Development of Zika NS1 ELISA methodology for seroprevalence detection in a cohort of Mexican patients in an endemic region

Kim, Young Chan; García-Larragoiti, Nallely; Cano-Méndez, Alan; Hernández-Flores, Karina Guadalupe; Domínguez-Alemán, Carlos Alonso; Cabrera-Jorge, Francisco Javier; Mar, María Antonieta; Vivanco-Cid, Héctor; Viveros-Sandoval, Martha Eva; Reyes-Sandoval, Arturo

doi:10.1016/j.jcvp.2021.100024

Cited by 1 publication

(2 citation statements)

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“…Therefore, tests have been developed for use in serum, usually with labeled antibodies for NS1 detection. Some of these tests have reduced cross-reactivity and high sensitivity [ 21 , 22 ].…”

Section: Conventional Methodologies For Direct Detection Of Flavivirusmentioning

confidence: 99%

“…Many studies have described homemade protocols that achieve better parameters than commercial tests [ 22 ] and rapid antigen-detection assays [ 24 ]. The vast majority of studies have focused on the diagnosis of dengue, evaluating cross-reactivity with ZIKV, which is especially important for serological discrimination in endemic regions with co-circulation of these viruses [ 25 ].…”

Section: Conventional Methodologies For Direct Detection Of Flavivirusmentioning

confidence: 99%

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Challenges in Direct Detection of Flaviviruses: A Review

et al. 2023

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Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology.

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Section: Conventional Methodologies For Direct Detection Of Flavivirusmentioning

confidence: 99%