Developmental and stress regulation of gene expression for a 9-cis-epoxycarotenoid dioxygenase, CstNCED, isolated from Crocus sativus stigmas

Ahrazem, Oussama; Rubio-Moraga, Ángela; Trapero, Almudena; Gómez-Gómez, Lourdes

doi:10.1093/jxb/err293

Cited by 38 publications

(25 citation statements)

References 75 publications

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“…The first reaction, the conversion of zeaxanthin to all-trans-violaxanthin is catalysed by zeaxanthin epoxidase (ZEP) (Seo and Koshiba, 2002) that is encoded by ABA1 in Arabidopsis (Xiong et al, 2002) and SlZEP1 gene in tomato (Thompson et al, 2000). The rate-limiting step of ABA biosynthesis is catalysed by 9-cis-epoxycarotenoid dioxygenase (SlNCED), which produces a C-15 intermedier, xanthoxin from 9-cis-isomers of violaxanthin and neoxanthin (Thompson et al, 2000;Ahrazem et al, 2012). Xanthoxin is converted into abscisic aldehyde (ABAld) by a short chain dehydrogenase/reductase (Gonzáles-Guzman et al, 2002) and the oxidation of ABAld to ABA is catalysed by aldehyde oxydases (AOs) (Schwartz and Zeevaart, 2010).…”

Section: Introductionmentioning

confidence: 99%

Hardening with salicylic acid induces concentration-dependent changes in abscisic acid biosynthesis of tomato under salt stress

Horváth

Csiszár

Gallé

et al. 2015

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 99%

Hardening with salicylic acid induces concentration-dependent changes in abscisic acid biosynthesis of tomato under salt stress

Horváth

Csiszár

Gallé

et al. 2015

Journal of Plant Physiology

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“…This last behavior is also followed by ELIPb . CsNCED , a gene encoding for a key enzyme involved in ABA biosynthesis in saffron [42], showed the highest levels of expression in stigmas at postanthesis, suggesting a correlation of ELIPa , b and c expression with stigma senescence, controlled in Crocus flowers by ABA. In fact, ELIPa and c expression levels increased by different abiotic stresses and ELIPc transcripts were clearly induced by ABA.…”

Section: Discussionmentioning

confidence: 99%

Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach

Ahrazem

Argandoña

Castillo³

et al. 2016

PLoS ONE

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Using a subtractive hybridization approach, differentially expressed genes involved in the light response in saffron stigmas were identified. Twenty-two differentially expressed transcript-derived fragments were cloned and sequenced. Two of them were highly induced by light and had sequence similarity to early inducible proteins (ELIP) and SOUL heme-binding proteins. Using these sequences, we searched for other family members expressed in saffron stigma. ELIP and SOUL are represented by small gene families in saffron, with four and five members, respectively. The expression of these genes was analyzed during the development of the stigma and in light and dark conditions. ELIP transcripts were detected in all the developmental stages showing much higher expression levels in the developed stigmas of saffron and all were up-regulated by light but at different levels. By contrast, only one SOUL gene was up-regulated by light and was highly expressed in the stigma at anthesis. Both the ELIP and SOUL genes induced by light in saffron stigmas might be associated with the structural changes affecting the chromoplast of the stigma, as a result of light exposure, which promotes the development and increases the number of plastoglobules, specialized in the recruitment of specific proteins, which enables them to act in metabolite synthesis and disposal under changing environmental conditions and developmental stages.

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“…However, only one NCED gene, CsNCED, has been studied in detail. 46 CsNCED is expressed in floral and corm tissues. In stigmas, CsNCED transcript abundance closely mirrors ABA content with a maxima a day after anthesis, and it has been suggested that the ABA levels in the stigma could be related to the initiation of the senescent process in this tissue.…”

Section: ■ Introductionmentioning

confidence: 99%

“…47 During corm development, zeaxanthin levels are correlated with the increase in CsNCED expression, suggesting a coordinate regulation of the carotenoid and ABA biosynthetic pathways during corm dormancy. 46 Mature saffron corms usually show one to three apical dominant buds, which will sprout in the following season plus many axillary dormant buds. 22 Each axillary bud has the same developmental potential as the primary shoot apical meristem in its ability to produce a growing shoot axis ( Figure 4).…”

Section: ■ Introductionmentioning

confidence: 99%

Saffron: Its Phytochemistry, Developmental Processes, and Biotechnological Prospects

Ahrazem

Rubio-Moraga

Nebauer

et al. 2015

J. Agric. Food Chem.

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The present state of knowledge concerning developmental processes and the secondary metabolism of saffron, Crocus sativus L. (Iridaceae), along with the genes involved in these processes so far known, is reviewed. Flowers and corms constitute the most valuable parts of saffron. Corm and flower development are two key aspects to be studied in saffron to increase the yield and quality of the spice, to raise its reproductive rate, and to implement new production systems. Important knowledge about the physiology of flowering and vegetative growth has been acquired in recent years, but there is still only limited information on molecular mechanisms controlling these processes. Although some genes involved in flower formation and meristem transition in other species have been isolated in saffron, the role of these genes in this species awaits further progress. Also, genes related with the synthesis pathway of abscisic acid and strigolactones, growth regulators related with bud endodormancy and apical dominance (paradormancy), have been isolated. However, the in-depth understanding of these processes as well as of corm development is far from being achieved. By contrast, saffron phytochemicals have been widely studied. The different flower tissues and the corm have been proved to be an important source of phytochemicals with pharmacological properties. The biotechnological prospects for saffron are here reviewed on the basis of the discovery of the enzymes involved in key aspects of saffron secondary metabolism, and we also analyze the possibility of transferring current knowledge about flowering and vegetative propagation in model species to the Crocus genus.

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Developmental and stress regulation of gene expression for a 9-cis-epoxycarotenoid dioxygenase, CstNCED, isolated from Crocus sativus stigmas

Cited by 38 publications

References 75 publications

Hardening with salicylic acid induces concentration-dependent changes in abscisic acid biosynthesis of tomato under salt stress

Hardening with salicylic acid induces concentration-dependent changes in abscisic acid biosynthesis of tomato under salt stress

Identification and Cloning of Differentially Expressed SOUL and ELIP Genes in Saffron Stigmas Using a Subtractive Hybridization Approach

Saffron: Its Phytochemistry, Developmental Processes, and Biotechnological Prospects

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