Developmental assessment of human vitrified-warmed blastocysts based on oxygen consumption

Yamanaka, M.; Hashimoto, Shu; Amo, A.; Ito-Sasaki, Takahiro; Abé, Hiroyuki; Morimoto, Yoshiharu

doi:10.1093/humrep/der324

Cited by 49 publications

(49 citation statements)

References 23 publications

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“…We included blastocysts whose degree of expansion was categorised as BL4/5, whose inner cell mass (ICM) was categorized before freezing as A or B, and whose trophectoderm (TE) grade was categorized before freezing as B or C, according to Gardner's criteria [8]. To obtain a numerical blastocyst morphology grading system based on Gardner's criteria, the blastocyst grade was converted to the multiplicative blastocyst quality score (BQS) [27,33]. The BQS is a measure of blastocyst quality based on established morphologic criteria, and is defined as the product of the degree of embryo development and ICM and TE grades, where grade A is given the value 3; grade B is 2; and grade C is 1.…”

Section: Study 2: Cell Membrane Damage After Vitrificationmentioning

confidence: 99%

A closed system supports the developmental competence of human embryos after vitrification

Hashimoto

Amo

Hama

et al. 2013

J Assist Reprod Genet

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Purpose Closed-system vitrification may enable the risk of contamination to be minimised. We performed three studies to compare the developmental competence of human embryos vitrified using either a closed vitrification system (CVS; Rapid-i®) or an open vitrification system (OVS; Cryo-top®). Methods The first study was performed in vitro using 66 zygotes previously vitrified at pronuclear stage. These were warmed and randomised 1:1 to revitrification using either the OVS or the CVS. After re-warming, embryo development and blastocyst cell number were assessed. For the second study, also performed in vitro, 60 vitrified-warmed blastocysts were randomised 1:1:1 into three groups (OVS or CVS revitrification, or no revitrification). The proportion of dead cells was assessed by staining. The third study was performed in vivo, using 263 high-grade blastocysts randomly assigned to vitrification using either the CVS (n=100) or the OVS (n=163). After warming, single blastocyst transfer was performed. Results There were no differences between the CVS and the OVS in survival rate (100 % vs. 97 %), blastulation rate (96 h: 50 % vs. 50 %; 120 h: 68 % vs. 56 %), proportion of good blastocysts (96 h: 32 % vs. 22 %, 120 h: 47 % vs. 41 %), or mean number of cells (137 vs. 138). The proportion of dead cells in blastocysts re-vitrified by CVS (31 %) was similar to that for OVS (38 %) and non-revitrification (32 %). In vivo, the implantation rate for blastocysts vitrified using the CVS (54 %) was similar to that with the OVS (53 %).Conclusion Our studies consistently indicate that human embryos may be vitrified using a CVS without impairment of developmental competence.

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Section: Study 2: Cell Membrane Damage After Vitrificationmentioning

confidence: 99%

A closed system supports the developmental competence of human embryos after vitrification

Hashimoto

Amo

Hama

et al. 2013

J Assist Reprod Genet

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“…Differences in OC rates between fresh and vitrified-warmed blastocysts were also reported, with higher OC in the formers (64). In addition, in vitrified-warmed blastocysts it has been found that OC was restored earlier in hatching or hatched compared to arrested or degenerated blastocysts, suggesting that it could be possible to identify vitrified-warmed blastocysts with high developmental potential on the basis of their respiratory activity (64). In another retrospective study (65), 47,741 OC measurements were performed on 575 injected oocytes obtained in 56 egg donation cycles whit embryo transfer on day-3, with the aim to evaluate the correlation of OC with embryo development and clinical outcomes.…”

Section: Oxygen Consumptionmentioning

confidence: 86%

“…In human, OC was demonstrated to be a quality marker of oocyte competence being affected by maternal age and basal FSH concentration (62) or ovarian stimulation protocols (63). Differences in OC rates between fresh and vitrified-warmed blastocysts were also reported, with higher OC in the formers (64). In addition, in vitrified-warmed blastocysts it has been found that OC was restored earlier in hatching or hatched compared to arrested or degenerated blastocysts, suggesting that it could be possible to identify vitrified-warmed blastocysts with high developmental potential on the basis of their respiratory activity (64).…”

Section: Oxygen Consumptionmentioning

confidence: 99%

Unconventional embryo selection strategies: a look into the future

Maria¹

2016

CCE

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SummaryThe assessment of an unequivocal method able to identify the embryo with the higher possibility to implant, leading to a single live birth, is crucial for the success of an infertility treatment. Standard morphology evaluation, morphokinetic analysis and preimplantation genetic screening are the more employed embryo selection strategies in reproductive medicine centers. However, the results and the applicability of these methodologies are still controversial. Therefore, there is the need to find additional technologies to clinically select developing embryos. The challenge is to set-up a predictive, not invasive, reproducible, objective and effective method able to select the best embryo. In this review, we present several unconventional methodologies of embryo evaluation, analyzing their possible future application in human reproduction.

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“…Furthermore, the oxygen uptake by vitrified bovine blastocysts, 18 hours after thawing, was the same as that of the non‐vitrified blastocysts. Another study has found that, after 6 hours, the oxygen consumption rate of the vitrified and the heated human blastocysts in the hatched group had recovered to that exhibited by the fresh blastocysts 21, 22…”

Section: Effect Of Freezing and Thawing In Assisted Reproductive Techmentioning

confidence: 99%

Theory about the Embryo Cryo‐Treatment

Vladimirov

Tacheva

Dı́ez

2017

Reprod Medicine & Biology

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BackgroundTo create hypothesis, which can give a logical explanation related to the benefits of freezing/thawing embryos. Cryopreservation is not only a technology used for storing embryos, but also a method of embryo treatment that can potentially improve the success rate in infertile couples.MethodsFrom the analysis of multiple results in assisted reproductive technology, which have no satisfactory explanation to date, we found evidence to support a ‘therapeutic’ effect of the freezing/thawing of embryos on the process of recovery of the embryo and its subsequent implantation.ResultsFreezing/thawing is a way to activate the endogenous survival and repair responses in preimplantation embryos. Several molecular mechanisms can explain the higher success rate of ET using thawed embryos compared to fresh ET in women of advanced reproductive age, the higher miscarriage rate in cases of thawed blastocyst ET compared to thawed ET at early cleavage embryo, and the higher perinatal parameters of born children after thawed ET. Embryo thawing induces a stress. Controlled stress is not necessarily detrimental, because it generates a phenomenon that is counteracted by several known biological responses aimed to repair mitochondrial damage of membrane and protein misfolding. The term for favorable biological responses to low exposures to stress is called hormesis.ConclusionsThis thesis will summarize the role of cryopreservation in the activation of a hormetic response, preserving the mitochondrial function, improving survival, and having an impact on the process of implantation, miscarriage, and the development of pregnancy.

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Developmental assessment of human vitrified-warmed blastocysts based on oxygen consumption

Cited by 49 publications

References 23 publications

A closed system supports the developmental competence of human embryos after vitrification

A closed system supports the developmental competence of human embryos after vitrification

Unconventional embryo selection strategies: a look into the future

Theory about the Embryo Cryo‐Treatment

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