Developmental analysis of elongation factor-1 alpha expression in transgenic tobacco.

Ursin, Virginia M.; Irvine, Jonathan M.; Hiatt, William R.; Shewmaker, Christine K.

doi:10.1105/tpc.3.6.583

Cited by 40 publications

(20 citation statements)

References 19 publications

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“…The activity of a partially puri®ed EF-2 from wheat germ has been examined (Smailov et al 1993). However, another family of elongation factors of the 1-a type, homologous to the bacterial factor EF-Tu, has been implicated in the control of plant development through the regulation of protein synthesis (Ursin et al 1991). EF-1a is thought to aect plant growth and dierentiation by interacting with microtubules (Durso and Cyr 1994).…”

Section: Discussionmentioning

confidence: 99%

Isolation of a set of ripening-related genes from strawberry: their identification and possible relationship to fruit quality traits

Manning¹

1998

Planta

165

101

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The ripening of strawberry (Fragaria ananassa Duch.), a non-climacteric fruit, is a complex developmental process that involves many changes in gene expression. To understand how these changes relate to the biochemistry and composition of the fruit the specific genes involved have been examined. A high-quality cDNA library prepared from ripe strawberry fruit was differentially screened for ripening-related clones using cDNA from ripe and white fruits. From 112 up-regulated clones obtained in the primary screen, 66 differentially expressed clones were isolated from the secondary screen. The partial sequences of these cDNAs were compared with database sequences and 26 families of non-redundant clones were identified. Northern analysis confirmed that all of these cDNAs were ripening-enhanced. The expression of many of their corresponding genes was negatively regulated in auxin-treated fruit. These sequences, several of which are novel to fruits, encode proteins involved in key metabolic events including anthocyanin biosynthesis, cell wall degradation, sucrose and lipid metabolism, protein synthesis and degradation, and respiration. These findings are discussed in relation to the role of these genes in determining fruit quality characteristics.

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Section: Discussionmentioning

confidence: 99%

Isolation of a set of ripening-related genes from strawberry: their identification and possible relationship to fruit quality traits

Manning¹

1998

Planta

165

101

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“…A previous study in tobacco plants showed that its expression was higher in younger, developing tissues than in older or more mature tissues [41]. Moreover, the same gene was also highlighted as a candidate gene for proteomic analysis of shoot apical meristem transition from dormancy to activation in Cunninghamia lanceolata (Lamb.)…”

Section: A High Input Of Energy Is Required During Bud Sprouting In Cmentioning

confidence: 96%

Comprehensive Analysis of the Cork Oak (Quercus suber) Transcriptome Involved in the Regulation of Bud Sprouting

Usié

Simões

Barbosa

et al. 2017

Forests

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Cork oaks show a high capacity of bud sprouting as a response to injury, which is important for species survival when dealing with external factors, such as drought or fires. The characterization of the cork oak transcriptome involved in the different stages of bud sprouting is essential to understanding the mechanisms involved in these processes. In this study, the transcriptional profile of different stages of bud sprouting, namely (1) dormant bud and (2) bud swollen, vs. (3) red bud and (4) open bud, was analyzed in trees growing under natural conditions. The transcriptome analysis indicated the involvement of genes related with energy production (linking the TCA (tricarboxylic acid) cycle and the electron transport system), hormonal regulation, water status, and synthesis of polysaccharides. These results pinpoint the different mechanisms involved in the early and later stages of bud sprouting. Furthermore, some genes, which are involved in bud development and conserved between species, were also identified at the transcriptional level. This study provides the first set of results that will be useful for the discovery of genes related with the mechanisms regulating bud sprouting in cork oak.

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“…In tomato (Pokalsky et al, 1989), tobacco (Ursin et al, 1991;Marty et al, 1993), and possibly Arabidopsis (Curie et al, 1993), elevated levels of EF-lu gene expression were indicated in developing tissues. Auxin (Ursin et al, 1991), low temperature (Dunn et al, 1993), and light (Aguilar et al, 1991) appear to modulate expression of some EF-lu genes, and multigene families are present in severa1 plants (Axelos et al, 1989;Pokalsky et al, 1989;Aguilar et al, 1991;Dunn et al, 1993). A gene family has been found in carrot cells as well (Kawahara et al, 1992), and Figure 46 presents data consistent with this finding.…”

mentioning

confidence: 99%

A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.

1994

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The microtubules (MTs) of higher plant cells a= organized into arrays with essential functions in plant cell growth and differentiation; however, molecular mechanisms underlying the organization and regulation of these arrays remain largely unknown. We have approached this problem using tubulin affinity chromatography to isolate carrot proteins that interact with MTs. From these proteins, a 50-kD polypeptide was selectively purified by exploiting its Ca2+-dependent binding to calmodulin (CaM). This polypeptide was identified as a homolog of elongation factor-lu (EF-1a)-a highly conserved and ubiqultous protein translation factor. The carrot EF-la homolog bundles MTs in vitro, and moreover, this bundling is modulated by the addition of Ca2+ and CaM together (Ca2+/CaM). A direct binding between the EF-la homolog and MTs was demonstrated, providing nove1 evidence for such an interaction. Based on these findings, and others discussed herein, we propose that an EF-la homolog mediates the lateral association of MTs in plant celk by a Ca2+/CaM-sensitive mechanism. INTRODUCTIONMicrotubules (MTs) participate in a number of essential processes in eukaryotes (MacRae, 1992b). In cells of higher plants, MTs are generally arranged into four structurally and functionally distinct, cell cycle-dependent MT arrays (Goddard et al., 1994;Lambert and Lloyd, 1994). During interphase, the MT array in a cell's cortex is involved in the extracellular deposition of cellulose microfibrils (Giddings and Staehelin, 1991). During G2 of the cell cycle, this array is succeeded by a narrower preprophase band involved in establishing the site of the incipient division plane (Palevitz, 1991; Wick, 1991). The preprophase band resolves into the mitotic spindle apparatus as M phase progresses Palevitz, 1993). The fourth MT array, the phragmoplast, appears as cytokinesis begins and is involved in depositing the new cell plate at the position previously marked by the preprophase band (Lloyd, The bases by which plant MTs are organized into higher order arrays and by which those arrays are regulated remain largely unknown (Lambert, 1993). MTs are hollow cylinders with 25-nm diameters, and tubulin is the principal protein subunit of MTs. MTs assembled from pure tubulin have a limited range of behavior-they assemble or disassemble. This limited range of behavior can only partly account for the diverse structures and functions of MT arrays.Nearly all species have multiple tubulin genes encoding various tubulin isotypes (for plant genes, see Goddard et al., 1994), and it has been proposed that the isotypes may provide a 1991).To whom correspondence should be addressed. means for serving different MT functions (Fulton and Simpson, 1976). However, nearly all existing evidence indicates that tubulin isotypes are functionally interchangeable (LudueAa, 1993). In general, tubulins are COnseNed even between kingdoms (Fosket and Morejohn, 1992; Burns and Surridge, 1994). In fact, animal brain tubulin that is microinjected into living plant cells incorporates into t...

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Developmental analysis of elongation factor-1 alpha expression in transgenic tobacco.

Cited by 40 publications

References 19 publications

Isolation of a set of ripening-related genes from strawberry: their identification and possible relationship to fruit quality traits

Isolation of a set of ripening-related genes from strawberry: their identification and possible relationship to fruit quality traits

Comprehensive Analysis of the Cork Oak (Quercus suber) Transcriptome Involved in the Regulation of Bud Sprouting

A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.

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