Development, verification, and validation of an RT-qPCR-based protocol for Yellow Fever diagnosis

Abstract

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A cost-effective RNA extraction and RT-qPCR approach to detect California serogroup viruses from pooled mosquito samples

“…There appears to be limitations in accessible, user-friendly internal controls tailored for MBD screening via RT-qPCR. Many approaches rely on spiking-based internal controls 15 , 16 which, while effective, increases complexity and elevated costs to high-throughput viral screening workflows. Our objective was to devise a dependable internal control that could integrate with multiplexed RT-qPCR assays through the straightforward addition of primers and a probe.…”

Validation of the performance of a point of care molecular test for leprosy: from a simplified DNA extraction protocol to a portable qPCR

“…Molecular tests, particularly real-time polymerase chain reaction (qPCR), are sensitive and specific, contributing to the early identification of various pathologies (MADAMET et al, 2022; RAMPAZZO et al, 2022; YU et al, 2021). Although molecular detection tests are available, the diagnosis of leprosy continues to rely primarily on clinical diagnosis (WHO, 2022).…”