Developmental competence of oocytes after ICSI in the rhesus monkey

Nusser, Kevin D.; Mitalipov, Shoukhrat; Widmann, Andrea A.; Gerami‐Naini, Behzad; Yeoman, Richard R.; Wolf, Don P.

doi:10.1093/humrep/16.1.130

Cited by 51 publications

(35 citation statements)

References 30 publications

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“…A significant limitation on the feasibility of this approach has been the difficulties with the transfer of blastocyst-stage embryos in rhesus monkeys, the most widely used nonhuman primate model. Only recently have parameters of successful implantation (e.g., timing of embryo transfer) been examined (20,29) and the birth of rhesus monkeys after transcervical transfer of blastocyst-stage embryos been achieved. We have shown in the current studies that transcervical blastocyst transfer has proven to be a reliable means of obtaining live rhesus infants even after extensive manipulation in vitro such as micromanipulation, viral genetic modification, and fluorescent imaging.…”

Section: Discussionmentioning

confidence: 99%

Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos

Wolfgang

Eisele

Browne

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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Transgenic mice have provided invaluable information about gene function and regulation. However, because of marked differences between rodents and primates, some areas of human biology such as early embryonic development, aging, and maternal-fetal interactions would be best studied in a nonhuman primate model. Here, we report that gene transfer into rhesus monkey (Macaca mulatta) preimplantation embryos gives rise to transgenic placentas that express a reporter transgene (eGFP). Blastocysts resulting from culture of in vitro fertilized ova were transduced with a selfinactivating lentiviral vector and transferred into recipient females. One twin and one singleton pregnancy were produced from a single stimulation cycle, and one live rhesus monkey was born from each pregnancy. Placentas from all conceptuses showed expression of the transgene as detected by reverse transcription-PCR, ribonuclease protection assay, direct epifluorescence, immunohistochemistry, and Western blot analysis. Integration in somatic tissues of the offspring was not detected. A maternal immune response to the xenogeneic placental antigen was shown by the presence of anti-GFP antibodies in peripheral blood of the recipient females by day 99 of gestation (term ‫؍‬ 165 days). These results demonstrate that transgene expression during gestation is compatible with successful pregnancy in nonhuman primates and provides an approach that could be broadly applicable to the development of novel models for primate biomedical research.

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Section: Discussionmentioning

confidence: 99%

Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos

Wolfgang

Eisele

Browne

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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“…In vitro culturing with and without a MEF cell monolayer supported development to the blastocyst stage, while culturing with a BRL cell monolayer did not. Although a BRL cell monolayer supported development to the blastocyst stage of rhesus monkey embryos (Zhang et al 1994, Nusser et al 2001, the reason for this negative effect by BRL cells in African green monkey embryos in the present study is uncertain. On the other hand, the ES cell line was established from the blastocysts that originated from the culture system with the MEF cell monolayer.…”

Section: Discussionmentioning

confidence: 53%

“…However, the in vitro culturing for the African green monkey (Cercopithecus aethiops) embryos has not been examined. In rhesus monkey embryos, co-culture with a buffalo rat liver (BRL) cell monolayer in CMRL-1066 has been utilized (Zhang et al 1994, Nusser et al 2001. On the other hand, mouse embryonic fibroblast (MEF) cells have been widely utilized for the establishment of embryonic stem (ES) cells, showing that MEF cells affect establishment of the ES cells from the inner cell mass (ICM), where develop to an individual in the future, of blastocyst.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey

Shimozawa¹,

Nakamura²,

Takahashi³

et al. 2010

Reproduction

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Several cell types from the African green monkey (Cercopithecus aethiops), such as red blood cells, primary culture cells from kidney, and the Vero cell line, are valuable sources for biomedical research and testing. Embryonic stem (ES) cells that are established from blastocysts have pluripotency to differentiate into these and other types of cells. We examined an in vitro culture system of zygotes produced by ICSI in African green monkeys and attempted to establish ES cells. Culturing with and without a mouse embryonic fibroblast (MEF) cell monolayer resulted in the development of ICSI-derived zygotes to the blastocyst stage, while culturing with a buffalo rat liver cell monolayer yielded no development (3/14, 21.4% and 6/31, 19.4% vs 0/23, 0% respectively; P!0.05). One of the nine blastocysts, which had been one of the zygotes co-cultured with MEF cells, formed flat colonies consisting of cells with large nuclei, similar to other primate ES cell lines. The African green monkey ES (AgMES) cells expressed pluripotency markers, formed teratomas consisting of three embryonic germ layer tissues, and had a normal chromosome number. Furthermore, expression of the germ cell markers CD9 and DPPA3 (STELLA) was detected in the embryoid bodies, suggesting that AgMES cells might have the potential ability to differentiate into germ cells. The results suggested that MEF cells greatly affected the quality of the inner cell mass of the blastocysts. In addition, AgMES cells would be a precious resource for biomedical research such as other primate ES cell lines.

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“…Since the first birth of a rhesus macaque after IVF in the mid-1980s (1), several laboratories have reported successfully obtaining infants of rhesus, cynomolgus monkey, and pigtailed macaque by IVF or ICSI (8)(9)(10)(11)(12)(13)(14). However, the success rate has been low in comparison with that for rodents and humans.…”

Section: Discussionmentioning

confidence: 99%

Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique

Sun

Dong

Yang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo transfer can be used as an effective means for improving pregnancy and live birth rates of nonhuman primates. We collected 174 metaphase II oocytes by laparoscopy from 22 superovulated mature females and then fertilized these eggs using either in vitro fertilization or intracytoplasmic sperm injection, resulting in a 33.3% and a 50% fertilization rate, respectively. These 66 fertilized pronuclear-stage embryos were then tubally transferred to 30 recipients and led to 7 births and 1 abortion. Importantly, we observed that the highest live birth rate of Ϸ64% was obtained when the transfer of pronuclear embryos was performed in the presence of new corpus luteum in the ovary of recipients between 24 h and 36 h after estradiol peak. Therefore, our experiments demonstrate that by matching the critical time window in the recipient's reproductive cycle for achieving optimal embryo-uterine synchrony, pronuclear embryo transfer technology can significantly improve the pregnancy rate and live birth of healthy baby monkeys. This efficient method should be valuable to the systematic efforts in construction of various transgenic primate disease models.in vitro fertilization ͉ intracytoplasmic sperm injection ͉ test-tube monkey ͉ transgenic monkey ͉ embryo-uterine synchrony C onsidering the great genetic and physiologic similarities with humans, nonhuman primates such as monkeys represent the most ideal experimental models for detailed analysis of biologic processes under physiologic or pathologic conditions. As a result, new drug candidates are typically required to go through systematic assessment of drug efficacy, pharmacokinetics, and toxicity in monkeys before their evaluation in human clinical trials. Furthermore, monkeys are the most favored organisms for in-depth analysis of neural mechanisms underlying high cognition and complex behavior. It is conceivable that the potential of the nonhuman primate model systems can be further expanded when researchers introduce or remove genes of interest by transgenic methods that have been proven so powerful in lower organisms such as mice, zebra fish, and fruit flies.One of the major bottlenecks in producing transgenic monkeys is the low birth rate of ''test-tube'' baby monkeys with assisted reproductive technologies. Although the first birth of a monkey (a rhesus macaque) after in vitro fertilization (IVF) was reported in 1984 (1), constraints on materials and resources for systematic experimentation with various in vitro culture conditions and procedures have contributed to the slow progress of the field. There are also many factors that can greatly affect the quality of embryo development and the rates of pregnancy and live birth. For example, the success of IVF meth...

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Developmental competence of oocytes after ICSI in the rhesus monkey

Cited by 51 publications

References 30 publications

Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos

Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos

Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey

Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique

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