Developmental Decisions in Aspergillus nidulans

Park, Hee-Soo; Lee, Mi-Kyung; Han, Kap-Hoon; Kim, Min-Ju; Yu, Jae‐Hyuk

doi:10.1007/978-3-030-05448-9_4

Cited by 16 publications

(19 citation statements)

References 155 publications

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“…The processes of sexual and asexual structure formation are complicated and are tightly regulated by a variety of regulators [4,11]. These studies have primarily focused on the model fungus Aspergillus nidulans [12,13].…”

Section: Introductionmentioning

confidence: 99%

The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

Kim

Lee

Pham

et al. 2020

Genes

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The velvet regulator VosA plays a pivotal role in asexual sporulation in the model filamentous fungus Aspergillus nidulans. In the present study, we characterize the roles of VosA in sexual spores (ascospores) in A. nidulans. During ascospore maturation, the deletion of vosA causes a rapid decrease in spore viability. The absence of vosA also results in a lack of trehalose biogenesis and decreased tolerance of ascospores to thermal and oxidative stresses. RNA-seq-based genome-wide expression analysis demonstrated that the loss of vosA leads to elevated expression of sterigmatocystin (ST) biosynthetic genes and a slight increase in ST production in ascospores. Moreover, the deletion of vosA causes upregulation of additional gene clusters associated with the biosynthesis of other secondary metabolites, including asperthecin, microperfuranone, and monodictyphenone. On the other hand, the lack of vosA results in the downregulation of various genes involved in primary metabolism. In addition, vosA deletion alters mRNA levels of genes associated with the cell wall integrity and trehalose biosynthesis. Overall, these results demonstrate that the velvet regulator VosA plays a key role in the maturation and the cellular and metabolic integrity of sexual spores in A. nidulans.

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Section: Introductionmentioning

confidence: 99%

The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

Kim

Lee

Pham

et al. 2020

Genes

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“…After the germ tube is formed from spores, it grows into hyphae, forming the mycelium, a web-like mass of fungal hyphae [16,17]. After acquiring developmental competence, fungi can produce asexual or sexual developmental structures depending on environmental conditions [18,19]. During the formation of conidiophores (asexual structures) or sexual fruiting bodies, a variety of genes and proteins participate in these processes [20,21].…”

Section: Introductionmentioning

confidence: 99%

Characterizing the role of Zn cluster family transcription factor ZcfA in governing development in two Aspergillus species

et al. 2020

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Filamentous fungi reproduce asexually or sexually, and the processes of asexual and sexual development are tightly regulated by a variety of transcription factors. In this study, we characterized a Zn 2 Cys 6 transcription factor in two Aspergillus species, A. nidulans (AN5859) and A. flavus (AFLA_046870). AN5859 encodes a Zn 2 Cys 6 transcription factor, called ZcfA. In A. nidulans, ΔzcfA mutants exhibit decreased fungal growth, a reduction in cleistothecia production, and increased asexual reproduction. Overexpression of zcfA results in increased conidial production, suggesting that ZcfA is required for proper asexual and sexual development in A. nidulans. In conidia, deletion of zcfA causes decreased trehalose levels and decreased spore viability but increased thermal sensitivity. In A. flavus, the deletion of the zcfA homolog AFLA_046870 causes increased conidial production but decreased sclerotia production; these effects are similar to those of zcfA deletion in A. nidulans development. Overall, these results demonstrate that ZcfA is essential for maintaining a balance between asexual and sexual development and that some roles of ZcfA are conserved in Aspergillus spp.

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“…The production of conidiophores is tightly controlled by a variety of positive and negative regulators [16]. Both asexual and sexual development is regulated by several signaling pathways in Aspergillus species [17,18].…”

Section: Introductionmentioning

confidence: 99%

Function of crzA in Fungal Development and Aflatoxin Production in Aspergillus flavus

Lim

Son

Lee

et al. 2019

Toxins

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The calcineurin pathway is an important signaling cascade for growth, sexual development, stress response, and pathogenicity in fungi. In this study, we investigated the function of CrzA, a key transcription factor of the calcineurin pathway, in an aflatoxin-producing fungus Aspergillus flavus (A. flavus). To examine the role of the crzA gene, crzA deletion mutant strains in A. flavus were constructed and their phenotypes, including fungal growth, spore formation, and sclerotial formation, were examined. Absence of crzA results in decreased colony growth, the number of conidia, and sclerocia production. The crzA-deficient mutant strains were more susceptible to osmotic pressure and cell wall stress than control or complemented strains. Moreover, deletion of crzA results in a reduction in aflatoxin production. Taken together, these results demonstrate that CrzA is important for differentiation and mycotoxin production in A. flavus.

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Developmental Decisions in Aspergillus nidulans

Cited by 16 publications

References 155 publications

The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

The velvet Regulator VosA Governs Survival and Secondary Metabolism of Sexual Spores in Aspergillus nidulans

Characterizing the role of Zn cluster family transcription factor ZcfA in governing development in two Aspergillus species

Function of crzA in Fungal Development and Aflatoxin Production in Aspergillus flavus

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