Development, optimization and validation of a multimethod for the determination of 36 mycotoxins in wines by liquid chromatography–tandem mass spectrometry

Pizzutti, Ionara Regina; Kok, Albert; Scholten, Jos; Righi, Laís W.; Cardoso, Carmem D.; Rohers, Graciele Necchi; Silva, Rosselei C. da

doi:10.1016/j.talanta.2014.05.017

Cited by 74 publications

(35 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous publications have already dealt with QuEChERS-based extraction for determination of mycotoxins in various types of samples. Pizzutti et al developed, optimized and validated a multiresidue method for the determination of 36 mycotoxins in wines by LC-MS/ MS [77]. Multi-mycotoxin analysis in beer-based drinks by a modified QuEChERS method and UHPLC-MS/MS was conducted by Tamura et al [78].…”

Section: Mycotoxins Analysismentioning

confidence: 99%

A review of recent developments and trends in the QuEChERS sample preparation approach

2015

View full text Add to dashboard Cite

Abstract:A comprehensive review is presented on the recent developments and trends in the QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation approach. This technique involves liquidliquid partitioning using acetonitrile and purifying the extract using dispersive solid-phase extraction (d-SPE). Originally, the QuEChERS was introduced for pesticides residues analysis in high moisture fruits and vegetables, but more recently it is gaining significant popularity in the analysis of broad spectrum of analytes in huge variety of samples. The wide range of the technique applications is possible due to introducing various modifications based on the use of different extraction solvent and salt formulation and buffer additions for salting-out partitioning step and the application of various d-SPE sorbents for clean-up step. Therefore, the QuEChERS approach is useful for analysis of, among others pesticides, veterinary drugs and other pharmaceuticals, mycotoxins, polycyclic aromatic hydrocarbons (PAHs), dyes, acrylamide, synthetic musks and UV filters, bisphenols, polybrominated diphenyl ethers and other flame retardants, endocrine disruptors, and other chemical compounds. Thanks to the QuEChERS approach, high-throughput multiresidue methods operate in a routine contaminant control of food products, feedstuff, and environmental samples.

show abstract

Section: Mycotoxins Analysismentioning

confidence: 99%

A review of recent developments and trends in the QuEChERS sample preparation approach

2015

View full text Add to dashboard Cite

show abstract

“…For instance, Bertuzzi et al (2015) found MPA both in fresh chestnuts and chestnut flour (100 % positive; concentration range 7-850 μg/kg). Contamination of wine samples with MPA was reported by Meyer et al (2001) and Pizzutti et al (2014); maximum toxin concentrations found were 130 and 54 μg/ kg, respectively. Moreover, 16 % of analysed dietary supplements containing green coffee bean extracts were MPA positive in concentrations of up to 395 μg/kg (Vaclavik et al 2013).…”

Section: Introductionmentioning

confidence: 93%

“…In general, recoveries were in the range from 74 to 78 %; higher values were found for the 0.1-ng/g spiking level, indicating a slight bias of the analyses due to residual matrix components. With a detection limit of <0.1 ng/g, the developed antibody-based analytical approach represents one of the most sensitive analysis method for this mycotoxin described so far (De Smet et al 2011;Kononenko et al 2012;Pizzutti et al 2014;Schneweis et al 2000;Sulyok et al 2010;Vaclavik et al 2013;Zachariasova et al 2014). Moreover, the established IA clean-up procedure could also improve the performance of LC-MS(/MS) methods which are often negatively influenced by intense signal suppression due to matrix components (De Smet et al 2011;Vaclavik et al 2013).…”

Section: Establishment Of the Ia Clean-upmentioning

confidence: 99%

“…Particularly in recent years, MPA analyses of food and feed were often conducted as part of multi-analyte approaches (Pizzutti et al 2014;Sulyok et al 2010;Vaclavik et al 2013;Van Pamel et al 2011;Zachariasova et al 2014), but as stated by De Smet et al (2011), most existing multianalytical methods lack sensitivity due to the absence of proper clean-up methods. As an alternative approach, sensitive immunochemical methods based on polyclonal rabbit antisera have been described by Usleber et al (2008) and Burkin and Kononenko (2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and application of monoclonal antibodies against the mycotoxin mycophenolic acid

Dietrich

Märtlbauer

2015

Mycotoxin Res

View full text Add to dashboard Cite

Mycophenolic acid (MPA) is frequently found, often in high concentrations, in a broad range of food and feed matrices. Apart from the well-known contamination of blue-veined cheeses caused by the use of toxinogenic Penicillium roqueforti strains for manufacturing, a broad range of other Penicillium spp. is able to produce this immunosuppressive toxin. Therefore, MPA has been proposed to be a suitable marker for Penicillium-infected food commodities. In the present work, a high-affinity monoclonal antibody (mAb) for the specific detection of MPA was developed by immunizing mice with a MPA-protein conjugate coupled by an activated ester method. Under the conditions of a direct competitive enzyme immunoassay (EIA), 50% inhibition and detection limits of MPA standard curves were 1.2 and 0.3 ng/ml, respectively. Furthermore, the mAb could be successfully employed for the production of an immunoaffinity (IA) column enabling the efficient enrichment of MPA from processed foodstuffs. By combining the IA clean-up with a polyclonal antibody-based EIA, an ultrasensitive analysis method could be established which allowed the reliable and reproducible detection of MPA in artificially contaminated tomato ketchup as a model matrix at concentrations as low as 0.1 ng/g.

show abstract

“…Some analytes are more affected than others. 15,16 The matrix effect is the effect observed by the increase or decrease in detector response for a particular analyte present in the matrix extract compared to the detector response for the analyte in organic solvent. 17 In this study, the matrix effect was evaluated in two different ways.…”

Section: Matrix Effect Evaluationmentioning

confidence: 99%

Pesticide Residues Method Validation by UPLC-MS/MS for Accreditation Purposes

Pizzutti¹,

Dias²,

Kok³

et al. 2016

Journal of the Brazilian Chemical Society

Self Cite

View full text Add to dashboard Cite

The development and validation of an analytical method for the determination of 51 pesticides in tomato using UPLC-MS/MS (ultra performance liquid chromatography coupled to tandem mass spectrometry) was performed in order to fulfill the requirements of the NBR ISO/IEC 17025:2005. The goal of the validation was to evaluate all required parameters, such as linearity of analytical curves, instrument and method limits of detection and quantification, matrix effect and accuracy (trueness and precision). Stock solutions of the pesticides were prepared in organic solvent by two different analysts independently, in order to validate this preparation by comparing the detector response areas of injections (n = 7). Pesticides were extracted from tomatoes applying the Dutch mini-Luke method with 30 mL of each solvent as acetone, petroleum ether as well as dichloromethane. A small aliquot of the organic solvent extract was evaporated and reconstituted in methanol with 0.1% of acetic acid (v/v) for UPLC-MS/MS analysis. The recovery experiments were done by spiking blank samples at 10, 20 and 50 µg kg -1 , performed by two analysts, seven replicates for each concentration level plus the blank sample. From the 51 studied compounds, 46 showed recoveries within the acceptable range of 70-120% for all evaluated concentrations. For 82% of pesticides, the validated method limit of quantification was the lowest concentration studied (10 µg kg

show abstract

Development, optimization and validation of a multimethod for the determination of 36 mycotoxins in wines by liquid chromatography–tandem mass spectrometry

Cited by 74 publications

References 23 publications

A review of recent developments and trends in the QuEChERS sample preparation approach

A review of recent developments and trends in the QuEChERS sample preparation approach

Development and application of monoclonal antibodies against the mycotoxin mycophenolic acid

Pesticide Residues Method Validation by UPLC-MS/MS for Accreditation Purposes

Contact Info

Product

Resources

About