Developmental distribution of CaM kinase II in the antennal lobe of the sphinx moth Manduca sexta

Lohr, Christian; Bergstein, Sandra; Hirnet, Daniela

doi:10.1007/s00441-006-0249-6

Cited by 13 publications

(14 citation statements)

References 40 publications

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“…Our results confirm that calmodulin regulates olfactory attraction during nymph development, consistent with previous reports that calmodulin mediates olfactory sensation for odorants (Mons et al ., ; Heisenberg, ). In the sphinx moth, Manduca sexta , the reorganization of antennal lobes during metamorphosis is accompanied by changing patterns of calcium signalling in glial cells (Lohr et al ., ). Calmodulin participates in processing information in the olfactory epithelium during postnatal development (Bastianelli et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Calmodulin participates in processing information in the olfactory epithelium during postnatal development (Bastianelli et al ., ). In addition, previous reports have confirmed that Ca 2+ /calmodulin‐dependent protein kinase II regulates olfactory reaction and sensitivity in insects (Lohr et al ., ). The calmodulin signalling pathway mediates the sensitivity of chemosensory signalling in olfactory bulbs (Weiss, ).…”

Section: Discussionmentioning

confidence: 97%

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Calmodulin as a downstream gene of octopamine‐OAR α1 signalling mediates olfactory attraction in gregarious locusts

Yang

et al. 2016

Insect Molecular Biology

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The migratory locust (Locusta migratoria) shows aggregative traits in nymph marching bands and swarm formations through mutual olfactory attraction of conspecifics. However, olfactory preference in different nymph stages in gregarious locusts is not sufficiently explored. In this study, we found that the nymph olfactory preference for gregarious volatiles exhibited obvious variations at different developmental stages. The gregarious locusts show attractive response to conspecific volatiles from the third stadium. Transcriptome comparison between third- and fourth-stadium nymphs showed that the G protein-coupled receptor (GPCR) pathways are significantly enriched. Amongst the genes present in GPCR pathways, the expression level of calmodulin in locust brains significantly increased from the third- to the fourth-stadium nymphs. Amongst the four octopamine receptors (OARs) belonging to the GPCR family, only OAR α1 showed similar expression patterns to those of calmodulin, and knockdown of OAR α1 reduced the expression level of calmodulin. RNA interference of calmodulin decreased locomotion and induced the loss of olfactory attraction in gregarious locusts. Moreover, the activation of OAR α1 in calmodulin-knockdown locusts did not induce olfactory attraction of the nymphs to gregarious volatiles. Thus, calmodulin as a downstream gene of octopamine-OAR α1 (OA-OAR α1) signalling mediates olfactory attraction in gregarious locusts. Overall, this study provides novel insights into the mechanism of OA-OAR α1 signalling involved in olfactory attraction of gregarious locusts.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Calmodulin as a downstream gene of octopamine‐OAR α1 signalling mediates olfactory attraction in gregarious locusts

Yang

et al. 2016

Insect Molecular Biology

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“…Glial cell migration to surround developing glomeruli is most prominent during stages 6 and 7, the stages when the mean amplitude of the Ca 2+ transients was greatest. Glial cell migration in the antennal lobe depends on voltage‐gated Ca 2+ influx into the glial cells and CaM kinase activity (Lohr et al ., 2005; Lohr et al ., 2007) as well as cholinergic signaling (this study). The possibility that local Ca 2+ signaling provides a guidance cue that indicates the direction of the migration is an appealing hypothesis, but one that needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…The slices were incubated in RNAse (0.2 mg ⁄ mL) for 1 h and stained with 20 mg ⁄ mL propidium iodide for 1 h. Slices were then embedded in glycerol and immediately scanned with a confocal microscope (LSM 510, Zeiss, Oberkochen, Germany). To demonstrate the location of the cell bodies of antennal lobe neurons and the fine structured neuropil in the glomeruli, two structures that contain high levels of CaM kinase II, we labeled these structures with an anti-CaM kinase II antibody (for details see Lohr et al, 2007).…”

Section: Injection Of D-tubocurarine Into Pupae and Propidium Iodide mentioning

confidence: 99%

Acetylcholine‐mediated axon‐glia signaling in the developing insect olfactory system

Heil

Oland

Lohr

2007

Eur J of Neuroscience

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In the olfactory system of the sphinx moth Manduca sexta, migration of neuropil glial cells is triggered by olfactory receptor axons and depends on intraglial Ca(2+) signaling. It is not known, however, how receptor axons and glial cells communicate and whether Ca(2+) signaling is a consequence of this communication. We studied Ca(2+) increases in glial cells in vivo and in situ, evoked by electrical stimulation of olfactory receptor axons in pupae and by odor stimulation of receptor neurons in adult moths. Axonal activity leads to Ca(2+) increases in neuropil glial cells that are blocked by nicotinic acetylcholine receptor antagonists and can be mimicked by acetylcholine and carbachol application. In addition, Ca(2+) transients were abolished by removal of external Ca(2+) and blockage of voltage-gated Ca(2+) channels. During development, acetylcholine-mediated Ca(2+) signaling could first be elicited at stage 6, the time when neuropil glial cells start to migrate. Glial migration was reduced after injection of nicotinic antagonists into pupae. The results show that Ca(2+) signaling can be induced by acetylcholine release from olfactory receptor axons, which activates nicotinic acetylcholine receptors and leads to voltage-gated Ca(2+) influx. The results further suggest that cholinergic signaling in the olfactory system is required for glial cell migration in Manduca.

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“…Increases in intracellular calcium concentration ([Ca 2+ ] i ) are detected by multiple calcium-sensing proteins such as calcium-calmodulin-dependent protein kinase II (CaMKII). CaMKII is found in a variety of organisms and tissue types, including insect neurons (Kamikouchi et al 2000;Takamatsu et al 2003;Lohr et al 2007;Taillebois et al 2013). In the cockroach efferent dorsal unpaired median (DUM) neurons, it was proposed that CaMKII modulated nAChRs function (Thany et al 2007).…”

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confidence: 99%

Inhibition of PaCaMKII‐E isoform in the dorsal unpaired median neurosecretory cells of cockroach reduces nicotine‐ and clothianidin‐induced currents

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Taillebois

et al. 2014

Journal of Neurochemistry

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Cellular responses to Ca2+ require intermediary proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII), which transduces the signal into downstream effects. We recently demonstrated that the cockroach genome encodes five different CaMKII isoforms, and only PaCaMKII-E isoform is specifically expressed in the dorsal unpaired median neurosecretory cells. In the present study, using antisense oligonucleotides, we demonstrated that PaCaMKII-E isoform inhibition reduced nicotine-induced currents through a-bungarotoxin-sensitive and -insensitive nicotinic acetylcholine receptor subtypes. Specifically, PaCaMKII-E isoform is sufficient to repress nicotinic current amplitudes as a result of its depression by antisense oligonucleotides. Similar results were found using the neonicotinoid insecticide clothianidin, which acted as a full agonist of dorsal unpaired median neuron nicotinic acetylcholine receptors. Clothianidin current amplitudes are strongly reduced under bath application of PaCaMKII-E antisense oligonucleotides but no significant results are found with a-bungarotoxin co-applied, demonstrating that CaMKII-E isoform affects nicotine currents through a-bungarotoxin-sensitive and -insensitive receptor subtypes whereas clothianidin currents are reduced via a-bungarotoxin-insensitive receptors.In addition, we found that intracellular calcium increase induced by nicotine and clothianidin were reduced by PaCaMKII-E antisense oligonucleotides, demonstrating that intracellular calcium increase induced by nicotine and clothianidin are affected by PaCaMKII-E inhibition.

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Developmental distribution of CaM kinase II in the antennal lobe of the sphinx moth Manduca sexta

Cited by 13 publications

References 40 publications

Calmodulin as a downstream gene of octopamine‐OAR α1 signalling mediates olfactory attraction in gregarious locusts

Calmodulin as a downstream gene of octopamine‐OAR α1 signalling mediates olfactory attraction in gregarious locusts

Acetylcholine‐mediated axon‐glia signaling in the developing insect olfactory system

Inhibition of PaCaMKII‐E isoform in the dorsal unpaired median neurosecretory cells of cockroach reduces nicotine‐ and clothianidin‐induced currents

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