Developmental blockage of mouse embryos caused by fatty acids

Nonogaki, Takafumi; Noda, Yoichi; Goto, Yasuo; Kishi, Junji; Mori, Takahide

doi:10.1007/bf02215713

Cited by 64 publications

(40 citation statements)

References 26 publications

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“…In the present study, maturation of oocytes in the presence of LA at 50 mM (which has no significant inhibitory effect on oocyte maturation and cumulus expansion) and 100 mM significantly decreased the cleavage and blastocyst rate. In a previous study, similar effects were reported when mouse pronuclear and two-cell stages were treated by LA in vitro (Nonogaki et al 1994). These authors also showed that the addition of antioxidants to embryo culture media could attenuate the rate of developmental blockage, suggesting that the effect was caused by damage of the cells by lipid peroxidation.…”

Section: Discussionsupporting

confidence: 69%

Impact of linoleic acid on bovine oocyte maturation and embryo development

Marei¹,

Wathes²,

2010

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Linoleic acid (LA; 18:2 n-6) is the most abundant fatty acid in bovine follicular fluid, and it was previously reported that LA concentration significantly decreases when follicle size increases. This suggests that LA may have a role in the regulation of oocyte maturation. The present study investigated the effect of LA supplementation on bovine oocyte maturation and early embryo development in vitro. Treatment of cumulus-oocyte complexes (COCs) with LA significantly inhibited cumulus cell expansion and retarded development of the oocytes to the metaphase II (MII) stage in a dose-dependent manner. This effect was reversible, and the oocytes developed to the MII stage after extended culture in the absence of LA. Treatment of COCs with LA also resulted in a significantly lower percentage of cleaved embryos and blastocyst yield. Furthermore, COCs treated with LA had significant effects compared with controls in i) increasing prostaglandin E 2 concentration in the medium, ii) decreasing intracellular cAMP at 6 and 24 h of maturation and iii) decreasing phosphorylation of the MAPK1 and 3 at 24 h, and AKT at 6 h of maturation. In conclusion, LA supplementation to bovine oocytes during maturation altered the molecular mechanisms regulating oocyte maturation and resulted in decreased percentage of oocytes at MII stage and inhibition of the subsequent early embryo development. These data provide evidence for adverse effects of LA on oocyte development, which can be associated with dietary increased level of LA in the follicular fluid and the decline in fertility in farm animals and human.Reproduction (2010) 139 979-988

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Section: Discussionsupporting

confidence: 69%

Impact of linoleic acid on bovine oocyte maturation and embryo development

Marei¹,

Wathes²,

2010

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“…Fatty acyl residues have previously been shown to be associated with oocyte maturation and fertility ( 18 ) as well as with embryo development ( 19,35,41,42 ) in mammalian species. As an example, storage and membrane lipid con- known to present the high sensitivity needed to detect the few picomols of lipids present in these samples.…”

Section: Discussionmentioning

confidence: 99%

Single embryo and oocyte lipid fingerprinting by mass spectrometry

Ferreira

Saraiva

Catharino

et al. 2010

Journal of Lipid Research

120

104

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This article is available online at http://www.jlr.org ing in cell membranes. Specifi c functions and variations of the various phospholipids (PL), the most abundant lipids in eukaryotic cell membranes, are, however, still poorly understood ( 1 ). A diversity of PL in a fi nely balanced equilibrium is used by cells to construct stable and functional membranes, and PL composition determines most of the physico-chemical cell membrane properties such as fl uidity, permeability and thermal phase behavior ( 2 ).Knowledge of the function of lipids within the cell has benefi ted from the development of increasingly sensitive and selective analytical techniques, particularly those based on mass spectrometry ( 3, 4 ). Among these techniques, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) ( 5 ) has been very successful in studies of the compositions of lipids and other crucial biological molecules. MALDI-MS has allowed direct analysis of complex and unfractionated samples, such as the study of peptide profi les below the level of a single cell ( 6 ). In lipidomics, MALDI-MS has provided fast and simple acquisition of mass spectra with lipid profi les of cells, tissues and body fl uids ( 7 ).MALDI-MS lipid fi ngerprinting can, for example, help studies aimed at understanding the effect of membrane lipid composition on cell membrane behavior after temperature changes. This knowledge is essential for cryopreservation studies of a variety of cells, including oocytes The double molecular layer of polar lipids is a marvelous architectural feature of exquisite biological engineerThis work was supported by the Brazilian research foundations FAPESP (Grant 2008/10756-7) and CNPq.

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“…Nonogaki et al (1994) observed an arrest in embryo development after exposure of mouse zygotes to high concentrations of PUFAs (linoleic and α-linolenic acid). Also, oocyte exposure to a maternal environment enriched with n-3 PUFA (diet supplemented with n-3) resulted in altered mitochondrial distribution and calcium levels and increased ROS production (Wakefield et al, 2008).…”

Section: Fa Composition Of Ff In Relation To the Diet And Its Influenmentioning

confidence: 98%

Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes

Warzych¹,

Cieślak²,

Pawlak³

et al. 2011

Vet. Med.

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Metabolomics focused on reproduction have been the subject of special interest in the past decade. Metabolite profiling provides detailed information on the status of follicular fluid and follicular cells which accompany the growing oocyte. Although nutrients present in the diet reach oocytes via the follicular fluid, it is not evident whether oocyte/embryo quality can be predicted based on the follicular fluid composition. Since this phenomenon has not yet been investigated in the pig, the aim of the present study was to investigate associations between diet composition and (1) selected markers related to developmental potential of oocytes (brilliant cresyl blue -BCB test, relative transcript abundance of EEF1A1 and ATP5A1 marker genes) and (2) fatty acid profile in the follicular fluid. Gilts were fed control and experimental diets which differed in composition and fatty acid profiles. The experimental diet used in this study comprised mainly locally grown grains (barley and rye) traditionally used for fattening pigs in Poland. Cumulus-oocyte complexes were aspirated from individual pairs of ovaries after animal slaughter, evaluated morphologically and subjected to the BCB test. Relative transcript abundance for the two marker genes was monitored by real-time PCR in oocytes of both categories (BCB + and BCB -). Fatty acid profile in follicular fluid was analyzed by gas chromatography. We show that the experimental diet rich in n-3 fatty acid significantly influences fatty acid composition of the follicular fluid. The fatty acid profile of the follicular fluid of gilts fed the experimental diet differed from that of the control females. The content of saturated fatty acids was higher in the experimental group, whereas unsaturated and polyunsaturated fatty acids were more abundant in the control group (P < 0.05). With regard to individual fatty acids, only C16:0 (palmitic acid), C18:2n-6 (linoleic acid) and C22:6 (docosahexaenoic acid) differed significantly. The abundance of ATP5A1 mRNA was influenced neither by diet composition nor by oocyte category (BCB + /BCB -) whereas that of the EEF1A1 was affected by both factors. Since higher mRNA level of the EEF1A1 gene was noted in BCB + oocytes this may be considered as a marker of oocyte quality in the pig. Maternal nutrition is one of the main factors affecting developmental competence of mammalian oocytes and embryos. Diet composition may alter the maternal environment of the oocyte and embryo growth through metabolic and endocrine modifications. Zak et al. (1997) showed that feed intake influenced the meiotic competence of porcine oocytes. They claimed that maternal under-nutrition may reduce the follicle's ability to support maturation of oocytes and thus their quality. Follicular fluid (FF) as an endogenous medium participates in transmitting nutrition signals to the

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Developmental blockage of mouse embryos caused by fatty acids

Abstract: These results suggest that lipid radicals can easily be generated in early stage embryos and that blastomeres are among the cells vulnerable to the damage by lipid peroxidation.

Cited by 64 publications

References 26 publications

Impact of linoleic acid on bovine oocyte maturation and embryo development

Impact of linoleic acid on bovine oocyte maturation and embryo development

Single embryo and oocyte lipid fingerprinting by mass spectrometry

Maternal nutrition affects the composition of follicular fluid and transcript content in gilt oocytes

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