Developmental Control of Stress Stimulons in Streptomyces coelicolor Revealed by Statistical Analyses of Global Gene Expression Patterns

Abstract

Stress-induced regulatory networks coordinated with a procaryotic developmental program were revealed by two-dimensional gel analyses of global gene expression. Four developmental stages were identified by their distinctive protein synthesis patterns using principal component analysis. Statistical analyses focused on five stress stimulons (induced by heat, cold, salt, ethanol, or antibiotic shock) and their synthesis during development. Unlike other bacteria, for which various stresses induce expression of similar sets of protein spots, in Streptomyces coelicolor heat, salt, and ethanol stimulons were composed of independent sets of proteins. This suggested independent control by different physiological stress signals and their corresponding regulatory systems. These stress proteins were also under developmental control. Cluster analysis of stress protein synthesis profiles identified 10 different developmental patterns or "synexpression groups." Proteins induced by cold, heat, or salt shock were enriched in three developmental synexpression groups. In addition, certain proteins belonging to the heat and salt shock stimulons were coregulated during development. Thus, stress regulatory systems controlling these stimulons were implicated as integral parts of the developmental program. This correlation suggested that thermal shock and salt shock stress response regulatory systems either allow the cell to adapt to stresses associated with development or directly control the developmental program.

Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways inStreptomyces coelicolorusing DNA microarrays

“…1), as reported previously (Leskiw et al 1993;Puglia et al 1995;Vohradsky et al 2000). The antibiotics CDA and Red were first detected during T phase, whereas Act production was not detectable until S phase (Fig.…”

An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2)

“…S. coelicolor wild-type strain 1147 grew rapidly in YEME medium with a temporary (2 h) cessation of growth at the mid-exponential growth phase (Fig. 1a), possibly due to depletion of the nitrogen source, as previously pointed out by Vohradsky et al (2000). Later (at 42 h), cells entered into the stationary phase.…”

The phosphotransferase system of Streptomyces coelicolor

“…They suggested that this could be a linkage between carbon metabolism and stress response upon nutrient starvation. Thus, IIA Crr could be involved in controlling some of the many sigma factors that S. coelicolor possesses [4,41]. Further analyses are required to address the ideas mentioned above.…”

“…A specific interaction of IIA Crr with the maltose permease subunit MalK from Salmonella typhimurium was uncovered by surface plasmon resonance. These data suggest that this IIA Glucose -like protein may be involved in carbon metabolism in S. coelicolor.Keywords: inducer exclusion; protein phosphorylation; protein-protein interaction; Streptomyces; surface plasmon resonance.Streptomycetes undergo global changes in gene expression and enzyme activities in response to developmental stages, secondary metabolite production (antibiotics), carbon utilization, and stress conditions [1][2][3][4][5]. The focus of our research is the regulation of carbon source utilization (C-regulation) and how this influences the other abovementioned processes.…”

“…Streptomycetes undergo global changes in gene expression and enzyme activities in response to developmental stages, secondary metabolite production (antibiotics), carbon utilization, and stress conditions [1][2][3][4][5]. The focus of our research is the regulation of carbon source utilization (C-regulation) and how this influences the other abovementioned processes.…”