Development of ultrasound-assisted emulsification microextraction for the determination of seven pesticides in apple juice by gas chromatography–mass spectrometry

Kaveli, Ioanna P.; Sakkas, Vasilios A.; Albanis, Triantafyllos A.

doi:10.1080/03067319.2014.994618

Cited by 5 publications

(1 citation statement)

References 29 publications

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“…Microorganisms must adapt to the environment to exhibit efficient metabolism, which in turn depends on access to nutritional sources and the possibility of their use. The residues used in this study could contain substances that can limit the metabolism of PAB (e.g., pesticides, heavy metals, and polyphenols) (Kiczorowska et al 2006 ; Góralczyk et al 2009 ; Kaveli et al 2015 ; Li et al 2015 , 2020 ). Apart from glycerol, waste glycerine contains methanol and free fatty acids (Pawlicka-Kaczorowska and Czaczyk 2017 ), which may limit the metabolism of microorganisms, including their uptake of nutrients from the growth environment.…”

Section: Discussionmentioning

confidence: 99%

Use of apple pomace, glycerine, and potato wastewater for the production of propionic acid and vitamin B12

Piwowarek

Lipińska

Hać‐Szymańczuk

et al. 2022

Appl Microbiol Biotechnol

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Propionic acid bacteria (PAB) are a source of valuable metabolites, including propionic acid and vitamin B12. Propionic acid, a food preservative, is synthesized from petroleum refining by-products, giving rise to ecological concerns. Due to changing food trends, the demand for vitamin B12 has been expected to increase in the future. Therefore, it is necessary to look for new, alternative methods of obtaining these compounds. This study was conducted with an aim of optimizing the production of PAB metabolites using only residues (apple pomace, waste glycerine, and potato wastewater), without any enzymatic or chemical pretreatment and enrichment. Media consisting of one, two, or three industrial side-streams were used for the production of PAB metabolites. The highest production of propionic acid was observed in the medium containing all three residues (8.15 g/L, yield: 0.48 g/g). In the same medium, the highest production of acetic acid was found — 2.31 g/L (0.13 g/g). The presence of waste glycerine in the media had a positive effect on the efficiency of propionic acid production and P/A ratio. The concentration of vitamin B12 obtained in the wet biomass of Propionibacterium freudenreichii DSM 20271 ranged from 90 to 290 µg/100 g. The highest production of cobalamin was achieved in potato wastewater and apple pomace, which may be a source of the precursors of vitamin B12 — cobalt and riboflavin. The results obtained show both propionic acid and vitamin B12 can be produced in a more sustainable manner through the fermentation of residues which are often not properly managed. Key points • The tested strain has been showed metabolic activity in the analyzed industrial side-streams (apple pomace, waste glycerine, potato wastewater). • All the side-streams were relevant for the production of propinic acid. • The addition of waste glycerine increases the propionic acid production efficiency and P/A ratio. • B12 was produced the most in the media containing potato wastewater and apple pomace as dominant ingredients.

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