Developmental clock and mechanism of de novo polarization of the mouse embryo

Zhu, Meng; Cornwall-Scoones, Jake; Wang, Peizhe; Handford, Charlotte E.; Na, Jie; Thomson, Matt; Zernicka‐Goetz, Magdalena

doi:10.1126/science.abd2703

Cited by 85 publications

(87 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although our piece particularly focuses on aspects of timing in de novo cellularization, similar questions on cell cycle autonomous timing can be proposed for other morphogenesis phenomena in early development. Indeed, a recent work has revealed the molecular basis of a long-suspected developmental clock that triggers apicobasal cell polarization independently of cell cycle progression in mice embryos ( Zhu et al, 2020 ; Figure 3B ), strongly supporting the possibility of other autonomous timing mechanisms in development and morphogenesis. Such temporal mechanisms could even contribute to differences in the developmental timing of various embryonic structures (i.e., heterochrony), contributing to much of the diversity in body shapes throughout evolution.…”

Section: The Emerging Concept Of Autonomous Clocks and Their Mechanisms At Different Scales And Complexitiesmentioning

confidence: 69%

Autonomous clocks that regulate organelle biogenesis, cytoskeletal organization, and intracellular dynamics

Mofatteh

Iturra

Alamban

et al. 2021

eLife

View full text Add to dashboard Cite

How do cells perceive time? Do cells use temporal information to regulate the production/degradation of their enzymes, membranes, and organelles? Does controlling biological time influence cytoskeletal organization and cellular architecture in ways that confer evolutionary and physiological advantages? Potential answers to these fundamental questions of cell biology have historically revolved around the discussion of ‘master’ temporal programs, such as the principal cyclin-dependent kinase/cyclin cell division oscillator and the circadian clock. In this review, we provide an overview of the recent evidence supporting an emerging concept of ‘autonomous clocks,’ which under normal conditions can be entrained by the cell cycle and/or the circadian clock to run at their pace, but can also run independently to serve their functions if/when these major temporal programs are halted/abrupted. We begin the discussion by introducing recent developments in the study of such clocks and their roles at different scales and complexities. We then use current advances to elucidate the logic and molecular architecture of temporal networks that comprise autonomous clocks, providing important clues as to how these clocks may have evolved to run independently and, sometimes at the cost of redundancy, have strongly coupled to run under the full command of the cell cycle and/or the circadian clock. Next, we review a list of important recent findings that have shed new light onto potential hallmarks of autonomous clocks, suggestive of prospective theoretical and experimental approaches to further accelerate their discovery. Finally, we discuss their roles in health and disease, as well as possible therapeutic opportunities that targeting the autonomous clocks may offer.

show abstract

Section: The Emerging Concept Of Autonomous Clocks and Their Mechanisms At Different Scales And Complexitiesmentioning

confidence: 69%

Autonomous clocks that regulate organelle biogenesis, cytoskeletal organization, and intracellular dynamics

Mofatteh

Iturra

Alamban

et al. 2021

eLife

View full text Add to dashboard Cite

show abstract

“…In vivo, ezrin is found as oligomers, and more generally in clusters, in addition to monomeric forms (39)(40)(41). Thus, we next tested the binding of multiple ezrin-T567D molecules to F-actin.…”

Section: Resultsmentioning

confidence: 99%

The membrane-actin linker ezrin acts as a sliding anchor

Korkmazhan

Dunn

2021

Preprint

View full text Add to dashboard Cite

Protein linkages to filamentous (F)-actin provide the cell membrane with mechanical resiliency and give rise to intricate membrane architectures. However, the actin cytoskeleton is highly dynamic, and undergoes rapid changes in shape during cell motility and other processes. The molecular mechanisms that underlie the mechanically robust yet fluid connection between the membrane and actin cytoskeleton remain poorly understood. Here, we used a single-molecule optical trap assay to examine how the prototypical membrane-actin linker ezrin acts to anchor F-actin to the cell membrane. Remarkably, we find that ezrin forms a complex that slides along F-actin over micron distances while resisting mechanical detachment. The ubiquity of ezrin and analogous proteins suggests that sliding anchors such as ezrin may constitute an important but overlooked element in the construction of the actin cytoskeleton.

show abstract

“…Apical domain formation is an essential process for embryo polarization to induce the segregation of the ICM and TE lineages (Korotkevich et al, 2017). In this process, the ERM proteins (Ezr, Radixin, and Moesin) and Par protein complex surrounded by an actomyosin ring migrate to the apical region of polar cells in mice (Plusa et al, 2005; Zhu et al, 2020). Hippo pathway is involved in the regulation of the expression of important polar proteins (Rossant, 2018).…”

Section: Resultsmentioning

confidence: 99%

Linker histone H1FOO is required for bovine preimplantation development by regulating lineage specification and nucleosome assembly

Shi

Dang

et al. 2021

Preprint

View full text Add to dashboard Cite

Linker histone H1 binds to the nucleosome and is implicated in the regulation of the chromatin structure and function. The H1 variant H1FOO is heavily expressed in oocytes and early embryos. However, given the poor homology of H1FOO among mammals, the functional role of H1FOO during early embryonic development remains largely unknown, especially in domestic animals. Here, we find that H1FOO is not only expressed in oocytes and early embryos but granulosa cells and spermatids in cattle. We then demonstrate that the interference of H1FOO results in early embryonic developmental arrest in cattle using either RNA editing or Trim-Away approach. H1FOO depletion leads to compromised expression of critical lineage-specific genes at the morula stage and affects the establishment of cell polarity. Interestingly, H1FOO depletion causes a significant increase in expression genes encoding other linker H1 and core histones. Concurrently, there is an increase of H3K9me3 and H3K27me3, two markers of repressive chromatin and a decrease of H4K16ac, a marker of open chromatin. Importantly, overexpression of bovine H1FOO results in severe embryonic developmental defects. In sum, we propose that H1FOO controls the proper chromatin structure that is crucial for the fidelity of cell polarization and lineage specification during bovine early development.

show abstract

Developmental clock and mechanism of de novo polarization of the mouse embryo

Cited by 85 publications

References 69 publications

Autonomous clocks that regulate organelle biogenesis, cytoskeletal organization, and intracellular dynamics

Autonomous clocks that regulate organelle biogenesis, cytoskeletal organization, and intracellular dynamics

The membrane-actin linker ezrin acts as a sliding anchor

Linker histone H1FOO is required for bovine preimplantation development by regulating lineage specification and nucleosome assembly

Contact Info

Product

Resources

About