Developmental Changes in Spermatogenesis, Testicular Carnitine Acetyltransferase Activity and Serum Testosterone in the Ram1

Schanbacher, B. D.; Gomes, W. R.; Vandemark, N. L.

doi:10.2527/jas1974.395889x

Cited by 18 publications

(9 citation statements)

References 10 publications

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“…All stages of spermatogenesis were evident when the level of testicular steroids reached a higher level, as indicated by the peak of seminal vesicular activity at 232 days of age. These results agree with those reported by SCHANBACHER et al (1974).…”

Section: Microscopic Examinationsupporting

confidence: 94%

Puberty in Awassi ram lambs

Ghannam

Madhat

Eltawil

1977

Zeitschrift für Tierzüchtung und Züchtungsbiologie

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Section: Microscopic Examinationsupporting

confidence: 94%

Puberty in Awassi ram lambs

Ghannam

Madhat

Eltawil

1977

Zeitschrift für Tierzüchtung und Züchtungsbiologie

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“…This may be due to the observations starting too late and not being frequent enough to reveal changes in testicular morphology and the first appearance of spermatozoa in the ejaculates (Schanbacher et al, 1974;Lee et al, 1976a).…”

Section: Discussionmentioning

confidence: 83%

Reproductive characteristics of lambs actively immunized early in life with inhibin-enriched preparations from follicular fluid of cows

Al-Obaidi

Bindon²,

Hillard³

et al. 1987

Reproduction

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Active immunization of Merino and crossbred ewe lambs early in life with a partly purified inhibin derived from bovine follicular fluid (bFF) advanced their puberty. This occurred whether the lambs were immunized from 3 weeks of age or from 9 weeks of age or just 3 times between 3 and 9 weeks. However, the effect was more obvious with multiple injections starting at 3 weeks of age. The ovulation rate of the immunized lambs was significantly higher than that of the control ewes. When lambs were subjected to multiple immunizations the increased ovulation rate persisted in Merinos for at least 1 year after immunization ceased. The lambs injected 3 times only (at 3, 6 and 9 weeks of age) did not show any increase in ovulation rate. Plasma concentrations of FSH and LH measured in blood samples taken 1 week after immunization were not significantly different between the treatment groups. Daily sperm output in the urine and testis diameter were measured in ram lambs from the same breeds and flocks as the ewe lambs. Immunization with the inhibin preparation from 3 weeks of age resulted in a significant increase in daily sperm output (4255 +/- 701 vs 2344 +/- 344 x 10(6), P less than 0.01) and testis diameter (5.21 +/- 0.3 vs 4.33 +/- 0.2 cm, P less than 0.05) in Merino rams examined in the non-breeding season when the rams were aged 23 months. Although the same trend was seen in the following year the differences were not significant. Immunization from 9 weeks of age had no effect. A similar increase in daily sperm output was seen (at 20 and 25 months of age) in crossbred rams immunized from 3 weeks of age but the difference was not significant. Plasma concentrations of FSH (but not LH) in samples from rams immunized from 3 weeks of age were significantly higher than those of control rams at 7 and 60 weeks of age in Merino rams and at 23 and 31 weeks of age in crossbred rams. Plasma FSH concentrations at 21 and 26 weeks of age in the Merino rams immunized from 3 weeks of age were higher than those of control lambs, and these increases preceded a significant (P less than 0.05) increase in testicular diameter at 30 weeks of age (4.99 +/- 0.2 vs 4.37 +/- 0.2 cm). These results suggest that active immunization early in life with an inhibin-enriched fraction from bFF advances puberty in ewe lambs.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Administration of glucocorticoid in utero influences testicular development in the sheep foetus, and the effects carry over to post-natal development. There was a reduction in sex cord diameter and volume densities that persisted long into post-natal pre-pubertal development, with a delay in opening of the sex cords as they develop into functional seminiferous tubules typically around day 90 after birth in the male sheep (Schanbacher et al 1974). These histological changes were accompanied by changes in the balance between apoptosis-inhibiting and apoptosis-inducing proteins as the testis develops, suggesting that the glucocorticoids have immediate and long-term effects on the processes that modify tissue structure during the development of spermatogenic function.…”

Section: Discussionmentioning

confidence: 99%

“…It is feasible that in utero glucocorticoid treatment affects future fertility. Overall, the histological evidence is that pre-natal glucocorticoid treatment delays pre-pubertal testicular development, the consequences of which would be postponement of the first spermatogenic wavenormally, gonocytes begin to form spermatogonia at 65 post-natal days, and primary spermatocytes are evident at 69 postnatal days (Schanbacher et al 1974). During foetal life, an increase in sex cord size is mainly a consequence of increase in sex cord length, not diameter (Hochereau-de Reviers et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Apoptosis‐Related Protein Expression During Pre‐ and Post‐Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep

Pedrana

Viotti

Souza

et al. 2013

Reprod Domestic Animals

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Pre-natal glucocorticoids are used in women at risk of preterm delivery to induce foetal lung maturation. However, glucocorticoids can produce negative outcomes for other tissues such as the reproductive system. We therefore tested the effects of pre-natal betamethasone on testicular morphology and apoptotic protein immune expression during pre- and post-natal development. Pregnant ewes (n = 42) bearing singleton male foetuses were randomly allocated to receive intramuscular injections of saline or betamethasone (0. 5 mg/kg) at 104, 111 and 118 days of gestation (DG). Testes were collected at 121 and 132 DG, and at 45 and 90 post-natal days (PD) and subjected to morphometric analysis (volume densities of sex cords and interstitial tissues; sex cord diameter). Immunohistochemistry (% stained area) was used to assess active caspase-3, Bax, Bcl-2 and cell-cycle proteins (PCNA). Compared with control values, betamethasone treatment decreased sex cord diameter at 121 DG, 45 and 90 PD, and sex cord volume at 90 PD. Active caspase-3 was decreased by betamethasone at 121 DG and 90 PD, but Bax was increased in all betamethasone groups. Bcl-2 and PCNA decreased in the betamethasone groups at 121 DG and 45 PD, but increased at 132 DG and 90 PD. We conclude that high levels of pre-natally administered glucocorticoid reduce foetal testicular development, perhaps via changes in the balance between pro- and anti-apoptotic proteins and cell-cycle proteins. These outcomes could compromise the future spermatogenic potential of male offspring.

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Developmental Changes in Spermatogenesis, Testicular Carnitine Acetyltransferase Activity and Serum Testosterone in the Ram1

Cited by 18 publications

References 10 publications

Puberty in Awassi ram lambs

Puberty in Awassi ram lambs

Reproductive characteristics of lambs actively immunized early in life with inhibin-enriched preparations from follicular fluid of cows

Apoptosis‐Related Protein Expression During Pre‐ and Post‐Natal Testicular Development After Administration of Glucocorticoid in utero in the Sheep

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