Development of Xanthophyllomyces dendrorhous as a production system for the colorless carotene phytoene

Pollmann, Hendrik; Breitenbach, Jürgen; Sandmann, Gerhard

doi:10.1016/j.jbiotec.2017.02.027

Cited by 19 publications

(14 citation statements)

References 26 publications

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“…3C). In spite of a natural carotenoid-producing strain, the titer is still less than that of previous studies reporting phytoene production using eukaryotic cells [5,8,29] and non-natural producers [6,30]. We hypothesized that this is mainly because of low cell density.…”

Section: Enhanced Production Of Phytoene By Deletion Of the Crtd Genementioning

confidence: 90%

“…More recently, geneticallyengineered Dunaliella salina V-101 capable of producing phytoene up to 108.34 mg/ 100 mg DCW was developed by down-regulation of phytoene desaturase using RNAi and antisense methods [5]. Another study also reported the highest level of phytoene production (up to 10 mg/g DCW) using Xanthophyllomyces dendrorhous by deleting the crtI gene together with overexpressing the crtE and crtYB genes [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene

Jeong

Kang

Choi

2018

Journal of Microbiology and Biotechnology

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A metabolically-engineered Deinococcus radiodurans R1 strain capable of producing phytoene, a colorless C 4 0 carotenoid and a promising antioxidant, has been developed. To make this base strain, first, the crtI gene encoding phytoene desaturase was deleted to block the conversion of phytoene to other carotenoids such as lycopene and γ-carotene. This engineered strain produced 0.413 ± 0.023 mg/l of phytoene from 10 g/l of fructose. Further enhanced production of phytoene up to 4.46 ± 0.19 mg/l was achieved by overexpressing the crtB gene encoding phytoene synthase and the dxs genes encoding 1-deoxy-D-xylulose-5-phosphate synthase gene, and by deleting the crtD gene. High cell-density culture of our final engineered strain allowed production of 10.3 ± 0.85 mg/l of phytoene with the yield and productivity of 1.04 ± 0.05 mg/g and 0.143 ± 0.012 mg/l/h, respectively, from 10 g/l of fructose. Furthermore, the antioxidant potential of phytoene produced by the final engineered strain was confirmed by in vitro DPPH radical-scavenging assay.

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Section: Enhanced Production Of Phytoene By Deletion Of the Crtd Genementioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene

Jeong

Kang

Choi

2018

Journal of Microbiology and Biotechnology

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“…Within the astaxanthin biosynthesis pathway, phytoene-β-carotene synthase ( crtYB ) is also a bottleneck for the accumulation of astaxanthin due to its low expression levels [ 33 , 55 – 58 ]. Similarly, low expression levels of cytochrome P450 reductase ( crtR ), which participates in the catalysis of the last step of the astaxanthin biosynthesis, have been observed [ 51 , 56 , 59 – 61 ].…”

Section: Biosynthesis Of Astaxanthin In X Dendrorhousmentioning

confidence: 99%

“…A Representation of knock-in and knock-out strategies for genetic engineering in yeast. B Overexpression of HMG1 , crtE and crtYB for high production of phytoene [ 58 ]. C Genetic engineering of X. dendrorhous for high yield astaxanthin production.…”

Section: Metabolic Engineering Of X Dendrorhousmentioning

confidence: 99%

Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous

et al. 2021

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Astaxanthin is a carotenoid with a number of assets useful for the food, cosmetic and pharmaceutical industries. Nowadays, it is mainly produced by chemical synthesis. However, the process leads to an enantiomeric mixture where the biologically assimilable forms (3R, 3′R or 3S, 3′S) are a minority. Microbial production of (3R, 3′R) astaxanthin by Xanthophyllomyces dendrorhous is an appealing alternative due to its fast growth rate and easy large-scale production. In order to increase X. dendrorhous astaxanthin yields, random mutant strains able to produce from 6 to 10 mg/g dry mass have been generated; nevertheless, they often are unstable. On the other hand, site-directed mutant strains have also been obtained, but they increase only the yield of non-astaxanthin carotenoids. In this review, we insightfully analyze the metabolic carbon flow converging in astaxanthin biosynthesis and, by integrating the biological features of X. dendrorhous with available metabolic, genomic, transcriptomic, and proteomic data, as well as the knowledge gained with random and site-directed mutants that lead to increased carotenoids yield, we propose new metabolic engineering targets to increase astaxanthin biosynthesis.

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“…Colorless phytoene and phytofluene, carotenoids precursors to lycopene, receive attentions in their nutritional aspects [12, 57]. Phytoene production can be attained by disrupting its desaturation step and yield of 7.5 mg/g DCW is reported in X. dendrorhous [58]. Rare C 50 carotenoids have longer c.d.b.…”

Section: Challenges and Outlook In Microbial Production Of Carotenoidsmentioning

confidence: 99%

Challenges and tackles in metabolic engineering for microbial production of carotenoids

et al. 2019

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Naturally occurring carotenoids have been isolated and used as colorants, antioxidants, nutrients, etc. in many fields. There is an ever-growing demand for carotenoids production. To comfort this, microbial production of carotenoids is an attractive alternative to current extraction from natural sources. This review summarizes the biosynthetic pathway of carotenoids and progresses in metabolic engineering of various microorganisms for carotenoid production. The advances in synthetic pathway and systems biology lead to many versatile engineering tools available to manipulate microorganisms. In this context, challenges and possible directions are also discussed to provide an insight of microbial engineering for improved production of carotenoids in the future.

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Development of Xanthophyllomyces dendrorhous as a production system for the colorless carotene phytoene

Cited by 19 publications

References 26 publications

Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene

Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene

Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous

Challenges and tackles in metabolic engineering for microbial production of carotenoids

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