Developmental characteristics of AMPA receptors in chick lumbar motoneurons

Ni, Xianglian; Sullivan, Grace J.; Martin‐Caraballo, Miguel

doi:10.1002/dneu.20517

Cited by 11 publications

(23 citation statements)

References 55 publications

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“…(57) In the developing chick embryo, a reduction in calcium permeability over days E6 and E11 is accompanied by an increase in GluR2 expression. (58) The Drosophila ADAR is also highly developmentally regulated. (59) Thus, changes in calcium permeability resulting from editing may match changes in the functional roles of receptors.…”

Section: Glur7 Nonementioning

confidence: 99%

Edited GluR2, a gatekeeper for motor neurone survival?

et al. 2008

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Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disorder of motor neurones. Although the genetic basis of familial forms of ALS has been well explored, the molecular basis of sporadic ALS is less well understood. Recent evidence has linked sporadic ALS with the failure to edit key residues in ionotropic glutamate receptors, resulting in excessive influx of calcium ions into motor neurones which in turn triggers cell death. Here we suggest that edited AMPA glutamate (GluR2) receptor subunits serve as gatekeepers for motor neurone survival.

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Section: Glur7 Nonementioning

confidence: 99%

Edited GluR2, a gatekeeper for motor neurone survival?

et al. 2008

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“…without conversion to absolute values of intracellular free Ca 2? [15]. and Bad to generate relative expression levels.…”

Section: Mitochondrial Membrane Potential (Dw) Studiesmentioning

confidence: 99%

Tanshinone IIA activates calcium-dependent apoptosis signaling pathway in human hepatoma cells

et al. 2011

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Tanshinone IIA (Tan IIA), a natural product from herb Salvia miltiorrhiza Bunge, has potential anti-tumor activity. The aim of this study was to pinpoint the molecular mechanisms underlying Tan IIA-induced cancer cell apoptosis. Human hepatoma BEL-7402 cells treated with Tan IIA underwent assessment with MTT assay for cell viability, 10-day culture for colony formation, flow cytometry and fluorescence microscopy for apoptosis and cell cycle analysis. Changes in intracellular [Ca(2+)] and mitochondrial membrane potential (∆ψ) reflected the calcium-dependent apoptosis pathway. RT-PCR was used to detect gene expression of Bad and metallothionein 1A (MT 1A). Cytotoxicity of Tan IIA was tested in human amniotic mesenchymal stem cells (HAMCs). Tan IIA exhibited dose-dependent and time-dependent anticancer effects on BEL-7402 cells through apoptosis and G(0)/G(1) arrest. Cells treated with Tan IIA increased their intracellular calcium, decreased their mitochondrial membrane potential and induced Bad and MT 1A mRNA expression. No adverse effects of Tan IIA were found in HAMCs. In conclusion, these results indicate that Tan IIA-induced cancer cell apoptosis acts via activation of calcium-dependent apoptosis signaling pathways and upregulation of MT 1A expression.

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“…Finally, it is possible that another glutamate receptor, kainate (KA), is present in chick EW neurons. KA receptors are abundant in the avian CNS [6,19], and prior studies by others have shown that KA-evoked currents transiently mediate excitatory transmission in chick lumbar motoneurons early in development [19]. …”

mentioning

confidence: 99%

“…Composition of the ionotropic AMPA-type glutamate receptor subunit determines the functional properties of the receptor, including calcium ion permeability [12,14,19]. GluR2 restricts calcium entry through the AMPA receptor channel [12,14,19].…”

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confidence: 99%

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Immunohistochemical localization of AMPA-type glutamate receptor subunits in the nucleus of the Edinger-Westphal in embryonic chick

Toledo

Reiner

Patel

et al. 2011

Neuroscience Letters

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The Edinger-Westphal nucleus (EW) in birds is responsible for the control of pupil constriction, accommodation, and choroidal blood flow. The activation of EW neurons is mediated by the neurotransmitter glutamate, in large part through AMPA-type glutamate receptors (GluRs), whose behavior varies according to the subunit composition. We investigated the developmental expression of the GluR subunits in EW of the chick (Gallus gallus) using immunohistochemistry on tissue from embryonic days 10 through 20 (E10–E20). Of the three antibodies used, one recognized the GluR1 subunit, another the GluR4 subunit, and the third recognized a sequence common to GluR2 and GluR3 subunits. No immunolabeling of EW neurons for any GluR subunits was observed prior to E12, although immunolabeling was seen in somatic oculomotor prior to E12. At E12, immunoreactivity for each of the three antibodies was in only approximately 2% of EW neurons. By E14, the abundance of GluR1+ perikarya in EW had increased to 13%, and for GluR2/3 had increased to 48%. The perikaryal abundance of the immunoreactivity for GluR1 and GluR2/3 declined to 3% and 23%, respectively, by E16. At E14, 33% of EW neurons immunolabeled for GluR4, and their frequency increased to 43% by E16, and remained at that approximate percentage through hatching. The increased expression of GluR1 and GluR4 in EW at E14 coincides with the reported onset of the expression of the calcium-binding protein parvalbumin, and the calcium currents associated with AMPA receptors formed by these two subunits may play a role in the occurrence of parvalbumin expression.

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Developmental characteristics of AMPA receptors in chick lumbar motoneurons

Cited by 11 publications

References 55 publications

Edited GluR2, a gatekeeper for motor neurone survival?

Edited GluR2, a gatekeeper for motor neurone survival?

Tanshinone IIA activates calcium-dependent apoptosis signaling pathway in human hepatoma cells

Immunohistochemical localization of AMPA-type glutamate receptor subunits in the nucleus of the Edinger-Westphal in embryonic chick

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