2001
DOI: 10.1002/neu.1073
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Developmental changes in BDNF protein levels in the hamster retina and superior colliculus

Abstract: Quantitative studies of ontogenetic changes in the levels of brain-derived neurotrophic factor (BDNF) mRNA and its effector, BDNF protein, are not available for the retinal projection system. We used an electrochemiluminescence immunoassay to measure developmental changes in the tissue concentration of BDNF within the hamster retina and superior colliculus (SC). In the SC, we first detected BDNF (about 9 pg/mg tissue) on embryonic day 14 (E14). BDNF protein concentration in the SC rises about fourfold between … Show more

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Cited by 32 publications
(28 citation statements)
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“…Electrochemiluminescent immunoassay (ECLIA; Blackburn et al, 1991) is an established (Ma et al, 1998;Pollock et al, 2001;Frost et al, 2001) and powerful technique for measuring rare proteins. The strengths of the technique are that ECLIA rapidly detects very small quantities of protein (Ͻ0.1 pg) in large volumes, the electrochemiluminescent (ECL) species is regenerated, and there is signal amplification and a very high signal to noise ratio.…”
Section: Electrochemiluminescent Immunoassaymentioning
confidence: 99%
“…Electrochemiluminescent immunoassay (ECLIA; Blackburn et al, 1991) is an established (Ma et al, 1998;Pollock et al, 2001;Frost et al, 2001) and powerful technique for measuring rare proteins. The strengths of the technique are that ECLIA rapidly detects very small quantities of protein (Ͻ0.1 pg) in large volumes, the electrochemiluminescent (ECL) species is regenerated, and there is signal amplification and a very high signal to noise ratio.…”
Section: Electrochemiluminescent Immunoassaymentioning
confidence: 99%
“…While each neurotrophin has been reported to influence visual system development to some extent, BDNF has repeatedly emerged as a particularly important neurotrophic signal that influences multiple phases of vertebrate RGC development including survival, morphological differentiation of axons and dendrites, synapse formation, and regeneration (Bahr, 2000;Frost et al, 2001;Isenmann et al, 2003;. The temporal and spatial expression patterns of BDNF and TrkB receptors within the developing visual system indicate that BDNF is available to influence important aspects of RGC differentiation, including their morphological maturation.…”
Section: S Cohen-cory and B Lommentioning
confidence: 99%
“…The temporal and spatial expression patterns of BDNF and TrkB receptors within the developing visual system indicate that BDNF is available to influence important aspects of RGC differentiation, including their morphological maturation. BDNF and its receptor TrkB are highly expressed in the visual system of most vertebrate species examined, from fish to mammals (Cellerino and Kohler, 1997;Cohen-Cory et al, 1996;Cohen-Cory and Fraser, 1994;Duprey-Diaz et al, 2002;Frost et al, 2001;Garcia et al, 2003;Hallbook et al, 1996;Hashimoto and Heinrich, 1997;Herzog et al, 1994;Herzog and von Bartheld, 1998;Jelsma et al, 1993;Perez and Caminos, 1995). BDNF was initially characterized for its ability to promote survival of cultured RGCs (Cohen-Cory and Fraser, 1994;Johnson et al, 1986;Rodriguez-Tebar et al, 1989), but BDNF does not modulate RGC programmed cell death in vivo because RGC numbers in TrkB-or BDNF-deficient mice are similar to wild types Pollock et al, 2003;Rohrer et al, 2001).…”
Section: Neurotrophins and Visual System Developmentmentioning
confidence: 99%
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“…In development, innervation of RGCs correlates with the spatial and temporal expression of BDNF in the visual system [8385]. For example, in hamster, RGCs start populating the retina at embryological day 10 (E10), extend axons through the optic nerve, and arrive at the SC by E13, a time when BDNF protein levels are very low in both retina and SC [83,86,87].…”
Section: 0 Bdnf/trkb In Developing Retinamentioning
confidence: 99%