Developmental and Biochemical Genetics of Lactate Dehydrogenase Isozymes in Fishes

Whitt, Gregory S.; Miller, E. T.; Shaklee, James B.

doi:10.1007/978-3-642-65700-9_23

Cited by 29 publications

(3 citation statements)

References 65 publications

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“…Hi fossilis exhibits a 5 isozyme pattern and C. batrachus exhibits a 4 isozyme pattern. Finding of such variable number of isozymes is not unusual among fishes (Cowan 1972;Whitt et al 1973;Champion and Whitt 1976). The 5 isozyme fish makes all the 5 tetramers, the 4 isozyme fish makes all the isozymes except for A 3B i-The presence of only 4 isozymes is most likely the result of restricted subunit assembly (Whitt 1970a) or an instability of the asymmetrical heteropolymer A 3B 1 .…”

Section: Discussionmentioning

confidence: 99%

Tissue distribution and characterization of LDH isozymes in two fishes of the order cypriniformes

Triveni

Rao

1986

Proc. Indian Acad. Sci. (Anim. Sci.)

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Electrophoretic analysis of the lactate dehydrogenase isozyme patterns of several tissues of H eteropneustes[ossilis and Clarias batrachus indicated that in addition to the LO H A and B genes found in these species the liver tissue of Clarias batrachus also possesses a liver specific LOH C. gene.The relative mobilities of the LOH-B 4 and -A 4 isozymes in Heteropneustes fossilis and Clarias batrachus, are reversed. Further there are significant quantitative differences in the amounts of specific isozymes in homologous tissues of these two species and also in different tissues of a single species as documented in densitomeric tracings. The subunit composition of the LOH isozymes were confirmed by immunological procedures.

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Section: Discussionmentioning

confidence: 99%

Tissue distribution and characterization of LDH isozymes in two fishes of the order cypriniformes

Triveni

Rao

1986

Proc. Indian Acad. Sci. (Anim. Sci.)

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“…As the small size of tetras requires the use of a magnification lens to collect separate organs, imposing a longer time to process the samples, 48 individuals of each species were transversely divided into two portions: head (brain, heart, and liver) and tail (mostly white muscle). Each portion represents the differential contribution of the two Ldh genes: the head portion is expected to have a dominance of the ldh-b -like isoform, and the tail portion a dominance of the ldh-a -like isoform, as previously described for many other teleost species ( Whitt et al , 1973 ; Almeida-Val and Val, 1993 ). Both body portions (or sides) were manually homogenized in an ice-cold buffer solution containing 150 mM imidazole, 1 mM EDTA and 1% Triton X-100 (pH 7.4) in a tissue/buffer ratio 1:10 (w/v).…”

Section: Methodsmentioning

confidence: 87%

Differential survivorship of congeneric ornamental fishes under forecasted climate changes are related to anaerobic potential

et al. 2018

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Two Amazonian closely related tetras – cardinal Paracheirodon axelrodi and green neon P. simulans – were artificially acclimatized to environmental chambers mimicking future climate change scenarios (mild, moderate and extreme), using a microcosm facility. P. simulans survived (100%) to all scenarios after 30 days exposure, while P. axelrodi presented decreasing survival percentages according to environmental severity. These differences may be the reflection of distinct natural acclimatization to microhabitats between the species, which differ in thermal conditions. Survival responses might be related to differences in relative gene expression of lactate dehydrogenase (Ldh), suggesting that P. axelrodi anaerobic potential is lower or non-existent compared to P. simulans, not tolerating long-term thermal challenges. Accordingly, increases in temperature and in CO2 levels caused increases in energy demand and resulted in activation of the anaerobic pathway, as demonstrated by the higher enzyme levels measured in head and tail portions of both species. Sustained anaerobic glycolysis is possible when fish live in challenging environments (low oxygen or high temperature). Our results clearly show that P. simulans has a larger scope for survival to higher energy demands due to its increased anaerobic potential compared to P. axelrodi.

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“…Earlier work on biochemical and immunological characterizations had established close similarity of LDH-C with LDH-B. It was suggested that a -B gene duplication event gave rise to LDH-C at about the time of origin of the bony fishes, approximately 350 million years ago (Whitt 1969;Whitt et al 1973;Markert et al 1975). An alternative view that proposes closeness between LDH-A with -C genes has been put forth by Li & Tsoi (2002).…”

Section: Incubation Time (In Min)mentioning

confidence: 99%

Functional and adaptive significance of differentially expressed lactate dehydrogenase isoenzymes in tissues of four obligatory air-breathing Channa species

Ahmad

2009

Biologia

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This study investigates the differential expression of lactate dehydrogenase (LDH) isoenzymes in the genus Channa using PAGE. With the help of obligate air-breathing, all of the selected species can sustain water deprivation to varying degrees. In subunit composition and higher electrophoretic mobility of LDH-A4, the profiles of channid species were similar to other teleosts documented in the literature. However, inter-and intra-species differences, with particular reference to aerobic/anaerobic metabolic options, existed. Whereas glycolysis in Channa punctata appears to depend largely on aerobic LDH-B and partly on anaerobic LDH-A, metabolism in C. gachua, C. striata and C. marulius depends exclusively on the activity of anaerobic LDH-A. Expression of the third locus Ldh-C was recorded in the eyes of C. marulius, in addition to C. gachua. Heat inactivation experiments reveal species differences between LDH isoenzymes and a general order of the relative stabilities: LDH-C > LDH-B > LDH-A. Metabolic and evolutionary implications of the findings have also been discussed.

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Developmental and Biochemical Genetics of Lactate Dehydrogenase Isozymes in Fishes

Cited by 29 publications

References 65 publications

Tissue distribution and characterization of LDH isozymes in two fishes of the order cypriniformes

Tissue distribution and characterization of LDH isozymes in two fishes of the order cypriniformes

Differential survivorship of congeneric ornamental fishes under forecasted climate changes are related to anaerobic potential

Functional and adaptive significance of differentially expressed lactate dehydrogenase isoenzymes in tissues of four obligatory air-breathing Channa species

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