Development, validation and clinical evaluation of a dried urine spot method for determination of hippuric acid and creatinine

Antunes, Marina Venzon; Niederauer, Camila Ghani; Linden, Rafael

doi:10.1016/j.clinbiochem.2013.07.004

Cited by 27 publications

(23 citation statements)

References 16 publications

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“…We successfully detected all ten of the target metabolites, in contrast with the detection of only one metabolite in a previous study [17]; the enhanced analytical sensitivity of the developed protocol is attributed to the combination of EME with the sensitive LC-MS/MS analysis. EME was optimized by using design of experiments, a statistical method, and reasonable recoveries were obtained.…”

Section: Resultscontrasting

confidence: 64%

“…In spite of the relatively small sample size, the sensitivity of the developed method is comparable to or better than that of existing methods. A significant improvement in the LOQ compared to the only reported DUS analysis [17] highlights the potential of this method to be a promising approach in this research field.…”

Section: Comparison With Other Methodsmentioning

confidence: 81%

“…A comparison of the developed method with other reported methods is shown in Table 6 [17,[35][36][37]. The data indicate that the proposed technique is efficient and sensitive for extraction of the model analytes.…”

Section: Comparison With Other Methodsmentioning

confidence: 94%

“…However, it is difficult to achieve sufficient sensitivity with the use of DUS due to the small sample size. Because of this limitation, hippuric acid, which is in large supply in the human body, was the only compound successfully determined in the analysis of VOC metabolites in DUS [17]. In the case of dried blood spots, a few techniques have been developed and applied to enhance the sensitivity of the analysis [18][19][20][21], but no trials have been performed using DUS.…”

Section: Introductionmentioning

confidence: 99%

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Highly sensitive electromembrane extraction for the determination of volatile organic compound metabolites in dried urine spot

Suh

Eom

Kim

et al. 2015

Journal of Chromatography A

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Section: Resultscontrasting

confidence: 64%

Section: Comparison With Other Methodsmentioning

confidence: 81%

Section: Comparison With Other Methodsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Highly sensitive electromembrane extraction for the determination of volatile organic compound metabolites in dried urine spot

Suh

Eom

Kim

et al. 2015

Journal of Chromatography A

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“…5,[15][16] Esse conservador está presente em refrigerantes, sucos de frutas, margarina, molho de tomate, enlatados. [17][18][19] Um estudo identificou os refrigerantes como a principal fonte de benzoato no Brasil, representando mais de 80% da ingestão diária do conservador.…”

Section: Resultsunclassified

Análise simultânea dos indicadores biológicos de exposição aos solventes etilbenzeno, estireno, tolueno e xileno na urina por CLAE-UV

Yamamoto¹,

Moraes²,

Silva³

et al. 2016

Quim. Nova

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SIMULTANEOUS ANALYSIS OF BIOLOGICAL INDICATORS OF EXPOSURE TO ETHYLBENZENE, STYRENE, TOLUENE AND XYLENE SOLVENTS IN URINE BY HPLC-UV.A method for simultaneous analysis of phenylglyoxylic (PGA), mandelic (MA), hippuric (HA), orto-, meta-and para-methylhippuric (o-, m-and p-MHA) acids in urine, by high performance liquid chromatography with UV detection was developed and validated. The substances are biotransformation products used as biological indicators to assess the exposure of workers, respectively, to ethylbenzene, styrene, toluene and xylene. The urinary metabolites were separated using a C18 column and a mixture of 5 mM phosphate buffer pH 2.6:acetonitrile (92:8, v/v) as mobile phase. Urine samples were extracted with dichloromethane:ethyl acetate (70:30, v/v). The method used phenacetin as internal standard and was linear in the interval of 100-500 µg mL -1 for PGA and MA and 150-700 µg mL -1 for HA and MHA. The detection limits in µg mL -1, were 19.8 for PGA, 1.7 for MA, 4.1 for HA, 3.9 for o-MHA, 3.3 for m-MHA and 7.3 for p-MHA. Intra-and inter-assay precisions (as relative standard deviation) were all less than 15% and accuracy (as relative standard error) did not exceed 12.3%. The recovery was higher than 65% for all metabolites. The developed method will be applied to biological evaluation of workers exposure to these solvents.Keywords: volatile organic compounds; urine; biological monitoring; HPLC-UV. INTRODUÇÃOOs compostos orgânicos etilbenzeno, estireno, tolueno e xileno são amplamente utilizados como solventes em indústrias de plástico, resina, borracha e farmacêutica.1-4 Também são constituintes de combustíveis, tintas, vernizes e colas. [4][5][6] Trabalhadores expostos a níveis elevados desses solventes podem apresentar efeitos adversos, como irritação (da pele e do trato respiratório), alterações do sistema nervoso central, alterações funcionais nos nervos ótico e óptico. 5 Portanto, a monitorização da exposição, tanto ambiental quanto biológica, são atividades complementares e fundamentais na prevenção de tais efeitos. A monitorização ambiental avalia o risco à saúde do indivíduo exposto através da concentração dos solventes na zona respiratória, comparando-a aos Limites de Exposição Ocupacional. Por sua vez, a monitorização biológica pode avaliar a dose interna do composto para verificar se a exposição do trabalhador está em níveis aceitáveis, por comparação com o respectivo Limite para o Indicador Biológico utilizado. Para os solventes, os marcadores biológicos (ou indicadores biológicos) podem ser a própria substância ou os seus metabólitos presentes em matrizes biológicas. 7A principal via de absorção do etilbenzeno, estireno, tolueno e xileno é a via respiratória e a eliminação ocorre, principalmente, através dos metabólitos urinários.5 Os principais metabólitos do etilbenzeno e do estireno são o ácido mandélico (AM) e o ácido fenilglioxílico (AFG). No entanto, esses metabólitos não são indicadores específicos, pois também são formados quando há exposição a outras substâncias, por exempl...

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LC–MS‐Based Simultaneous Determination of Biomarkers in Dried Urine Spots for the Detection of Cofactor‐Dependent Metabolic Disorders in Neonates

Raveendran,

Gupta,

Lewis

et al. 2024

J of Separation Science

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Deficiency of cofactors for various enzymes can lead to inborn errors of metabolism. These conditions frequently occur as seizures, which lead to permanent brain damage. Newborn screening for biomarkers associated with these disorders can help in early detection and treatment. Our objective was to establish a liquid chromatography mass spectrometry technique for quantifying biomarkers in dried urine spots to detect specific vitamin‐responsive inborn errors metabolism. Biomarkers were extracted from dried urine spots using a methanol:0.1% v/v formic acid solution (75:25) containing an internal standard mixture. Separation was achieved using a Luna PFP column (150 mm × 4.6 mm, 3 µm) under gradient elution conditions. The LC–MS technique was validated as per ICH M10 guidelines. Urine samples from healthy newborns in Udupi district, South India, were analyzed to establish reference values for these biomarkers. The method demonstrated excellent linearity (R2 > 0.99) with low limits of quantification: 0.1 µg/mL for leucine, isoleucine, valine, proline, hydroxyproline, methylmalonic acid, and 3‐hydroxyisovaleric acid; 0.01 µg/mL for pipecolic acid and α‐aminoadipic semialdehyde; and 0.03 µg/mL for piperideine‐6‐carboxylate. Interconvertibility between urine and dried urine spot assays was observed from the results of the regression and Bland–Altman analyses. Reference intervals for these biomarkers in the Udupi neonatal population were established using the validated dried urine spot method.

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Development, validation and clinical evaluation of a dried urine spot method for determination of hippuric acid and creatinine

Cited by 27 publications

References 16 publications

Highly sensitive electromembrane extraction for the determination of volatile organic compound metabolites in dried urine spot

Highly sensitive electromembrane extraction for the determination of volatile organic compound metabolites in dried urine spot

Análise simultânea dos indicadores biológicos de exposição aos solventes etilbenzeno, estireno, tolueno e xileno na urina por CLAE-UV

LC–MS‐Based Simultaneous Determination of Biomarkers in Dried Urine Spots for the Detection of Cofactor‐Dependent Metabolic Disorders in Neonates

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