Development-promoting effect of chicken embryo membrane on chicken ovarian cortical pieces of different age

Abstract

Ovarian cortical tissues of various ages of chicken were grafted underneath chorioallantoic membrane (CAM) of 6-d-old chicken embryo and the grafts were collected on d 0, 2, 4, 8, or 10. The tissue sections were prepared to examine the development of follicles in grafts and the proliferative ability of follicles was examined by immunohistochemistry analysis of proliferating cell nuclear antigen. The results indicated that the development of follicles in cultured chicken ovarian cortical pieces progressed smoothly and slowly similar to those in vivo, and the environment of chicken embryo was more suitable for the synchronous development of oocyte and follicular cells in the larger follicles. Meanwhile, the synchronous development could be more easily achieved in the chicken ovarian tissues of 12- to 15-wk chicken. Neither oocyte nor granulosa cells but the thecal cells of follicles developed in grafts of 13- to 15-d chicken within 10 d of culturing in ovo, and the CAM can ease follicular atresia in chicken ovarian grafts of 12- to 15-wk chicken. The proliferative performance of follicles in the grafts was not influenced by the environment of chicken embryo. These results indicated that the chicken embryo has the ability to support slow development of primordial and primary follicles in grafted ovarian cortical tissues of chickens of different ages. The CAM system may also prove useful for the study of early follicle development with further information.

Early avian follicular development is characterized by changes in transcripts involved in steroidogenesis, paracrine signaling and transcription

“…Similarly, FOXL2 , WT1 and WNT4 decreased in ovo to similar levels as observed in vivo, while NR5A1 did not change in vivo or in ovo . The CAM culture system has been used successfully to grow pieces of ovarian cortex from cattle, mice and chickens (Cushman et al, 2002; Gigli et al, 2005; Yuan et al, 2009). Although both male and female embryos were used for CAM culture in the present and previous studies, there was not an obvious effect of embryo sex on transcript abundance as indicated by the low variation in transcript levels.…”

“…To study early development of avian follicles in culture, we adapted an in ovo system for the culture of ovarian cortical pieces previously used successfully with bovine, mouse and chickens ovarian cortical pieces (Cushman et al, 2002; Gigli et al, 2005; Yuan et al, 2009). Freshly laid, fertile chicken eggs (White leghorn) were collected and incubated in a rotating egg incubator for 3 days.…”

Chronic Heat Stress Damages Small Intestinal Epithelium Cells Associated with the Adenosine 5′-Monophosphate-Activated Protein Kinase Pathway in Broilers

“…Proliferating Cell Nuclear Antigen (PCNA) Immunohistochemistry. PCNA-positive cells were determined using the anti-PCNA of mouse monoclonal antibodies (BM0104, Boster, Wuhan, China), 22 employing a diaminobenzidine (DAB) staining kit (K5007, Angle Gene Bioengineering Co., Ltd., Nanjing, China), with reference to the instructions of the manufacturer. Once immunohistochemically stained, the duodenal and jejunal sections were captured by employing a DP12 charge-coupled device (CCD) digital camera (Scope.A1, Carl Zeiss, Germany) with a final magnification of 400×.…”

In ovo feeding of l‐arginine regulates intestinal barrier functions of posthatch broilers by activating the mTOR signaling pathway

“…Proliferating cell nuclear antigen (PCNA)‐positive cells were measured by use of mouse monoclonal anti‐PCNA antibody (BM0104; Boster, Wuhan, China), followed by the use of a diaminobenzidine (DAB) staining kit (K5007; Angle Gene Bioengineering Co. Ltd), according to the manufacturer's directions. After immunohistochemical staining, the jejunal sections of 21‐day‐old broilers were captured using an Olympus DP12 CCD digital camera with a final magnification of ×400.…”