Development under Constant Temperatures and Seasonal Prevalence in Soybean Field of the Bean Pyralid, Omiodes indicatus (Lepidoptera: Crambidae)

Choi, K. M.; Hong, Yong Deog; Chang, Young-Jik; Moon, J.Y.; Kim, Chi-Sun; Choi, Dong-Chil; Kim, Tae‐Heung

doi:10.5656/ksae.2008.47.4.353

Cited by 5 publications

(2 citation statements)

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“…Bean pyralid (Lamprosema indicata (Fabricius)) is an important leaf-feeding insect, is widely distributed throughout the world and is found in China, Korea, Japan, India, the Americas and Africa [1]. During the years when serious damage has occurred, it can produce 4 to 5 generations a year, with overlapping of generations in the soybean producing areas.…”

Section: Introductionmentioning

confidence: 99%

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

et al. 2021

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Background Bean pyralid is one of the major leaf-feeding insects that affect soybean crops. DNA methylation can control the networks of gene expressions, and it plays an important role in responses to biotic stress. However, at present the genome-wide DNA methylation profile of the soybean resistance to bean pyralid has not been reported so far. Results Using whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq), we analyzed the highly resistant material (Gantai-2-2, HRK) and highly susceptible material (Wan82–178, HSK), under bean pyralid larvae feeding 0 h and 48 h, to clarify the molecular mechanism of the soybean resistance and explore its insect-resistant genes. We identified 2194, 6872, 39,704 and 40,018 differentially methylated regions (DMRs), as well as 497, 1594, 9596 and 9554 differentially methylated genes (DMGs) in the HRK0/HRK48, HSK0/HSK48, HSK0/HRK0 and HSK48/HRK48 comparisons, respectively. Through the analysis of global methylation and transcription, 265 differentially expressed genes (DEGs) were negatively correlated with DMGs, there were 34, 49, 141 and 116 negatively correlated genes in the HRK0/HRK48, HSK0/HSK48, HSK0/HRK0 and HSK48/HRK48, respectively. The MapMan cluster analysis showed that 114 negatively correlated genes were clustered in 24 pathways, such as protein biosynthesis and modification; primary metabolism; secondary metabolism; cell cycle, cell structure and component; RNA biosynthesis and processing, and so on. Moreover, CRK40; CRK62; STK; MAPK9; L-type lectin-domain containing receptor kinase VIII.2; CesA; CSI1; fimbrin-1; KIN-14B; KIN-14 N; KIN-4A; cytochrome P450 81E8; BEE1; ERF; bHLH25; bHLH79; GATA26, were likely regulatory genes involved in the soybean responses to bean pyralid larvae. Finally, 5 DMRs were further validated that the genome-wide DNA data were reliable through PS-PCR and 5 DEGs were confirmed the relationship between DNA methylation and gene expression by qRT-PCR. The results showed an excellent agreement with deep sequencing. Conclusions Genome-wide DNA methylation profile of soybean response to bean pyralid was obtained for the first time. Several specific DMGs which participated in protein kinase, cell and organelle, flavonoid biosynthesis and transcription factor were further identified to be likely associated with soybean response to bean pyralid. Our data will provide better understanding of DNA methylation alteration and their potential role in soybean insect resistance.

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Section: Introductionmentioning

confidence: 99%

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

et al. 2021

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“…Bean pyralid ( Lamprosema indicata Fabricius) is an important leaf-feeding pest of soybean, it is widely distributed throughout the world and is found in Korea, Japan, China, India, the Americas, and Africa [15]. It can only feed and reproduce on soybean, so soybean is its only food source.…”

Section: Introductionmentioning

confidence: 99%

Determination of the MiRNAs Related to Bean Pyralid Larvae Resistance in Soybean Using Small RNA and Transcriptome Sequencing

Zeng

Sun

Lai

et al. 2019

IJMS

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Soybean is one of the most important oil crops in the world. Bean pyralid is a major leaf-feeding insect of soybean. In order to screen out the functional genes and regulatory pathways related to the resistance for bean pyralid larvae, the small RNA and transcriptome sequencing were performed based on the highly resistant material (Gantai-2-2) and highly susceptible material (Wan 82-178) of soybean. The results showed that, when comparing 48 h feeding with 0 h feeding, 55 differentially expressed miRNAs were identified in Gantai-2-2 and 58 differentially expressed miRNAs were identified in Wan82-178. When comparing Gantai-2-2 with Wan82-178, 77 differentially expressed miRNAs were identified at 0 h feeding, and 70 differentially expressed miRNAs were identified at 48 h feeding. The pathway analysis of the predicted target genes revealed that the plant hormone signal transduction, RNA transport, protein processing in the endoplasmic reticulum, zeatin biosynthesis, ubiquinone and other terpenoid-quinone biosynthesis, and isoquinoline alkaloid biosynthesis may play important roles in soybean’s defense against the stress caused by bean pyralid larvae. According to conjoint analysis of the miRNA/mRNA, a total of 20 differentially expressed miRNAs were negatively correlated with 26 differentially expressed target genes. The qRT-PCR analysis verified that the small RNA sequencing results were credible. According to the analyses of the differentially expressed miRNAs, we speculated that miRNAs are more likely to play key roles in the resistance to insects. Gma-miR156q, Gma-miR166u, Gma-miR166b, Gma-miR166j-3p, Gma-miR319d, Gma-miR394a-3p, Gma-miR396e, and so on—as well as their negatively regulated differentially expressed target genes—may be involved in the regulation of soybean resistance to bean pyralid larvae. These results laid a foundation for further in-depth research regarding the action mechanisms of insect resistance.

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Genetic components and major QTL confer resistance to bean pyralid (Lamprosema indicata Fabricius) under multiple environments in four RIL populations of soybean

et al. 2012

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Bean pyralid (BP; Lamprosema indicata Fabricius) is one of the major leaf-feeding insects that affect soybean crops in central and southern China. Four recombinant inbred line populations (KY, WT, XG and SX) were tested during 2004-2006 in Nanjing, China, to identify quantitative trait loci (QTL) for resistance to BP on the basis of data for rolled leaflet percentage under field infestation conditions. The mapping was performed using QTL Network V2.0 and checked with Windows QTL Cartographer V2.5 and IciMapping V2.2. The results showed that 81-92 % of the phenotypic variation was accounted for by additive QTL (27-43 %), epistatic QTL pairs (5-13 %), and collective unmapped minor QTL (38-58 %). In total, 17 QTL were detected on 11 linkage groups, of which two had additive effects, six had both additive and epistatic effects, and nine had only epistatic effects. Eight epistatic QTL pairs were observed, of which three pairs involved two QTL with additive effects, one involved one QTL with additive effect, and four involved no QTL with additive effects. Different genetic structures for BP resistance were found among the populations. Eight QTL (five additive and three epistatic pairs) were detected in KY, ten QTL (four additive and five epistatic pairs) were detected in WT, and only one additive QTL was detected in both the XG and the SX populations. BP12-1 and BP1-1 are major QTL, with the former accounting for 15, 31, and 50 % of the total genetic variation (including epistasis) in KY, WT, and XG, respectively, and the latter accounting for 13 and 32 % of the total genetic variation in KY and SX, respectively. The additive × year and epistasis × year interaction effects were negligible, indicating that the QTL were stable over the years. Because 41-68 % of the total genetic variation could not be accounted for by these QTL, the use of both identified QTL and unmapped minor QTL in breeding for BP resistance should be considered.

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Development under Constant Temperatures and Seasonal Prevalence in Soybean Field of the Bean Pyralid, Omiodes indicatus (Lepidoptera: Crambidae)

Cited by 5 publications

References 1 publication

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

Determination of the MiRNAs Related to Bean Pyralid Larvae Resistance in Soybean Using Small RNA and Transcriptome Sequencing

Genetic components and major QTL confer resistance to bean pyralid (Lamprosema indicata Fabricius) under multiple environments in four RIL populations of soybean

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