Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm

Guzmán, Luis; Ortega-Hrepich, Carolina; Albuz, F. K.; Verheyen, Greta; Devroey, Paul; Smitz, Johan; Vos, Michel De

doi:10.1016/j.fertnstert.2012.01.114

Cited by 56 publications

(40 citation statements)

References 40 publications

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“…Larger follicles (with 6 mm diameters or more) were easier structures in which to perform repeated flushes than smaller follicles and were more easily visualized after flushing because of the extrafollicular fluid introduced into the ovary. Larger follicles were likely to produce more useful oocytes [8,13]. This could contribute to a better quality embryos being obtained if the ST needle is used rather than the Cook needle.…”

Section: Discussionmentioning

confidence: 99%

“…A good embryo on day 3 was defined as originating from an oocyte that matured within 10 h of aspiration and by day 3 (64 to 72 h after aspiration) had developed into a 7 or 8 cell embryo with less than 10 % fragmentation and with relatively uniform blastomeres [14]. The surgical time was defined as the time interval from when the embryologist 1 The choice of using low dose gonadotrophins (50 U FSH for 4 to 6 days) had the objective of increasing antral follicle diameters [8,13] without pushing past the 12 mm largest AF diameter size target. The value of this approach has been affirmed in recent publications [5,10].…”

Section: Methodsmentioning

confidence: 99%

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A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM

Rose¹,

Laky²

2013

J Assist Reprod Genet

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Purpose This study compared the impact of using the Steiner-Tan pseudo double lumen needle for antral follicle oocyte retrieval to using a conventional non-flushing needle. The Steiner-Tan needle has a much smaller dead space than the needles commonly used for IVM oocyte retrievals. Methods This was a retrospective cohort study. The patient population was determined by the time period in which a patient underwent IVM in a single physician's IVF practice. The following data was abstracted from clinical and embryology records: oocytes retrieved, oocytes matured, early maturing oocytes, oocytes fertilized, embryo quality measures, retrieval time, needle punctures, clot formation, and clinical pregnancy rate. Results The Steiner-Tan needle did not increase the number of oocytes retrieved. It also did not increase the time required for retrieval. However, flushing of antral follicles significantly decreased clot formation in fluid aspirates. Use of the Steiner-Tan needle also significantly decreased the number of vaginal needle punctures during each case. There was a trend toward improved embryo quality, but statistical power was inadequate to show a difference. Conclusions The primary benefit of the Steiner-Tan needle was on the embryological aspects of IVM. Decreased blood and blood clots in the aspirates made an IVM retrieval more like conventional IVF for the embryologist. The patient also experienced less tissue trauma without increasing anesthesia or surgical time. There was no improvement in the number of oocytes retrieved, but based on the results, we hypothesized that oocytes were more commonly retrieved from slightly large follicles than when using a routine needle.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM

Rose¹,

Laky²

2013

J Assist Reprod Genet

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“…Dramatic effects of zinc deficiency have been uncovered in oocytes (Kim et al 2010, Suzuki et al 2010b) and cumulus cells (Tian & Diaz 2012) during the periovulatory transition. Currently, the success of human IVM is highly variable (4-35% clinical pregnancy/ transfer) (Cha et al 1991, Trounson et al 1994, Child et al 2001, Gremeau et al 2012, Guzman et al 2012. The ability to reliably mature oocytes in vitro would reduce reliance on human chorionic gonadotropin (hCG) injections which are associated with ovarian hyperstimulation syndrome (Aboulghar & Mansour 2003).…”

Section: Zinc Homoeostasis In the Oocytementioning

confidence: 99%

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Lisle¹,

Anthony²,

Randall³

et al. 2013

Reproduction

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Zinc increases in the oocyte during maturation and is required for progression and completion of meiosis. The objective of this study was to determine whether cumulus cells regulate the levels of free intracellular zinc in the oocyte during maturation. In the cumulus-oocyte complex (COC) the relative level of free intracellular zinc was almost fourfold higher in cumulus cells compared with the resident germinal vesicle-stage oocyte. Removal of cumulus cells caused a fourfold increase in intracellular zinc in the oocyte by 1 h after cumulus cell removal, but subsequent coculture of denuded oocytes with COC decreased free intracellular zinc in the oocyte by 65%. Thus, cumulus cells suppress free intracellular zinc in the oocyte. The mRNA transcripts for the zinc transporter proteins Slc39a6, Slc39a8, Slc39a9, Slc39a10, Slc39a12, Slc30a2, Slc30a4, Slc30a5 and Slc30a8 mRNAs were higher in oocytes, while Slc39a1, Slc39a7, Slc39a13, Slc39a14, Slc30a6, Slc30a7 and Slc30a9 mRNAs were higher in cumulus cells. Thus a complex zinc transport network is present in the COC. Pretreatment with epidermal growth factor for 4 h abolished the ability of COCs to restrict free intracellular zinc in denuded oocytes. Coculture of denuded metaphase II oocytes with COC lowers free intracellular zinc in mature oocytes. Oocytes matured in vivo or oocytes from older mice had lower levels of free intracellular zinc than oocytes matured in vitro or from younger mice. Thus, a precise mechanism for regulating oocyte zinc homeostasis has been uncovered in the COC that is disrupted with increasing age or by removal of cumulus cells.

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“…En pacientes con SOP en quienes no se administró hCG, De Vos y cols reportan una tasa de embarazo con transferencia embrionaria en ciclos frescos inferiores al 10%, la cual aumenta de forma significativa al transferir embriones desvitrificados en ciclos diferidos con preparación endometrial artificial de 9,4% a 31,8% (p=0,033)(6), y de 5,4% a 37,5% (p=0,004) (21).…”

Section: Discussionunclassified

Maduración in vitro de ovocitos humanos: una opción para preservar la fertilidad de las mujeres. Reporte del primer caso realizado en Chile

Ortega-Hrepich¹,

M²,

Arguello³

et al. 2014

Rev. chil. obstet. ginecol.

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Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm

Cited by 56 publications

References 40 publications

A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM

A comparison of the Cook single lumen immature ovum IVM needle to the Steiner-Tan pseudo double lumen flushing needle for oocyte retrieval for IVM

Oocyte–cumulus cell interactions regulate free intracellular zinc in mouse oocytes

Maduración in vitro de ovocitos humanos: una opción para preservar la fertilidad de las mujeres. Reporte del primer caso realizado en Chile

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