Developmental changes in glutamate receptor stimulated inositol phospholipid metabolism and 45Ca2+-accumulation in posthatch chicken forebrain

Kavanagh, Jacinta.M.; Bunn, Stephen J.; Boyd, Teresa L.; Rostas, John A. P.

doi:10.1016/0304-3940(95)11749-m

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…The microslices were incubated at 371C and continually oxygenated throughout the experimental procedure. 16 Excitotoxic stimulation. Microslices were stimulated with 100 mmol/L AMPA, 100 mmol/L NMDA þ 50 mmol/L glycine, or 2.5 mmol/L glutamate þ 50 mmol/L glycine (final concentration in 150 mL of Krebs buffer; control tissue received the same volume of Krebs without compounds).…”

Section: Micropunch Homogenates Fifteen-week-old Male Shr Wky Ormentioning

confidence: 99%

αCaMKII is Differentially Regulated in Brain Regions that Exhibit Differing Sensitivities to Ischemia and Excitotoxicity

Skelding

Spratt

Fluechter

et al. 2012

J Cereb Blood Flow Metab

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Different brain regions exhibit differing sensitivities to ischemia/excitotoxicity. Whether these differences are due to perfusion or intrinsic factors has not been established. Herein, we found no apparent association between sensitivity to ischemia/excitotoxicity and the level of expression or basal phosphorylation of calcium/calmodulin-stimulated protein kinase II (αCaMKII) or glutamate receptors. However, we demonstrated significant differences in CaMKII-mediated responses after ischemia/excitotoxic stimulation in striatum and cortex. In vivo ischemia and in vitro excitotoxic stimulation produced more rapid phosphorylation of Thr253-αCaMKII in striatum compared with cortex, but equal rates of Thr286-αCaMKII phosphorylation. Phosphorylation by CaMKII of Ser831-GluA1 and Ser1303-GluN2B occurred more rapidly in striatum than in cortex after either stimulus. The differences between brain regions in CaMKII activation and its effects were not accounted for by differences in the expression of αCaMKII, glutamate receptors, or density of synapses. These results implicate intrinsic tissue differences in Thr253-αCaMKII phosphorylation in the differential sensitivities of brain regions to ischemia/excitotoxicity.

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Section: Micropunch Homogenates Fifteen-week-old Male Shr Wky Ormentioning

confidence: 99%

αCaMKII is Differentially Regulated in Brain Regions that Exhibit Differing Sensitivities to Ischemia and Excitotoxicity

Skelding

Spratt

Fluechter

et al. 2012

J Cereb Blood Flow Metab

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“…Nevertheless, some molecular and cellular changes are so widespread that they can be measured in whole forebrain, e.g. changes in the functional properties of NMDA and metabotropic glutamate receptors [Kavanagh et al, , 1995 and an increased accumulation of Ca 2+ /calmodulin-dependent protein kinase II at the postsynaptic density (PSD) [Weinberger and Rostas, 1988].…”

Section: Introductionmentioning

confidence: 99%

Maturational Changes in the Subunit Composition of AMPA Receptors and the Functional Consequences of Their Activation in Chicken Forebrain

Migues

Cammarota²,

Kavanagh³

et al. 2006

Dev Neurosci

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AMPA receptors play a critical role in synaptic plasticity and brain development. Here we show that Ca²⁺ uptake in response to AMPA receptor activation decreases dramatically during maturation in chicken brain microslices without a change in tissue AMPA receptor content. We found that during maturation the relative concentration of GluR2 subunits increased, the concentration of the AMPA receptor-associated scaffold proteins SAP97 and GRIP decreased and that depolarization increased GluR1 phosphorylation at Ser831 in subcellular fractions enriched in postsynaptic densities at 2 weeks but not at 10 weeks. These changes are all consistent with a decreased Ca²⁺ entry through AMPA receptor channels in response to receptor activation and may account for the changes in the functional properties of the receptor, which are thought to underlie, at least in part, the physiological changes that occur with maturation.

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“…Based on previous biochemical, electrophysiological, and ultrastructural studies in our laboratory we have shown that in chicken forebrain the synapse formation phase is complete by 10–14 days and by 8–10 weeks post‐hatch synapses and neurons have attained adult ultrastructural and biochemical properties (Rostas et al. 1984; Rostas and Dunkley 1992; Kavanagh et al. 1995; Margrie et al.…”

Section: Methodsmentioning

confidence: 93%

Molecular changes in the intermediate medial mesopallium after a one trial avoidance learning in immature and mature chickens

Atkinson

Migues

Hunter

et al. 2008

Journal of Neurochemistry

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Abbreviations used: CaMKII, calcium-calmodulin stimulated/dependent protein kinase II; DTAL, discriminative taste aversion learning; ERK, extracellular signal regulated kinase; GluR1, glutamate receptor subunit 1; IMM, intermediate medial mesopallium; ITM, intermediate-term memory; LTM, long-term memory; LTP, long term potentiation; MAPK, mitogen activated protein kinase; MeA, methylanthranilate; PAL, passive avoidance learning; PSD, post-synaptic density; pSer831-GluR1, phosphorylation of GluR1 at Ser831; pT286-CaMKII, phosphorylation of CaMKII at Thr286; RSPI, relative stoichiometry of phosphorylation index; TBS-T, Tris-buffered saline containing 0.1% Tween-20. AbstractBecause brain maturation in chickens is protracted and occurs well after the major developmental period of synaptogenesis, chicken forebrain is suitable to investigate whether the molecular mechanisms underlying memory consolidation are different in immature and mature animals. We have used antibodies and western blotting to analyze subcellular fractions from the intermediate medial mesopallium region of 14-day and 8-week chicken forebrain prepared 0, 45, and 120 min after learning a discriminative taste avoidance task. At both ages learning induced changes in the phosphorylation of the glutamate receptor subunit 1 at Ser831, the levels of calcium-calmodulin stimulated/dependent protein kinase II and the phosphorylation of calcium-calmodulin stimulated/ dependent protein kinase II at Thr286 were observed only in the fraction enriched in post-synaptic densities. The changes were of the same type at the two ages but occurred faster in mature animals. The changes in extracellular signal regulated kinase and phosphorylated-extracellular signal regulated kinase were more complex with different subcellular fractions showing different patterns of change at the two ages. These results imply that the molecular changes induced by learning a behavioral task are faster in mature than immature brain and may involve a different balance of intracellular signaling pathways.

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Developmental changes in glutamate receptor stimulated inositol phospholipid metabolism and 45Ca2+-accumulation in posthatch chicken forebrain

Cited by 7 publications

References 15 publications

αCaMKII is Differentially Regulated in Brain Regions that Exhibit Differing Sensitivities to Ischemia and Excitotoxicity

αCaMKII is Differentially Regulated in Brain Regions that Exhibit Differing Sensitivities to Ischemia and Excitotoxicity

Maturational Changes in the Subunit Composition of AMPA Receptors and the Functional Consequences of Their Activation in Chicken Forebrain

Molecular changes in the intermediate medial mesopallium after a one trial avoidance learning in immature and mature chickens

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