Developmental changes in glucose transport, lipid composition, and fluidity of jejunal BBM

Vázquez, Carmen M.; Rovira, Nativitat; Ruı́z-Gutı́errez, Valentina; Planas, Joana M.

doi:10.1152/ajpregu.1997.273.3.r1086

Cited by 28 publications

(27 citation statements)

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“…The use of BBMV from the isolated jejunal enterocytes confirmed the results obtained in BBMV isolated from mucosa by Shehata et al (22) and Vá zquez et al (29). In the present study, methyl ␣-D-glucopyranoside transport per milligram of BBM protein was 40% higher at 2 days than at 5 wk (P Ͻ 0.01).…”

Section: Discussionsupporting

confidence: 91%

“…This value was well correlated with the decline observed in methyl ␣-D-glucopyranoside transport (40%) and corroborates the decrease in phlorizin binding during chicken development reported by Vá zquez et al (29). Similarly, an age-related reduction in transport rates that could be explained by a decrease in the density of transporters was found in mice (7) and lambs (23).…”

Section: Discussionsupporting

confidence: 90%

“…Consistent with our previous reports (25,29), in the present study we selected chickens of only two ages: 2 days, representing the newly hatched period when maximal uptake is found, and 5 wk, representing the values attained in adulthood.…”

Section: Discussionmentioning

confidence: 85%

“…The maximal capacity per tissue weight to transport monosaccharides is reached the day after hatch and declines afterward until attainment of adult levels at the age of 5 wk (18,25). This pattern occurs not only in chickens (19,29) but also in various species of mammals (2,23).…”

Section: Discussionmentioning

confidence: 98%

“…The purity of the BBMV was determined by assaying for sucrase activity and the ouabain-sensitive Na ϩ -K ϩ -activated ATPase, as described previously (29).…”

Section: Animalsmentioning

confidence: 99%

See 4 more Smart Citations

Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

Barfull

Garriga

Mitjans

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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To evaluate the effect of age on sugar transport, we determined the uptake of methyl α-d-glucopyranoside and the abundance of the Na+-d-glucose cotransporter (SGLT1) in jejunal brush-border membrane (BBM) vesicles of 2-day- and 5-wk-old chickens. Methyl α-d-glucopyranoside transport per BBM protein was 40% lower in adults than in newly hatched chickens. This finding was matched by parallel declines in site density of SGLT1, which were detected by Western blot. The immunohistochemical study showed that SGLT1 was exclusively located in the BBM of enterocytes along the entire villus and was absent in the crypt in both age groups, and there was an 11-fold increase in the total absorptive area during development. Northern blot studies of the abundance of SGLT1 mRNA showed similar levels for the groups studied. We conclude that the age-related decline in Na+-dependent hexose transport per unit of BBM protein in the chicken jejunum is due to a reduction in the density of SGLT1 cotransporter and is regulated by a posttranscriptional mechanism.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 98%

“…The purity of the BBMV was determined by assaying for sucrase activity and the ouabain-sensitive Na ϩ -K ϩ -activated ATPase, as described previously (29).…”

Section: Animalsmentioning

confidence: 99%

See 3 more Smart Citations

Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

Barfull

Garriga

Mitjans

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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Comparative Digestive Physiology

Karasov

Douglas

2013

Comprehensive Physiology

263

221

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In vertebrates and invertebrates, morphological and functional features of gastrointestinal (GI) tracts generally reflect food chemistry, such as content of carbohydrates, proteins, fats, and material(s) refractory to rapid digestion (e.g., cellulose). The expression of digestive enzymes and nutrient transporters approximately matches the dietary load of their respective substrates, with relatively modest excess capacity. Mechanisms explaining differences in hydrolase activity between populations and species include gene copy number variations and single-nucleotide polymorphisms. Transcriptional and posttranscriptional adjustments mediate phenotypic changes in the expression of hydrolases and transporters in response to dietary signals. Many species respond to higher food intake by flexibly increasing digestive compartment size. Fermentative processes by symbiotic microorganisms are important for cellulose degradation but are relatively slow, so animals that rely on those processes typically possess special enlarged compartment(s) to maintain a microbiota and other GI structures that slow digesta flow. The taxon richness of the gut microbiota, usually identified by 16S rRNA gene sequencing, is typically an order of magnitude greater in vertebrates than invertebrates, and the interspecific variation in microbial composition is strongly influenced by diet. Many of the nutrient transporters are orthologous across different animal phyla, though functional details may vary (e.g., glucose and amino acid transport with K+ rather than Na+ as a counter ion). Paracellular absorption is important in many birds. Natural toxins are ubiquitous in foods and may influence key features such as digesta transit, enzymatic breakdown, microbial fermentation, and absorption

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Decreased monosaccharide transport in renal brush-border membrane vesicles of spontaneously hypertensive rats

Mate

Hermosa

Barfull

et al. 2000

Cellular and Molecular Life Sciences (CMLS)

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Na(+)-dependent D-glucose and D-galactose transport were studied in brush-border membrane vesicles (BBMVs) from kidney cortex isolated from both spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto (WKY) rats. Initial rates and accumulation ratios of Na(+)-dependent D-glucose and D-galactose transport were significantly lower in SHR compared with WKY, the observed decreases being similar for both substrates. To explain the reduction in sugar transport by renal BBMVs, the density of Na(+)-dependent sugar cotransporters was studied in BBMVs from kidney cortex isolated from SHR and WKY rats. Phlorizin-specific binding and Western blot analysis indicated a reduction in the density of the cotransporters in SHR relative to WKY rats. This reduction was similar to those found for the initial rates and accumulation ratios for D-glucose and D-galactose in SHR. Na+ uptake, studied using 22Na+, was significantly increased in SHR, so the observed reduction in sugar transport could be due to disruption of the Na+ gradient between renal BBMVs in SHR. Furthermore, a significant decrease in the activity of Na(+)-K(+)-ATPase was observed in SHR. In conclusion, changes in the density of the Na(+)-dependent sugar cotransporter and in the Na+ gradient across the brush-border membranes might be involved in the observed reduction in sugar transport by renal BBMVs from SHR.

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Developmental changes in glucose transport, lipid composition, and fluidity of jejunal BBM

Cited by 28 publications

References 0 publications

Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

Comparative Digestive Physiology

Decreased monosaccharide transport in renal brush-border membrane vesicles of spontaneously hypertensive rats

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Developmental changes in glucose transport, lipid composition, and fluidity of jejunal BBM

Cited by 28 publications

References 0 publications

Ontogenetic expression and regulation of Na+-d-glucose cotransporter in jejunum of domestic chicken

Ontogenetic expression and regulation of Na+-d-glucose cotransporter in jejunum of domestic chicken

Comparative Digestive Physiology

Decreased monosaccharide transport in renal brush-border membrane vesicles of spontaneously hypertensive rats

Contact Info

Product

Resources

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Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken

Ontogenetic expression and regulation of Na⁺-d-glucose cotransporter in jejunum of domestic chicken