Development of Unique House Mouse Resources Suitable for Evolutionary Studies of Speciation

Piálek, Jaroslav; Vyskočilová, Martina; Bímová, Barbora Vošlajerová; Havelková, Dana; Pialkova, Jana; Dufková, Petra; Bencova, Vera; Ďureje, Ľudovít; Albrecht, Tomáš; Hauffe, Heidi C.; Macholán, Miloš; Munclinger, Pavel; Storchová, Radka; Zajı́cová, Alena; Holáň, Vladimı́r; Gregorová, Soňa; Forejt, Jiřı́

doi:10.1093/jhered/esm083

Cited by 66 publications

(94 citation statements)

References 43 publications

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“…F 1 hybrid sterility F 1 hybrid male sterility is polymorphic among strains of M. m. musculus and M. m. domesticus (Vyskočilová et al 2005(Vyskočilová et al , 2009Good et al 2008b;Piálek et al 2008) and has not been evaluated in crosses between the wild-derived inbred strains PWD/PhJ (M. m. musculus PWD ) and WSB/EiJ (M. m. domesticus WSB ). To determine if F 1 males were sterile in crosses between these strains, we quantified a range of phenotypes in 70-day-old F 1 and parental males.…”

Section: Resultsmentioning

confidence: 99%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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Reproductive isolation between species is often caused by deleterious interactions among loci in hybrids. Finding the genes involved in these incompatibilities provides insight into the mechanisms of speciation. With recently diverged subspecies, house mice provide a powerful system for understanding the genetics of reproductive isolation early in the speciation process. Although previous studies have yielded important clues about the genetics of hybrid male sterility in house mice, they have been restricted to F 1 sterility or incompatibilities involving the X chromosome. To provide a more complete characterization of this key reproductive barrier, we conducted an F 2 intercross between wild-derived inbred strains from two subspecies of house mice, Mus musculus musculus and Mus musculus domesticus. We identified a suite of autosomal and X-linked QTL that underlie measures of hybrid male sterility, including testis weight, sperm density, and sperm morphology. In many cases, the autosomal loci were unique to a specific sterility trait and exhibited an effect only when homozygous, underscoring the importance of examining reproductive barriers beyond the F 1 generation. We also found novel two-locus incompatibilities between the M. m. musculus X chromosome and M. m. domesticus autosomal alleles. Our results reveal a complex genetic architecture for hybrid male sterility and suggest a prominent role for reproductive barriers in advanced generations in maintaining subspecies integrity in house mice.

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Section: Resultsmentioning

confidence: 99%

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

et al. 2011

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“…Fertility in F 1 hybrids has only been evaluated in a handful of inbred and wild representatives of M. m. castaneus, and F 1 hybrid male sterility is known to be polymorphic among strains of M. m. musculus and M. m. domesticus (Vyskočilová et al 2005(Vyskočilová et al , 2009Good et al 2008b;Piálek et al 2008). Furthermore, inbreeding depression in the parental strains might mask reduced fertility in the F 1 's.…”

Section: Discussionmentioning

confidence: 99%

Genetics and Evolution of Hybrid Male Sterility in House Mice

White¹,

Stubbings

Dumont³

et al. 2012

Genetics

105

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Comparative genetic mapping provides insights into the evolution of the reproductive barriers that separate closely related species. This approach has been used to document the accumulation of reproductive incompatibilities over time, but has only been applied to a few taxa. House mice offer a powerful system to reconstruct the evolution of reproductive isolation between multiple subspecies pairs. However, studies of the primary reproductive barrier in house mice-hybrid male sterility-have been restricted to a single subspecies pair: Mus musculus musculus and Mus musculus domesticus. To provide a more complete characterization of reproductive isolation in house mice, we conducted an F 2 intercross between wild-derived inbred strains from Mus musculus castaneus and M. m. domesticus. We identified autosomal and X-linked QTL associated with a range of hybrid male sterility phenotypes, including testis weight, sperm density, and sperm morphology. The pseudoautosomal region (PAR) was strongly associated with hybrid sterility phenotypes when heterozygous. We compared QTL found in this cross with QTL identified in a previous F 2 intercross between M. m. musculus and M. m. domesticus and found three shared autosomal QTL. Most QTL were not shared, demonstrating that the genetic basis of hybrid male sterility largely differs between these closely related subspecies pairs. These results lay the groundwork for identifying genes responsible for the early stages of speciation in house mice.

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“…Three of them (Stlt, StrA, StrB [39]) are derived from M. m. domesticus and were obtained from the breeding facility in Studenec (Czech Republic). Their average ages at dissection were 21 weeks (ranging from 10 to 72 weeks), 23 weeks (ranging from 16 to 45 weeks) and 20 weeks (ranging from 9 to 33 weeks), respectively.…”

Section: Methodsmentioning

confidence: 99%

Micro-evolutionary divergence patterns of mandible shapes in wild house mouse (Mus musculus) populations

Boell

Tautz

2011

BMC Evol Biol

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BackgroundInsights into the micro-evolutionary patterns of morphological traits require an assessment of the natural variation of the trait within and between populations and closely related species. The mouse mandible is a particularly suitable morphological trait for such an analysis, since it has long been used as a model to study the quantitative genetics of shape. In addition, many distinct populations, sub-species and closely related species are known for the house mouse. However, morphological comparisons among wild caught animals require an assessment in how far environmental and technical factors could interfere with the shape change measurements.ResultsUsing geometric morphometrics, we have surveyed mandible shapes in 15 natural populations of the genus Mus, with a focus on the subspecies Mus musculus domesticus. In parallel we have carefully assessed possibly confounding technical and biological factors. We find that there are distinct differences on average between populations, subspecies and species, but these differences are smaller than differences between individuals within populations. Populations from summer-dry regions, although more ancestral, are less distinct from each other than are populations from the more recently colonized northern areas. Populations with especially distinct shapes occur in an area of sympatry of M. m. domesticus and M. spretus and on recently colonized sub-antarctic islands. We have also studied a number of inbred strains to assess in how far their mandible shapes resemble those from the wild. We find that they fall indeed into the shape space of natural variation between individuals in populations.ConclusionsAlthough mandible shapes in natural populations can be influenced by environmental variables, these influences are insufficient to explain the average extent of shape differences between populations, such that evolutionary processes must be invoked to explain this level of diversity. We discuss that adaptive evolution may contribute to shape changes between populations, in particular in newly colonized areas. A comparison between inbred strains and wild mice suggests that the laboratory environment has no major systematic effect on the mandible shape and that such strains can be used as representatives of the natural shape differences between individuals.

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Development of Unique House Mouse Resources Suitable for Evolutionary Studies of Speciation

Cited by 66 publications

References 43 publications

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Genetic Dissection of a Key Reproductive Barrier Between Nascent Species of House Mice

Genetics and Evolution of Hybrid Male Sterility in House Mice

Micro-evolutionary divergence patterns of mandible shapes in wild house mouse (Mus musculus) populations

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