Developmental and insecticide-resistant insights from the de novo assembled transcriptome of the diamondback moth, Plutella xylostella

He, Weiyi; You, Minsheng; Vasseur, Liette; Guang, Yang; Xie, Ming; Cui, Kai; Bai, Jing; Liu, Chunhui; Li, Xiaojing; Xu, Xiufeng; Huang, Shiguo

doi:10.1016/j.ygeno.2011.12.009

Cited by 77 publications

(75 citation statements)

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“…6 and Supplementary Table 6). The assembly covered 85.5% of a set of protein-coding ESTs (Supplementary Tables 7 and 8) generated by transcriptome sequencing 11 . Alignment of a subject scaffold against a 126-kb BAC (GenBank GU058050) from an alternative strain (Geneva 88) showed extensive structural variations between haplotypes.…”

Section: (Supplementarymentioning

confidence: 99%

“…On the basis of P. xylostella transcriptome data 11 , we identified 354 preferentially expressed genes in larvae ( Supplementary Fig. 15), and a set of these genes is involved in sulfate metabolism, some of which were validated using quantitative RT-PCR for gene expression analysis ( Supplementary Figs.…”

Section: (Supplementarymentioning

confidence: 99%

“…5a). Larval transcriptomes were sequenced from the Fuzhou-S strain that was genotyped and from two substrains selected for resistance to chlorpyrifos or fipronil 11 . ABC transporter genes were upregulated more frequently than GSTs, COEs or P450s in insecticide-resistant larvae (Supplementary Fig.…”

Section: (Supplementarymentioning

confidence: 99%

“…On the basis of the DBM genome and the transcriptomes for newly laid eggs, third-instar larvae, pupae and virgin adults, we analyzed differential gene expressions in four developmental stages using the same statistical approach 11 . The larval preferentially expressed genes were defined as genes that were highly expressed in the larval stage compared to the other three developmental stages, with RPKM ratio ≥ 8 fold (upregulated) and false discovery rate (FDR) ≤ 0.001.…”

Section: Digital Gene Expression (Dge)mentioning

confidence: 99%

“…Transcriptome reads 11 were mapped onto the genome using TopHat 46 . We then used Cufflinks 47 (with default parameters) to assemble transcripts and integrated the transcripts with the GLEAN set by filtering out redundancy and the genes with ≥10% uncertain bases and coding region lengths of ≤150 bp.…”

Section: Digital Gene Expression (Dge)mentioning

confidence: 99%

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A heterozygous moth genome provides insights into herbivory and detoxification

et al. 2013

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l e t t e r sHow an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants 1 , but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood 2 . We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management.The global pest P. xylostella (Lepidoptera: Yponomeutidae) is thought to have coevolved with the crucifer plant family 3 ( Supplementary Fig. 1) and has become the most destructive pest of economically important food crops, including rapeseed, cauliflower and cabbage 4 . Recently, the total cost of damage and management worldwide was estimated at $4-5 billion per annum 5,6 . This insect is the first species to have evolved resistance to dichlorodiphenyltrichloroethane (DDT) in the 1950s 7 and to Bacillus thuringiensis (Bt) toxins in the 1990s 8 and has developed resistance to all classes of insecticide, making it increasingly difficult to control 9,10 . P. xylostella provides an exceptional system for understanding the genetic and molecular bases of how insect herbivores cope with the broad range of plant defenses and chemicals encountered in the environment (Supplementary Fig. 2).We used a P. xylostella strain (Fuzhou-S) collected from a field in Fuzhou in southeastern China (26.08 °N, 119.28 °E) for sequencing ( Supplementary Fig. 1). Whole-genome shotgun-based Illumina sequencing of single individuals (Supplementary Table 1), even after ten generations of laboratory inbreeding, resulted in a poor initial assembly (N50 = 2.4 kb, representing the size above which 50% of the total length of the sequences is included), owing to high levels of heterozygosity ( Supplementary Figs. 3 and 4 and Supplementary Table 2). Subsequently, we sequenced 100,800 fosmid clones (comprising ~10× the genome length) to a depth of 200× ( Supplementary Fig. 5 and Supplementary Tables 3-5), assembling the resulting sequence data into 1,819 scaffolds, with an N50 of 737 kb, spanning ~394 Mb of the genome sequence (version 1; Supplementary Fig. 6 and Supplementary Table 6). The assembly covered 85.5% of a set of protein-coding ESTs (Supplementary Tables 7 and 8) generated by transcriptome sequencing 11 . Alignment of a subject scaffold against a 126-kb BAC (GenBank GU058050) from an altern...

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Section: (Supplementarymentioning

confidence: 99%

Section: (Supplementarymentioning

confidence: 99%

Section: (Supplementarymentioning

confidence: 99%

Section: Digital Gene Expression (Dge)mentioning

confidence: 99%

Section: Digital Gene Expression (Dge)mentioning

confidence: 99%

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A heterozygous moth genome provides insights into herbivory and detoxification

et al. 2013

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Comparative transcriptome analysis provides insights of anti‐insect molecular mechanism of Cassia obtusifolia trypsin inhibitor against Pieris rapae

Xiang

Zhang

Yin

et al. 2017

Arch Insect Biochem Physiol

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Pieris rapae, a serious Lepidoptera pest of cultivated crucifers, utilizes midgut enzymes to digest food and detoxify secondary metabolites from host plants. A recombinant trypsin inhibitor (COTI)1 from nonhost plant, Cassia obtusifolia, significantly decreased activities of trypsin-like proteases in the larval midgut on Pieris rapae and could suppress the growth of larvae. In order to know how COTI took effect, transcriptional profiles of P. rapae midgut in response to COTI was studied. A total of 51,544 unigenes were generated and 45.86% of which had homologs in public databases. Most of the regulated genes associated with digestion, detoxification, homeostasis, and resistance were downregulated after ingestion of COTI. Meanwhile, several unigenes in the integrin signaling pathway might be involved in response to COTI. Furthermore, using comparative transcriptome analysis, we detected differently expressing genes and identified a new reference gene, UPF3, by qRT-polymerase chain reaction (PCR). Therefore, it was suggested that not only proteolysis inhibition, but also suppression of expression of genes involved in metabolism, development, signaling, and defense might account for the anti-insect resistance of COTI. K E Y W O R D SCassia obtusifolia, nonhost plant, physiological response, Pieris rapae, trypsin inhibitor

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Molecular cloning, expression profiling, and functional analysis of a broad‐complex isoform 2/3 (Br‐Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.)

Huang

Fang

Wang

et al. 2019

Arch Insect Biochem Physiol

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The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a widespread and destructive pest of cruciferous crops. New strategies for controlling it are needed because it is rapidly developing resistance to conventional pesticides. In insects, transcription factors (TFs) including broad-complex (Br-C) are thought to be useful for insecticide development because they are able to regulate the transcription of functional genes involved in responses to external stimuli including insecticides. In the present study, we cloned and sequenced the open reading frames (ORFs) of three BTB-ZF encoding genes from the diamondback moth deposited in the National Center for Biotechnology Information (NCBI) database under accessions MG753773, MG288674, and MG753772. The lengths of these ORFs were 1,680, 1,428, and 1,647 bp, respectively. The phylogenetic analysis based on the predicted amino acid sequences of ZF domains showed that MG753773 and MG288674 belonged to Z2/Z3 and Z7 of Br-C while MG753772 belonged to Ttk types. In the agreement, the highest expression level of MG753773 occurred during the Arch. Insect Biochem. Physiol. 2019;101:e21549.wileyonlinelibrary.com/journal/arch

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Developmental and insecticide-resistant insights from the de novo assembled transcriptome of the diamondback moth, Plutella xylostella

Cited by 77 publications

References 46 publications

A heterozygous moth genome provides insights into herbivory and detoxification

A heterozygous moth genome provides insights into herbivory and detoxification

Comparative transcriptome analysis provides insights of anti‐insect molecular mechanism of Cassia obtusifolia trypsin inhibitor against Pieris rapae

Molecular cloning, expression profiling, and functional analysis of a broad‐complex isoform 2/3 (Br‐Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.)

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