Developmental capacity of vitrified immature porcine oocytes following ICSI: effects of cytochalasin B and cryoprotectants

Fujihira, Takuma; Kishida, Rie; Fukui, Yutaka

doi:10.1016/j.cryobiol.2004.08.004

Cited by 82 publications

(76 citation statements)

References 13 publications

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“…Vitrification is a simple, convenient, and efficient method for embryo and oocyte cryopreservation in both animal and human models (Fujihira et al 2004, Campos-Chillò n et al 2009, Cao et al 2009, Keros et al 2009, Selman et al 2009, Zhou et al 2010. However, data on ovarian tissue vitrification are still limited, and the results are often conflicting and controversial (Choi et al 2008, Isachenko et al 2009, Kagawa et al 2009.…”

Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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“…Hardening of the zona pellucida caused by the cryopreservation process has been observed in mammalian oocytes [44,45] which may provide a reasonable explanation for the reduced penetration rates. To resolve this problem ICSI seems to be the optimum procedure to generate embryos from cryopreserved oocytes [22,46]; however, in pigs, frequent failure of male pronuclear formation still limits the success of this method [47][48][49]. Furthermore, we have observed increased frequency of male pronuclear formation failure in vitrified M-II stage oocytes after being penetrated by sperm demonstrating that successful penetration may not ensure normal embryogenesis [27].…”

Section: Matured Oocytes; High Survival But Low Developmental Abilitymentioning

confidence: 99%

“…On the other hand, several vitrification methods have been applied for the preservation of immature and mature porcine oocytes such as open pulled straw (OPS) [20,21], Cryotop [22][23][24] and solid surface vitrification (SSV) [25,26]. They all provided surviving oocytes at various rates.…”

Section: Which Is the Optimum Methods For Oocyte Cryopreservation?mentioning

confidence: 99%

“…Dev. 58: [17][18][19][20][21][22][23][24] 2012) B ecause of the worldwide adaptation of western breeds and breeding technology, several indigenous pig breeds have been put on the verge of extinction by the end of the 20th century including some European and Asian breeds [1,2]. Nevertheless, recent increase in demand of some indigenous European pig breeds such as the Hungarian Mangalica and the Spanish Iberico breeds and their growing market underline the importance of the preservation of genetic diversity in pigs [3,4].…”

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confidence: 99%

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Factors Affecting Cryopreservation of Porcine Oocytes

2012

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“…As a consequence, cryopreservation of porcine oocytes by conventional slow cooling remains unsuccessful [9]. Some success in cryopreservation of immature and in vitro matured (IVM) porcine oocytes has been achieved by application of vitrification methods [10][11][12]. Despite recent improvements in survival rates, the developmental competence after vitrification of porcine IVM oocytes is still very poor [13,14].…”

mentioning

confidence: 99%

Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro

Somfai

Kashiwazaki

Ozawa

et al. 2008

Journal of Reproduction and Development

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Abstract. The aim of the present study was to investigate the effects of centrifugation pretreatment on the viability and nuclear status of porcine in vitro matured (IVM) oocytes and on the developmental competence of in vitro fertilized (IVF) oocytes (zygotes) after cryopreservation by vitrification (Solid Surface Vitrification; SSV). Mature oocytes having the first polar body after IVM and zygotes having the second polar body at 10 h after IVF were centrifuged at 10,000 × g at 37 C for 20 min and then subjected to SSV. Their viability was evaluated by morphological appearance and fluorescein diacetate staining. The nuclear status of oocytes was evaluated 6 h after vitrification. The developmental ability to the blastocyst stage of vitrified zygotes was evaluated after 6 days of in vitro culture. Although centrifugation did not damage the oocytes directly, it drastically reduced the rate of live oocytes after SSV. The rates of vitrification-induced parthenogenetic activation were similar in both centrifuged and non-centrifuged oocytes (42.4 and 47.4%, respectively). Centrifugation had no significant effects on the viability of pronuclear oocytes. The development of vitrified zygotes to the blastocyst stage was significantly lower than that of the control irrespective of centrifugation pretreatment. There was no difference in the cleavage and blastocyst rates between the control and centrifuged zygotes after vitrification. There was also no difference in the total cell numbers of blastocysts between the control and centrifuged zygotes irrespective of vitrification. These results reveal that, in IVM porcine oocytes, centrifugation pretreatment is highly detrimental to cryotolerance; however, in zygotes, it has only a slight effect on viability and does not alter the developmental competence of surviving zygotes. Key words: Centrifugation, Oocyte, Pig, Vitrification, Zygote (J. Reprod. Dev. 54: [149][150][151][152][153][154][155] 2008) he oocytes and early embryos of cattle and pigs are known to contain large numbers of cytoplasmic lipid droplets, which have an important role in their energy metabolism [1]. Nevertheless this characteristic has a huge impact on their availability for certain methods in animal biotechnology. For instance, it is known that a large number of lipid droplets causes the sensitivity of porcine embryos to low temperatures, which makes them very difficult to cryopreserve [2]. Also, the high lipid content that makes the cytoplasm of oocytes and early embryos dark blocks the visibility of organelles, which is essential for certain techniques such as microinjection of DNA into pronuclei [3].Polarization of lipid droplets by centrifugation is an efficient method to increase visibility of pronuclei [4]. Besides its importance in the production of transgenic cattle and pigs, centrifugation can be also used to confirm the onset of oocyte activation by the existence of pronuclei [5] or to separate monospermic zygotes from polyspermic ones [6]. Moreover, after centrifugation treatment, polarized ...

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Developmental capacity of vitrified immature porcine oocytes following ICSI: effects of cytochalasin B and cryoprotectants

Cited by 82 publications

References 13 publications

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Factors Affecting Cryopreservation of Porcine Oocytes

Effect of Centrifugation Treatment before Vitrification on the Viability of Porcine Mature Oocytes and Zygotes Produced In Vitro

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