Developmental changes in glutathione S-transferase isoforms expression and activity in intrasplenic fetal liver tissue transplants in rats

Lupp, Amelie; Anschütz, Tino; Lindström-Seppä, Pirjo; Müller, Dieter

doi:10.1078/0940-2993-00286

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…However, the expression of CK19 mRNA appeared soon after culture, reached a plateau after 5 days in spheroids, and was maintained thereafter within the collagen gels ( Figure 3, data not shown). The gene ex- pression of another bile duct marker, GST-P, 29 also dramatically increased and persisted in cultured hepatocytes ( Figure 3). Although DMBT1 protein has been reported to be positive in atypical ductular reaction and negative in normal hepatocytes and bile ducts, 23 its mRNA was clearly detected in normal portal tissues (Figure 3).…”

Section: Gene Expression Of Bile Duct-specific Proteins As Well As Omentioning

confidence: 96%

Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro

Nishikawa¹,

Doi²,

Watanabe³

et al. 2005

The American Journal of Pathology

103

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We investigated the mechanism of phenotypic plasticity of hepatocytes in a three-dimensional organoid culture system, in which hepatocytic spheroids were embedded within a collagen gel matrix. Hepatocytes expressed several bile duct markers including cytokeratin (CK) 19 soon after culture and underwent branching morphogenesis within the matrix in the presence of insulin and epidermal growth factor. Cultured hepatocytes did not express Delta-like, a specific marker for oval cells and hepatoblasts. Furthermore, hepatocytes isolated from c-kit mutant rats (Ws/Ws), which are defective in proliferation of oval cells, showed essentially the same phenotypic changes as those isolated from control rats. The bile duct-like differentiation of hepatocytes was associated with increased expression of Jagged1, Jagged2, Notch1, and several Notch target genes. CK19 expression and branching morphogenesis were inhibited by dexamethasone, a mitogen-activated protein kinase kinase 1 (MEK1) inhibitor (PD98059), and a phosphatidyl inositol 3-kinase inhibitor (LY294002). After being cultured for more than 3 weeks within the gels, hepatocytes transformed into ductular structures surrounded by basement membranes. Our results suggest that hepatocytes might have the potential to transdifferentiate into bile duct-like cells without acquiring a stem-like phenotype and that this is mediated through specific protein tyrosine phosphorylation pathways.

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Section: Gene Expression Of Bile Duct-specific Proteins As Well As Omentioning

confidence: 96%

Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro

Nishikawa¹,

Doi²,

Watanabe³

et al. 2005

The American Journal of Pathology

103

View full text Add to dashboard Cite

show abstract

Developmental changes in glutathione S-transferase isoforms expression and activity in intrasplenic fetal liver tissue transplants in rats

Cited by 1 publication

References 26 publications

Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro

Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro

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