Development of validated determination of the eleven marker compounds in Gyejibokryeong-hwan for the quality assessment using HPLC analysis

Seo, Chang‐Seob; Shin, Hyeun‐Kyoo

doi:10.1007/s12272-014-0363-z

Cited by 18 publications

(12 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GBH has been used extensively to treat blood stasis and climacteric syndrome throughout Asia [ 11 , 12 ] and has been approved by both the Korean Ministry of Food and Drug Safety (MFDS) and the US Food and Drug Administration (FDA). Additional studies have investigated the safety and efficacy of GBH in various populations [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression

Park

Kim

et al. 2018

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Treatment with the antihypertensive agent reserpine depletes monoamine levels, resulting in depression. In the present study, we evaluated the antidepressant effects of Gyejibokryeong-hwan (GBH), a traditional Korean medicine, in a mouse model of reserpine-induced depression. Mice were treated with reserpine (0.5 mg·kg−1, i.p.) or phosphate-buffered saline (PBS, i.p., normal) once daily for 10 days. GBH (50, 100, 300, and 500 mg·kg−1), PBS (normal, control), fluoxetine (FXT, 20 mg·kg−1), or amitriptyline (AMT, 30 mg·kg−1) was administered orally 1 h prior to reserpine treatment. Mouse behavior was examined in the forced swim test (FST), tail suspension test (TST), and open-field test (OFT) following completion of the treatment protocol. Administration of GBH reduced immobility time in the FST and TST and significantly increased the total distance traveled in the OFT. Plasma serotonin levels were significantly lower in control mice than in normal mice, although these decreases were significantly attenuated to a similar extent by treatment with GBH, FXT, or AMT. Reserpine-induced increases in plasma corticosterone were also attenuated by GBH treatment. Moreover, GBH attenuated reserpine-induced increases in interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α mRNA expression in the hippocampus. In addition, GBH mice exhibited increased levels of brain-derived neurotrophic factor (BDNF) and a higher ratio of phosphorylated cAMP response element-binding protein (p-CREB) to CREB (p-CREB/CREB) in the hippocampus. Our results indicated that GBH can ameliorate depressive-like behaviors, affect the concentration of mood-related hormones, and help to regulate immune/endocrine dysfunction in mice with reserpine-induced depression, likely via activation of the BDNF-CREB pathway. Taken together, these findings indicate that GBH may be effective in treating patients with depression.

show abstract

Section: Introductionmentioning

confidence: 99%

Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression

Park

Kim

et al. 2018

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although quantitative analysis using the UPLC method has been widely used for the quality assessment of herbal medicine or preparation, 12 gas chromatography or HPLC using sulfuric acid as the mobile phase has been generally used for the quantification of propionic acid content. The developed method from this study using UPLC was successfully applied to the measurement of S. baicalensis roots, showing shorter analysis time, more convenient conditions, and lower LOD and LOQ values.…”

Section: Discussionmentioning

confidence: 99%

Quantification of propionic acid from Scutellaria baicalensis roots

Son

Kim

et al. 2017

Integrative Medicine Research

View full text Add to dashboard Cite

BackgroundPropionic acid is a widely used preservative and has been mainly formed by artificial synthesis or fermentation. In the case of natural products, the presence of propionic acid is viewed as a sign that an additive has been introduced for antimicrobial effects.MethodsIn this work, the propionic acid that occurs in Scutellaria baicalensis roots was studied. A quantification method was developed, validated, and showed good linearity, low limit of detection, and limit of quantification values, as well as fine precision and recovery rate. The developed method was applied to the analysis of S. baicalensis roots collected in South Korea and China.ResultsThe detection rate for all samples was 94.0%. The average concentration was 0.41 ± 0.24 mg/g from the China sample and 0.76 ± 0.28 mg/g from the Korea sample.ConclusionThis study is the first to report that propionic acid exists in S. baicalensis roots and also provides a useful ultra performance liquid chromatography analysis method for its quantification.

show abstract

“…The HPLC chemical fingerprint is the technique that is mostly used as a chemical method for authentication of HMP [214]. However, despite its wide acceptance, it has a number of limitations.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Abubakar

Salleh²,

Shamsir³

et al. 2017

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

In the last two decades, there has been a tremendous increase in the global use of herbal medicinal products (HMPs) due to their claimed health benefits. This has led to increase in their demand and consequently, also, resulted in massive adulteration. This is due to the fact that most of the traditional methods cannot identify closely related species in a process product form. Therefore the urgent need for simple and rapid identification methods resulted in the discovery of a novel technique. DNA barcoding is a process that uses short DNA sequence from the standard genome for species identification. This technique is reliable and is not affected by external factors such as climates, age, or plant part. The difficulties in isolation of DNA of high quality in addition to other factors are among the challenges encountered using the DNA barcoding in the authentication of HMP. These limitations indicated that using DNA barcoding alone may ineffectively authenticate the HMP. Therefore, the combination of DNA barcoding with chromatographic fingerprint, a popular and generally accepted technique for the assessment and quality control of HMP, will offer an efficient solution to effectively evaluate the authenticity and quality consistency of HMP. Detailed and quality information about the main composition of the HMPs will help to ascertain their efficacy and safety as these are very important for quality control.

show abstract

Development of validated determination of the eleven marker compounds in Gyejibokryeong-hwan for the quality assessment using HPLC analysis

Cited by 18 publications

References 18 publications

Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression

Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression

Quantification of propionic acid from Scutellaria baicalensis roots

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Contact Info

Product

Resources

About