect to parasite ultrastructure and changes in metabolites in vesicle fluids. This study shows that the in vitro cultivation model presented here provides an ideal first-round test system for screening of antiparasite drugs.

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Development of truncated microtriches inEchinococcus granulosus protoscolices

Cited by 8 publications

References 13 publications

Nitric oxide synthase: A potential factor in loss of hydatid protoscoleces viability via its role in cholesterol immunomodulation

Nitric oxide synthase: A potential factor in loss of hydatid protoscoleces viability via its role in cholesterol immunomodulation

In vitro effects of isoprinosine and a dipeptide methyl ester on Echinococcus multilocularis protoscoleces

Efficacies of Albendazole Sulfoxide and Albendazole Sulfone against In Vitro-Cultivated Echinococcus multilocularis Metacestodes

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