Development, optimization, and <i>in vitro</i> characterization of dasatinib-loaded PEG functionalized chitosan capped gold nanoparticles using Box–Behnken experimental design

Adena, Sandeep Kumar Reddy; Upadhyay, Mansi; Vardhan, Harsh; Mishra, Brahmeshwar

doi:10.1080/03639045.2017.1402919

Cited by 23 publications

(8 citation statements)

References 26 publications

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“…It is mainly determined by the surface integrated compounds. For example, metallic nanoparticles with a surface charge of either more negative (<-30) or more positive (<+30) have depicted superior stability ( Adena et al., 2019 ). This study has noted the zeta charge of -19.5 mV for the synthesized AG-AuNPs ( Figure 5 d), illustrating their remarkable structural stability in the medium.…”

Section: Resultsmentioning

confidence: 99%

Apium graveolens reduced phytofabricated gold nanoparticles and their impacts on the glucose utilization pattern of the isolated rat hemidiaphragm

Rajasekar¹,

Mohan²,

Muniyan³

2022

Heliyon

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The integration of nanotechnology with herbal medicines overcomes the limitations of herbal therapy and offers desirable bioactivity. Thus, this study was aimed at synthesizing Apium graveolens -gold nanoparticles (AG-AuNPs) and exploring their impact on the glucose utilization pattern of the isolated rat hemidiaphragm. The AG-AuNPs appeared in cherry red color and showed a plasmonic peak at 534nm. The bio-reduced AG-AuNPs appeared as spherical shapes with varying sizes of about 4–15 nm. It also depicted the zeta potential of -19.5 mV, face-centered cubic crystalline nature, stretching vibrations for different functionalities, and in vitro stability during various characterization studies. The AG-AuNPs showed non-cytotoxicity and promoted cellular glucose uptake on their exposure to the cell line and the diaphragm, respectively. Moreover, the identified flavonoids and polyphenols of AG depicted in silico interactions with the insulin signaling molecule. The observed results suggest that the interacting ability of AG-AuNPs with the insulin signaling molecule can promote the glucose uptake efficiency of the diaphragm.

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Section: Resultsmentioning

confidence: 99%

Apium graveolens reduced phytofabricated gold nanoparticles and their impacts on the glucose utilization pattern of the isolated rat hemidiaphragm

Rajasekar¹,

Mohan²,

Muniyan³

2022

Heliyon

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“…All dependent variables were analyzed with the quadratic model, and 3D surface plots for responses were created. The quadratic model’s statistical study found significant p -values, indicating a better fit for selected responses ( Table 4 ) [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Novel Therapeutic Approach in PEGylated Chitosan Nanoparticles of Apigenin for the Treatment of Cancer via Oral Nanomedicine

et al. 2022

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The goal of this study was to optimize and formulate apigenin (APG)-loaded pegylated chitosan nanoparticles (PEGylated-CNPs) via ionic gelation techniques using the Box–Behnken design (BBD). Three individual variables, X1(chitosan: TPP concentration), X2 (PEG-400 concentration), and X3 (sonication time), were investigated for their influence on response variables (Y1—particle size (PS); Y2—drug entrapment efficiency (DEE); and Y3—zeta potential (ZP). The optimized formula of APG-PEGylated CNPs was picked from the statistical design and was then examined for physical, morphological, release characterization, anti-oxidant, and anti-tumor potential. The average PS, PDI, %DEE, and ZP were found to be 139.63 ± 5.67 nm, 0.296 ± 0.014, 79.55 ± 3.12%, and 24.68 ± 1.84 mV, respectively. The optimized APG formulation was chosen and reformulated based on the desirability function. Results of the observed and predicted values of responses through the BBD process were found to be nearly identical. The resulting APG-PEGylated CNPs were spherical and smooth, according to surface morphology studies. The release study revealed that PEGylated-CNPs exhibited biphasic release patterns distinguished by an initial burst release of APG only at early phases accompanied by a delayed release near 24 h. Furthermore, APG-PEGylated CNPs demonstrated statistically increased antioxidant activities and cytotoxicity against MCF-7 cells compared to pure APG. Based on the findings, it is possible to conclude that BBD was efficient in optimizing the PEGylated CNPs formulation and recognizing the impacts of formulation variables. In conclusion, the developed formulation has a significant potential for anticancer therapy.

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“…The same authors also reported a new process for the preparation of AuNPs using chitosan and PEG coating of the particle surface, and entrapping Dasatinib in the chitosan–PEG surface corona. In this way, they obtained Dasatinib-loaded PEG-stabilized CS-capped AuNPs stable at different storage conditions and endowed with sustained drug release of up to 76% in 48 h [ 114 ].…”

Section: Nanoparticles Of Tyrosine Kinase Inhibitorsmentioning

confidence: 99%

Nanotechnology of Tyrosine Kinase Inhibitors in Cancer Therapy: A Perspective

Russo

Spallarossa

Tasso

et al. 2021

IJMS

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Nanotechnology is an important application in modern cancer therapy. In comparison with conventional drug formulations, nanoparticles ensure better penetration into the tumor mass by exploiting the enhanced permeability and retention effect, longer blood circulation times by a reduced renal excretion and a decrease in side effects and drug accumulation in healthy tissues. The most significant classes of nanoparticles (i.e., liposomes, inorganic and organic nanoparticles) are here discussed with a particular focus on their use as delivery systems for small molecule tyrosine kinase inhibitors (TKIs). A number of these new compounds (e.g., Imatinib, Dasatinib, Ponatinib) have been approved as first-line therapy in different cancer types but their clinical use is limited by poor solubility and oral bioavailability. Consequently, new nanoparticle systems are necessary to ameliorate formulations and reduce toxicity. In this review, some of the most important TKIs are reported, focusing on ongoing clinical studies, and the recent drug delivery systems for these molecules are investigated.

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Development, optimization, and in vitro characterization of dasatinib-loaded PEG functionalized chitosan capped gold nanoparticles using Box–Behnken experimental design

Abstract: A process for preparing gold nanoparticles using chitosan, anchoring PEG to the particle surface, and entrapping dasatinib in the chitosan-PEG surface corona was optimized.

Cited by 23 publications

References 26 publications

Apium graveolens reduced phytofabricated gold nanoparticles and their impacts on the glucose utilization pattern of the isolated rat hemidiaphragm

Apium graveolens reduced phytofabricated gold nanoparticles and their impacts on the glucose utilization pattern of the isolated rat hemidiaphragm

Novel Therapeutic Approach in PEGylated Chitosan Nanoparticles of Apigenin for the Treatment of Cancer via Oral Nanomedicine

Nanotechnology of Tyrosine Kinase Inhibitors in Cancer Therapy: A Perspective

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