Developmental competence and gene expression of immature oocytes following liquid helium vitrification in bovine

Chen, Junyi; Li, Xiaoxia; Xu, Yifan; Wu, Hua; Zheng, Jiong; Xiang, Yu

doi:10.1016/j.cryobiol.2014.09.380

Cited by 23 publications

(10 citation statements)

References 36 publications

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“…In a previous study [16] rates were recorded in our study [16]. Our study is also concluded The levels of HSP90 mRNA and proteins were significantly lower pro-apoptotic gene, stress gene and so on [3]. HSP90 is also as a 440 stress gene that helps cells to adapt to environment changes.…”

supporting

confidence: 52%

“…68 Since the 1980s, oocyte cryopreservation studies have increased 69 gradually, and most of those studies have focused on metaphase II 70 (MII). However, studies of the cryopreservation of immature 71 oocytes are limited [3,25] [4,28,29]. SSV is an inexpensive method 75 that reaches high cooling rates using a combination of microdrops 76 and improved heat exchange through direct contact with a dry 77 metal surface that is cooled by liquid nitrogen (LN) [6].…”

mentioning

confidence: 99%

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Association of heat shock protein 90 with the developmental competence of immature oocytes following Cryotop and solid surface vitrification in yaks (Bos grunniens)

et al. 2015

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supporting

confidence: 52%

mentioning

confidence: 99%

Association of heat shock protein 90 with the developmental competence of immature oocytes following Cryotop and solid surface vitrification in yaks (Bos grunniens)

et al. 2015

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“…Pro‐apoptotic gene such as Bax can promote the release of cytochrome c from mitochondria into the cytoplasm, and anti‐apoptotic gene such as Bcl2 l1 inhibits the release of cytochrome c from mitochondria, consequently preventing apoptosis. Vitrification has been demonstrated to promote the mRNA expression levels of Bax and caspase‐3 in mouse oocytes and Bax in bovine oocytes . As shown in Fig.…”

Section: Discussionmentioning

confidence: 96%

Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes

Zhao

Huang

et al. 2015

Journal of Pineal Research

139

104

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Vitrification of oocytes has been shown to be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. However, little information is available the effect of melatonin on the ROS levels and apoptotic events in vitrified oocytes. Therefore, we studied the effect of melatonin on ROS and apoptotic events in vitrified bovine oocytes by supplementing vitrification solution or in vitro maturation (IVM) and vitrification solution with 10(-9) m melatonin. We analyzed the ROS, mitochondrial Ca(2+) (mCa(2+) ) and membrane potential (ΔΨm), externalization of phosphatidylserine (PS), caspase-3 activation, DNA fragmentation, mRNA expression levels of Bax and Bcl2 l1, and developmental potential of vitrified bovine oocytes. Vitrified bovine oocytes exhibited increased levels of ROS, mCa(2+) , Bax mRNA, and caspase-3 protein and higher rates of PS externalization and DNA fragmentation, and decreased ΔΨm and Bcl2 l1 mRNA expression level. However, melatonin supplementation in vitrification solution or IVM and vitrification solution significantly decreased the levels of ROS, mCa(2+) , Bax mRNA expression, and caspase-3 protein, and PS externalization and DNA fragmentation rates, and increased the ΔΨm and Bcl2 l1 mRNA expression level in vitrified oocytes, resulting in an increased developmental ability of vitrified bovine oocytes after parthenogenetic activation. The developmental ability of vitrified oocytes with melatonin supplementation in IVM and vitrification solution was similar to that of fresh ones. This study showed that supplementing the IVM and vitrification medium or vitrification medium with 10(-9) m melatonin significantly decreased the ROS level and inhibited apoptotic events of vitrified bovine oocytes, consequently increasing their developmental potential.

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“…Rao et al (2012) juga mendapatkan hasil yang sama, bahwasanya vitrifikasi oosit immatur dengan menggunakan hemistraw tidak berpengaruh pada ekspresi GDF9, akan tetapi ekspresi GDF9 yang tinggi justru menurunkan angka maturasi oosit. Chen et al (2014) juga mendapatkan hasil yang sama, bahwa vitrifikasi pada oosit immatur menunjukkan peningkatan ekspresi GDF9, tapi didapatkan penurunan morfologi oosit, maturasi, dan kemampuan perkembangan oosit pada tahap selanjutnya. Hal ini menunjukkan bahwa peningkatan ekspresi GDF9 tidak mampu menjaga viabilitas dan perkembangan oosit setelah proses vitrifikasi, akan tetapi pada penelitian ini tidak dilakukan penilaian terhadap maturasi dan morfologi oosit.…”

Section: Penelitianunclassified

EXPRESSION OF TRANSFORMING GROWTH FACTOR-beta AND GROWTH DIFFERENTIATION FACTOR-9 ON SHEEP OOCYTES VITRIFIED AFTER AND BEFORE IN VITRO MATURATION

Faizah

Aswin

2021

jveteriner

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Oocyte vitrification today became a hope to preserve fertility. Its was a major challenge because of oocyte characteristic in every phase. Immature oocytes were more sensitive to osmotic stress and the membrane wes less stable while mature oocyte have spindles that were very susceptible to temperature decrease. The study aim to compare the effect of vitrification before and after in vitro maturation to the expression TGF beta and GDF9. Oocyte of ewes divided into control groups (K0), K1 maturation prior vitrification, K2 vitrification prior maturation. Vitrification begins with washing oocytes in PBS supplemented of 20%serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2).PBS + 20% serum + 0.25 M sucrose, and 3).PBS + 20% serum + 0.1 M sucrose. Immunocytochemical stain was performed to evaluate TGF ? and GDF9 expression. Remmele scale index (IRS) was used to read the result. TGF beta expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. GDF9 expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. We concluded that immature oocyte give better expression of TGF â and GDF9 than mature oocyte.

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Developmental competence and gene expression of immature oocytes following liquid helium vitrification in bovine

Cited by 23 publications

References 36 publications

Association of heat shock protein 90 with the developmental competence of immature oocytes following Cryotop and solid surface vitrification in yaks (Bos grunniens)

Association of heat shock protein 90 with the developmental competence of immature oocytes following Cryotop and solid surface vitrification in yaks (Bos grunniens)

Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes

EXPRESSION OF TRANSFORMING GROWTH FACTOR-beta AND GROWTH DIFFERENTIATION FACTOR-9 ON SHEEP OOCYTES VITRIFIED AFTER AND BEFORE IN VITRO MATURATION

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